Question 1

You are choosing between yeast and bacterial cells as hosts for recombinant DNA.Which statement is most true?

Accepted Answer

A)  Possessing plasmids is a feature that would favor bacteria as hosts. 
B)  Possessing plasmids is a feature that would disfavor bacteria as hosts. 
C)  Differences between prokaryotes and eukaryotes often tilt the balance toward bacteria as host cells for expressing eukaryotic genes. 
D)  Bacteria are often favored as hosts because little is known about yeast genetics. 
E)  Yeasts are often favored as hosts because little is known about bacterial genetics. 
A)  Possessing plasmids is a feature that would favor bacteria as hosts. 
B)  Possessing plasmids is a feature that would disfavor bacteria as hosts. 
C)  Differences between prokaryotes and eukaryotes often tilt the balance toward bacteria as host cells for expressing eukaryotic genes. 
D)  Bacteria are often favored as hosts because little is known about yeast genetics. 
E)  Yeasts are often favored as hosts because little is known about bacterial genetics.

Question 2

Suppose a plasmid has genes that confer resistance to both ampicillin and tetracycline.Foreign DNA is inserted at a restriction enzyme recognition site that lies within the ampicillin gene.A cell transformed with the resulting recombinant plasmid should be _______ to ampicillin and _______ to tetracycline.

Accepted Answer

A)  resistant; resistant 
B)  resistant; sensitive 
C)  resistant; complementary 
D)  sensitive; resistant 
E)  sensitive; complementary 
A)  resistant; resistant 
B)  resistant; sensitive 
C)  resistant; complementary 
D)  sensitive; resistant 
E)  sensitive; complementary

Question 3

A biologist used microarrays to examine how gene expression in a fish changes with temperature.Refer to the table showing the expression levels of four (of several thousand) genes at varying temperatures.Values for expression have been normalized such that the expression at 15°C for any particular gene is always 100.   What best describes the pattern shown by gene 1053?

Accepted Answer

A)  Its expression steadily increases with temperature. 
B)  Its expression steadily decreases with temperature. 
C)  Its expression first increases and then decreases as temperature increases. 
D)  Its expression first decreases and then increases as temperature increases. 
E)  Temperature has little or no effect on its expression. 
A)  Its expression steadily increases with temperature. 
B)  Its expression steadily decreases with temperature. 
C)  Its expression first increases and then decreases as temperature increases. 
D)  Its expression first decreases and then increases as temperature increases. 
E)  Temperature has little or no effect on its expression.

Question 4

The function of DNA ligase in the generation of recombinant DNA is to _______ DNA.

Accepted Answer

A)  cut 
B)  replicate 
C)  unwind 
D)  join 
E)  copy 
A)  cut 
B)  replicate 
C)  unwind 
D)  join 
E)  copy

Question 5

A lab working with recombinant DNA includes a gene for penicillin resistance as part of the recombinant DNA.In this case, the penicillin resistance gene is acting as a(n) _______ marker.

Accepted Answer

The answer of A lab working with recombinant DNA includes...

Question 6

In the construction of a transgenic cell, a gene that enables growth on arabinose is added to the vector.This gene is being used as a(n)

Accepted Answer

A)  transformation agent. 
B)  selectable marker. 
C)  replicon. 
D)  cloning vector. 
E)  expression vector. 
A)  transformation agent. 
B)  selectable marker. 
C)  replicon. 
D)  cloning vector. 
E)  expression vector.

Question 7

Which statement concerning a genomic library is true?

Accepted Answer

A)  It comprises the genomic DNA from an organism. 
B)  It can be made only for eukaryotic organisms. 
C)  Every colony in the library has only one gene. 
D)  It can be made only from cDNA. 
E)  Reverse transcriptase is essential to construct the library. 
A)  It comprises the genomic DNA from an organism. 
B)  It can be made only for eukaryotic organisms. 
C)  Every colony in the library has only one gene. 
D)  It can be made only from cDNA. 
E)  Reverse transcriptase is essential to construct the library.

Question 8

Which assay would you most likely use to determine whether the liver, the heart, or the brain of mice is expressing more genes at a given time?

