Question 1

After electrophoresis has been performed, the stripes in one lane of a gel are

Accepted Answer

A)  different-sized DNA molecules. 
B)  the digested restriction enzyme. 
C)  RNA from different cells. 
D)  DNA hybridization sites. 
A)  different-sized DNA molecules. 
B)  the digested restriction enzyme. 
C)  RNA from different cells. 
D)  DNA hybridization sites.

Question 2

Among these DNA fragments which would move most quickly during gel electrophoresis?

Accepted Answer

A)  AATAAT 
B)  AATAATAATAAT 
C)  AAATTAAT 
D)  AATAATAATAATAATAATAAT 
A)  AATAAT 
B)  AATAATAATAAT 
C)  AAATTAAT 
D)  AATAATAATAATAATAATAAT

Question 3

If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene?

Accepted Answer

A)  treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis 
B)  insert the known gene into a vector and use the vector to insert the known gene into the other organism 
C)  create labeled DNA probes from the known gene and use them to screen the DNA library of the other organism 
D)  create a hybrid of the two organisms by breeding them and check for mutations 
A)  treat the genomes of both organisms with the same restriction enzyme and compare the patterns of the bands produced with gel electrophoresis 
B)  insert the known gene into a vector and use the vector to insert the known gene into the other organism 
C)  create labeled DNA probes from the known gene and use them to screen the DNA library of the other organism 
D)  create a hybrid of the two organisms by breeding them and check for mutations

Question 4

Which of the following DNA fragments would bind to the short probe, AAG?

Accepted Answer

A)  ATACCAGGC 
B)  AAATGTATG 
C)  AAATTCATG 
D)  TTAGGTTTG 
A)  ATACCAGGC 
B)  AAATGTATG 
C)  AAATTCATG 
D)  TTAGGTTTG

Question 5

DNA _______ is the base pairing of DNA from two different sources.

Accepted Answer

The answer of DNA _______ is the base pairing of...

Question 6

A segment of DNA in a test tube replicates and produces many copies of itself. What was probably in the test tube that enhanced this process?

Accepted Answer

A)  a ligase 
B)  a restriction enzyme 
C)  DNA polymerase 
D)  an electric current 
A)  a ligase 
B)  a restriction enzyme 
C)  DNA polymerase 
D)  an electric current

Question 7

DNA moves toward the __________ charged end of an electrical field during gel electrophoresis.

Accepted Answer

The answer of DNA moves toward the __________ charged end...

Question 8

Gel electrophoresis uses

Accepted Answer

A)  an electrical current. 
B)  ligases. 
C)  DNA polymerase. 
D)  viral vectors. 
A)  an electrical current. 
B)  ligases. 
C)  DNA polymerase. 
D)  viral vectors.

Question 9

DNA _______ is the set of techniques with which scientists manipulate DNA.

Accepted Answer

The answer of DNA _______ is the set of techniques...

Question 10

Human gene therapy involves

Accepted Answer

A)  correcting genetic disorders by modifying the genes that cause them. 
B)  using plasmids to carry genes that will break down a specific harmful gene in a patient. 
C)  inserting genes from other organisms into the human genome. 
D)  using PCR to produce large amounts of normal DNA that can be injected into a patient. 
A)  correcting genetic disorders by modifying the genes that cause them. 
B)  using plasmids to carry genes that will break down a specific harmful gene in a patient. 
C)  inserting genes from other organisms into the human genome. 
D)  using PCR to produce large amounts of normal DNA that can be injected into a patient.

Question 11

Growing a new house plant from a cutting taken from another plant is an example of whole organism cloning.

Accepted Answer

A) True 
 B)False

Question 12

During _______________ cloning, the nucleus of an egg cell is replaced with the nucleus of a somatic cell from the individual being cloned.

Accepted Answer

The answer of During _______________ cloning, the nucleus of an...

Question 13

A _______ is a small, circular portion of DNA that is found in bacteria and is not part of the main genome.

Accepted Answer

The answer of A _______ is a small, circular portion...

Question 14

Many different fragments of DNA from an oak tree genome are inserted into a plasmid vector, which is then taken up by bacteria that copy the inserted DNA every time they reproduce. This procedure is known as

Accepted Answer

A)  DNA probing. 
B)  DNA fingerprinting. 
C)  the polymerase chain reaction (PCR). 
D)  the construction of a DNA library. 
A)  DNA probing. 
B)  DNA fingerprinting. 
C)  the polymerase chain reaction (PCR). 
D)  the construction of a DNA library.

