Exam 18: Recombinant Dna and Biotechnology

Which technology is used to examine the patterns of gene expression for a large number of genes simultaneously?

A(n) _______ vector contains all the characteristics of cloning vectors, as well as the extra sequences needed for producing the transgene.

Which example would not be considered a form of biotechnology?

Refer to the table showing results from an experiment in which researchers used CRISPR technology to knock out various genes from a fly.They then examined the developmental time (in days, with calculated means) and larval survival (percent, with calculated means) for each treatment.(Note: The "x" marks genes that have been knocked out.) Which gene(s) affect(s) developmental time? Explain your answer.

The sequence 5ʹ-TCATGT-3ʹ is not a restriction enzyme palindromic recognition sequence.What mutational change would most likely make it into one?

A plasmid contains the gene for tetracycline resistance and a lacZ gene that has a restriction site in it.Foreign DNA has the same restriction site at its ends.Host bacteria are transformed with the recombinant DNA and grown on medium with tetracycline and X-Gal.The host bacteria already contained a ciprofloxacin resistance gene prior to the transformation event.Which gene or substance is acting as a reporter gene?

A biologist would most likely use the technique of electroporation to

Which would a biologist generate to get a "snapshot" that captures the transcription pattern of a set of cells at a given point in time?

A cow transgene is to be expressed in a bacterial cell.What else needs to be included in the expression vector?

In an experiment in recombinant DNA, the colonies that contain the correct recombinant DNA have turned blue.The most likely selective marker being used is

Why might cultured plant cells be more useful than animal cells as host cells, even for animal transgenes?

In comparison with selective breeding, recombinant DNA technology

Recombinant DNA involves the joining of two distinct DNA molecules.The enzyme that catalyzes this reaction is known as _______.

DNA microarrays

Which feature is an advantage that viruses (and phages) have over plasmids, with respect to their ability to be vectors?

A biologist used microarrays to examine how gene expression in a fish changes with temperature.Refer to the table showing the expression levels of four (of several thousand) genes at varying temperatures.Values for expression have been normalized such that the expression at 15°C for any particular gene is always 100. The expression of gene(s) _______ steadily increase(s) as temperature increases.

cDNA and genomic libraries are created from a mouse at different times in development.You would expect the cDNA libraries taken at different times to have _______ information and be _______ variable than genomic libraries taken from the same samples.

Which molecule is recombinant DNA?

Suppose you are a researcher who is expressing a human transgene in yeast cells.You want to be able to easily control when the gene is expressed.Which tool or technique will give you that type of control?

Refer to the figure showing the steps used in microarray analysis to detect differences in expression between tumor tissue and normal tissue.The spots in the bottom panel represent individual genes for patients who have good prognoses (left) as well as for patients who have poor ones (right).The colors of those spots represent levels of expression: red = high expression, yellow = intermediate expression, green = low expression. Look at the top leftmost spot.At this gene, patients with poor prognoses have _______ expression than patients with good prognoses.