Accepted Answer

A)  CRISPR 
B)  Expression vector 
C)  Genomic library 
D)  cDNA library 
E)  RNAi 
A)  CRISPR 
B)  Expression vector 
C)  Genomic library 
D)  cDNA library 
E)  RNAi

Question 9

Refer to the figure showing the recognition sequence for EcoRI.   DNA ligase closes the gap between nucleotides that have been cut with restriction enzymes and then reannealed between DNA strands cut with the same restriction enzyme.In the figure, where are the phosphate groups located?

Accepted Answer

In the ade

Question 10

Suppose one restriction enzyme (enzyme A) makes three cuts in a circular DNA molecule, and another restriction enzyme (enzyme B) makes one cut in that same DNA.The cuts are not in the same place, and the cut fragments are not exactly the same size.The restriction digestion is set up on an electrophoresis gel.If only enzyme A is used in the restriction digestion, the number of bands the gel should pick up is _______.

Accepted Answer

The answer of Suppose one restriction enzyme (enzyme A) makes...

Question 11

Hosts that are altered by the insertion of recombinant DNA are known as _______ organisms.

Accepted Answer

The answer of Hosts that are altered by the insertion...

Question 12

A restriction enzyme recognizes the sequence 5ʹ-CTGCAG-3ʹ and makes cuts between G and C.Which statement is most likely true?

Accepted Answer

A)  Sticky ends should arise because the enzyme cuts in the middle of the sequence. 
B)  Sticky ends should arise because the restriction site is less than eight nucleotides long. 
C)  Sticky ends should arise because the restriction site is more than four nucleotides long. 
D)  Blunt ends should arise because the enzyme cuts once in the middle of the sequence. 
E)  Blunt ends should arise because the restriction site is more than four nucleotides long 
A)  Sticky ends should arise because the enzyme cuts in the middle of the sequence. 
B)  Sticky ends should arise because the restriction site is less than eight nucleotides long. 
C)  Sticky ends should arise because the restriction site is more than four nucleotides long. 
D)  Blunt ends should arise because the enzyme cuts once in the middle of the sequence. 
E)  Blunt ends should arise because the restriction site is more than four nucleotides long

Question 13

You are expressing a human gene in yeast cells.Your assistant tells you that the signal sequences used in the experiment are incorrect.What will be the most likely consequence of this error?

Accepted Answer

A)  Too little of the product will be made. 
B)  Too much of the product will be made. 
C)  The product will only be made when the temperature is sufficiently high. 
D)  The product will only be made when the temperature is sufficiently low. 
E)  The product will not go to the appropriate places in the cell. 
A)  Too little of the product will be made. 
B)  Too much of the product will be made. 
C)  The product will only be made when the temperature is sufficiently high. 
D)  The product will only be made when the temperature is sufficiently low. 
E)  The product will not go to the appropriate places in the cell.

Question 14

The sequence 5ʹ-TCACGT-3ʹ is not a restriction enzyme palindromic recognition sequence.What mutational change would most likely make it into one?

Accepted Answer

A)  Changing T to C at the 5ʹ end 
B)  Changing T to A at the 5ʹ end 
C)  Changing T to A at the 3ʹ end 
D)  Changing T to G at the 3ʹ end 
E)  No single point mutation can change this sequence into a recognition sequence. 
A)  Changing T to C at the 5ʹ end 
B)  Changing T to A at the 5ʹ end 
C)  Changing T to A at the 3ʹ end 
D)  Changing T to G at the 3ʹ end 
E)  No single point mutation can change this sequence into a recognition sequence.

Question 15

Found in Agrobacterium tumefaciens, the _______ permits the bacterium to infect plants.It is often used as a vector in plant recombinant DNA technology.

Accepted Answer

The answer of Found in Agrobacterium tumefaciens, the _______ permits...

Question 16

A biologist used microarrays to examine how gene expression in a fish changes with temperature.Refer to the table showing the expression levels of four (of several thousand) genes at varying temperatures.Values for expression have been normalized such that the expression at 15°C for any particular gene is always 100.   The product of gene 1246 is an inhibitor of a pigment that makes fish red.Thus, all other things being equal and assuming that a 25 percent change in expression level is sufficient for a color change, what should be expected?

Accepted Answer

A)  The fish would be the most red at just the highest temperatures. 
B)  The fish would be the most red at just the lowest temperatures. 
C)  The fish would be the most red at intermediate temperatures, and less red at the extremes. 
D)  The fish would be less red at intermediate temperatures, and redder at the extremes. 
E)  Fish color should not vary much with temperature. 
A)  The fish would be the most red at just the highest temperatures. 
B)  The fish would be the most red at just the lowest temperatures. 
C)  The fish would be the most red at intermediate temperatures, and less red at the extremes. 
D)  The fish would be less red at intermediate temperatures, and redder at the extremes. 
E)  Fish color should not vary much with temperature.