Question 15

Genetic engineering works because all organisms

Accepted Answer

A)  contain plasmids. 
B)  can contract the same diseases. 
C)  share the same chemical DNA structure. 
D)  contain the same genes. 
A)  contain plasmids. 
B)  can contract the same diseases. 
C)  share the same chemical DNA structure. 
D)  contain the same genes.

Question 16

DNA fingerprinting can prove guilt without fail.

Accepted Answer

A) True 
 B)False

Question 17

Human gene _______ seeks to correct genetic disorders by fixing the genes that cause the disorders.

Accepted Answer

The answer of Human gene _______ seeks to correct genetic...

Question 18

The figure below shows the first cycle in a polymerase chain reaction (PCR).  
What is the role of the structure marked "P" in this figure?

Accepted Answer

A)  The "P" represents the heat-tolerant DNA polymerase used in PCR to copy the gene of interest. 
B)  The "P" represents a sequence of DNA complementary to parts of the gene being amplified that allows DNA polymerase to attach to the gene. 
C)  The "P" indicates where on the DNA that DNA polymerase should terminate replication. 
D)  The "P" indicates the location of the promoter for the gene being amplified. 
A)  The "P" represents the heat-tolerant DNA polymerase used in PCR to copy the gene of interest. 
B)  The "P" represents a sequence of DNA complementary to parts of the gene being amplified that allows DNA polymerase to attach to the gene. 
C)  The "P" indicates where on the DNA that DNA polymerase should terminate replication. 
D)  The "P" indicates the location of the promoter for the gene being amplified.

Question 19

Genetic engineering is a process in which specific sequences of DNA _______ are deliberately isolated, modified, and inserted back into a host organism.

Accepted Answer

The answer of Genetic engineering is a process in which...

Question 20

The goal of DNA __________ is to make many copies of a piece of recombinant DNA so that the DNA can be further manipulated and studied.

Accepted Answer

The answer of The goal of DNA __________ is to...

Exam 15: DNA Technology

After electrophoresis has been performed, the stripes in one lane of a gel are

Among these DNA fragments which would move most quickly during gel electrophoresis?

If you knew the sequence of a gene in one organism, how could you determine if another organism had a similar gene?

Which of the following DNA fragments would bind to the short probe, AAG?

DNA _______ is the base pairing of DNA from two different sources.

A segment of DNA in a test tube replicates and produces many copies of itself. What was probably in the test tube that enhanced this process?

DNA moves toward the __________ charged end of an electrical field during gel electrophoresis.

Gel electrophoresis uses

DNA _______ is the set of techniques with which scientists manipulate DNA.

Human gene therapy involves

Growing a new house plant from a cutting taken from another plant is an example of whole organism cloning.

During _______________ cloning, the nucleus of an egg cell is replaced with the nucleus of a somatic cell from the individual being cloned.

A _______ is a small, circular portion of DNA that is found in bacteria and is not part of the main genome.

Many different fragments of DNA from an oak tree genome are inserted into a plasmid vector, which is then taken up by bacteria that copy the inserted DNA every time they reproduce. This procedure is known as

Genetic engineering works because all organisms

DNA fingerprinting can prove guilt without fail.

Human gene _______ seeks to correct genetic disorders by fixing the genes that cause the disorders.

The figure below shows the first cycle in a polymerase chain reaction (PCR). What is the role of the structure marked "P" in this figure?

Genetic engineering is a process in which specific sequences of DNA _______ are deliberately isolated, modified, and inserted back into a host organism.

The goal of DNA __________ is to make many copies of a piece of recombinant DNA so that the DNA can be further manipulated and studied.

The Nature of Science and the Characteristics of Life

Organizing the Diversity of Life

Major Groups of Living Organisms

Chemical Building Blocks

Cell Structure and Internal Compartments

Cell Membranes, Transport, and Communication

Energy and Enzymes

Photosynthesis and Cellular Respiration

Cell Division

Patterns of Inheritance

Chromosomes and Human Genetics

DNA

From Gene to Protein

Control of Gene Expression

How Evolution Works

Evolution of Populations

Adaptation and Speciation

The Evolutionary History of Life

Maintaining the Internal Environment

Animal Nutrition and Digestion

Gas Exchange

The Circulatory System

Animal Hormones

The Nervous System

Sensing the Environment

Skeletons, Muscles, and Movement

Defenses against Disease

Reproduction and Development

Animal Behavior

Plant Structure, Nutrition, and Transport

Plant Growth and Reproduction

The Biosphere

Growth of Populations

Interactions among Organisms

Communities of Organisms

Ecosystems

Global Change

Biodiversity and People

Cancer: Cell Division Out of Control

Harnessing the Human Genome

Humans and Evolution

Smoking--Beyond Lung Cancer

Feeding a Hungry Planet

Building a Sustainable Society

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