Question 17

What is the major difference between using green fluorescent protein and using an antibiotic resistance gene as a reporter gene?

Accepted Answer

A)  The green fluorescent protein can be used only when the host cell is a plant cell because it relies on the process of photosynthesis. 
B)  The antibiotic resistance gene can be used only when the host cell is an animal. 
C)  The green fluorescent protein can be used only in transfection, not in transformation. 
D)  With the green fluorescent protein, no cells are killed in the selection process. 
E)  Homologous recombination will work only with the green fluorescent protein. 
A)  The green fluorescent protein can be used only when the host cell is a plant cell because it relies on the process of photosynthesis. 
B)  The antibiotic resistance gene can be used only when the host cell is an animal. 
C)  The green fluorescent protein can be used only in transfection, not in transformation. 
D)  With the green fluorescent protein, no cells are killed in the selection process. 
E)  Homologous recombination will work only with the green fluorescent protein.

Question 18

Smallest in size of the general classes of vectors, _______ are circular stretches of DNA that are easily manipulated in the laboratory.

Accepted Answer

The answer of Smallest in size of the general classes...

Question 19

Which DNA sequence forms a palindromic sequence?

Accepted Answer

A)  5ʹ-ACTGAG-3ʹ 
B)  5ʹ-ACTATT-3ʹ 
C)  5ʹ-ACTAGT-3ʹ 
D)  5ʹ-ACTACT-3ʹ 
E)  5ʹ-ACTTAT-3ʹ 
A)  5ʹ-ACTGAG-3ʹ 
B)  5ʹ-ACTATT-3ʹ 
C)  5ʹ-ACTAGT-3ʹ 
D)  5ʹ-ACTACT-3ʹ 
E)  5ʹ-ACTTAT-3ʹ

Question 20

Which tool or technique would be most useful in the large-scale commercial production and harvesting of proteins?

Accepted Answer

A)  A genomic library 
B)  Microarray 
C)  miRNA 
D)  RNA interference 
E)  An expression vector 
A)  A genomic library 
B)  Microarray 
C)  miRNA 
D)  RNA interference 
E)  An expression vector

Exam 18: Recombinant Dna and Biotechnology

You are choosing between yeast and bacterial cells as hosts for recombinant DNA.Which statement is most true?

The function of DNA ligase in the generation of recombinant DNA is to _______ DNA.

A lab working with recombinant DNA includes a gene for penicillin resistance as part of the recombinant DNA.In this case, the penicillin resistance gene is acting as a(n) _______ marker.

In the construction of a transgenic cell, a gene that enables growth on arabinose is added to the vector.This gene is being used as a(n)

Which statement concerning a genomic library is true?

Which assay would you most likely use to determine whether the liver, the heart, or the brain of mice is expressing more genes at a given time?

Refer to the figure showing the recognition sequence for EcoRI. DNA ligase closes the gap between nucleotides that have been cut with restriction enzymes and then reannealed between DNA strands cut with the same restriction enzyme.In the figure, where are the phosphate groups located?

Hosts that are altered by the insertion of recombinant DNA are known as _______ organisms.

A restriction enzyme recognizes the sequence 5ʹ-CTGCAG-3ʹ and makes cuts between G and C.Which statement is most likely true?

You are expressing a human gene in yeast cells.Your assistant tells you that the signal sequences used in the experiment are incorrect.What will be the most likely consequence of this error?

The sequence 5ʹ-TCACGT-3ʹ is not a restriction enzyme palindromic recognition sequence.What mutational change would most likely make it into one?

Found in Agrobacterium tumefaciens, the _______ permits the bacterium to infect plants.It is often used as a vector in plant recombinant DNA technology.

What is the major difference between using green fluorescent protein and using an antibiotic resistance gene as a reporter gene?

Smallest in size of the general classes of vectors, _______ are circular stretches of DNA that are easily manipulated in the laboratory.

Which DNA sequence forms a palindromic sequence?

Which tool or technique would be most useful in the large-scale commercial production and harvesting of proteins?

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