Question 1

The use of DNA analysis to identify individuals is known as

Accepted Answer

A)  PCR testing. 
B)  DNA fingerprinting. 
C)  genetic modification. 
D)  cloning. 
A)  PCR testing. 
B)  DNA fingerprinting. 
C)  genetic modification. 
D)  cloning.

Question 2

It is theoretically impossible for two individuals (other than identical twins) to have the same DNA fingerprint

Accepted Answer

A) True 
 B)False

Question 3

In research, restriction enzymes are used to

Accepted Answer

A)  stop replication of DNA at a specific base during DNA sequencing. 
B)  cut DNA molecules into pieces that can be used to create recombinant DNA. 
C)  measure the relative amounts of each base in a DNA molecule. 
D)  join two fragments of DNA together to form a molecule of recombinant DNA. 
A)  stop replication of DNA at a specific base during DNA sequencing. 
B)  cut DNA molecules into pieces that can be used to create recombinant DNA. 
C)  measure the relative amounts of each base in a DNA molecule. 
D)  join two fragments of DNA together to form a molecule of recombinant DNA.

Question 4

HindIII and AluI are examples of _______ _______ used to cut DNA at specific sites.

Accepted Answer

The answer of HindIII and AluI are examples of _______...

Question 5

Genetic engineering works only on animals and has not yet been successfully used on plants.

Accepted Answer

A) True 
 B)False

Question 6

DNA extraction

Accepted Answer

A)  uses restriction enzymes to prevent chromosomes from unraveling. 
B)  can only occur when the gene of interested has be amplified by PCR. 
C)  occurs only in prokaryotic cells since they lack a protective nucleus. 
D)  requires that all non-DNA organic compounds be removed with chemicals. 
A)  uses restriction enzymes to prevent chromosomes from unraveling. 
B)  can only occur when the gene of interested has be amplified by PCR. 
C)  occurs only in prokaryotic cells since they lack a protective nucleus. 
D)  requires that all non-DNA organic compounds be removed with chemicals.

Question 7

DNA polymerase is used in the laboratory to

Accepted Answer

A)  perform gel electrophoresis procedures. 
B)  cure patients of certain genetic diseases. 
C)  make many copies of a DNA sequence. 
D)  attach DNA fragments to each other. 
A)  perform gel electrophoresis procedures. 
B)  cure patients of certain genetic diseases. 
C)  make many copies of a DNA sequence. 
D)  attach DNA fragments to each other.

Question 8

In the first step of the polymerase chain reaction (PCR), the two strands of the DNA being amplified must be separated by exposing the DNA to high _______.

Accepted Answer

heat or te

Question 9

A vector is used to

Accepted Answer

A)  transfer a fragment of DNA from one species to another. 
B)  separate DNA fragments from one another. 
C)  probe DNA for faulty genes. 
D)  attach DNA fragments to one another. 
A)  transfer a fragment of DNA from one species to another. 
B)  separate DNA fragments from one another. 
C)  probe DNA for faulty genes. 
D)  attach DNA fragments to one another.

Question 10

The polymerase chain reaction (PCR) uses a _______ that can pair to the target DNA to give DNA polymerase a starting point.

Accepted Answer

The answer of The polymerase chain reaction (PCR) uses a...

Question 11

To make recombinant DNA,

Accepted Answer

A)  two nonhomologous chromosomes are induced to undergo crossing-over during meiosis. 
B)  the entire genome of one organism is fused to a plasmid. 
C)  pieces of DNA from different organisms are cut with restriction enzymes and then glued together with ligase. 
D)  DNA polymerase is used to make multiple copies of the DNA sequences of two organisms at the same time. 
A)  two nonhomologous chromosomes are induced to undergo crossing-over during meiosis. 
B)  the entire genome of one organism is fused to a plasmid. 
C)  pieces of DNA from different organisms are cut with restriction enzymes and then glued together with ligase. 
D)  DNA polymerase is used to make multiple copies of the DNA sequences of two organisms at the same time.

Question 12

A detective finds a miniscule spot of blood on a murder suspect's shirt. What method would she expect the police lab to use to get enough DNA from the blood droplet to perform DNA fingerprinting?

Accepted Answer

A)  inserting the blood's DNA into viral DNA 
B)  polymerase chain reaction (PCR) 
C)  inserting the sample's DNA into a plasmid 
D)  using ligases to bond the DNA fragments together for sequencing. 
A)  inserting the blood's DNA into viral DNA 
B)  polymerase chain reaction (PCR) 
C)  inserting the sample's DNA into a plasmid 
D)  using ligases to bond the DNA fragments together for sequencing.

Question 13

There are no risks associated with DNA technology.

Accepted Answer

A) True 
 B)False

Question 14

_______ catalyzes the formation of covalent bonds between two DNA fragments, creating a single piece of DNA.

Accepted Answer

The answer of _______ catalyzes the formation of covalent bonds...

Question 15

DNA identification of individuals is possible because

Accepted Answer

A)  the same restriction enzyme cuts different individuals' DNA in different places. 
B)  a different restriction enzyme is needed to cut each person's DNA. 
C)  a different species of bacteria is needed for the DNA library of each person. 
D)  each person's DNA uses a different set of bases. 
A)  the same restriction enzyme cuts different individuals' DNA in different places. 
B)  a different restriction enzyme is needed to cut each person's DNA. 
C)  a different species of bacteria is needed for the DNA library of each person. 
D)  each person's DNA uses a different set of bases.

Question 16

RFLP analysis is one method used to perform DNA fingerprinting.

Accepted Answer

A) True 
 B)False

Question 17

A sample of the same DNA is placed into each of two test tubes. Each tube is treated with a restriction enzyme and the resulting fragments are separated by gel electrophoresis producing the gel shown in the figure below.  
Which of the following explains why the pattern of DNA fragments differs in these two samples?

Accepted Answer

A)  Different restriction enzymes must have been used in the different tubes. 
B)  One tube was heated, but the other tube was not. 
C)  Ligase was added to one of the tubes, but not the other. 
D)  The restriction enzyme added to the tubes cut DNA at more than one sequence. 
A)  Different restriction enzymes must have been used in the different tubes. 
B)  One tube was heated, but the other tube was not. 
C)  Ligase was added to one of the tubes, but not the other. 
D)  The restriction enzyme added to the tubes cut DNA at more than one sequence.

Question 18

The polymerase chain reaction (PCR) is used to

Accepted Answer

A)  produce many copies of a selected DNA sequence. 
B)  insert DNA from one organism into a new host. 
C)  screen for a particular gene. 
D)  deliver DNA products into a human patient. 
A)  produce many copies of a selected DNA sequence. 
B)  insert DNA from one organism into a new host. 
C)  screen for a particular gene. 
D)  deliver DNA products into a human patient.

Question 19

In nature, the function of a restriction enzyme is to

Accepted Answer

A)  connect DNA fragments after replication. 
B)  protect bacteria from invading foreign DNA. 
C)  speed up the rate at which bacteria can metabolize their energy sources. 
D)  make numerous copies of a given gene. 
A)  connect DNA fragments after replication. 
B)  protect bacteria from invading foreign DNA. 
C)  speed up the rate at which bacteria can metabolize their energy sources. 
D)  make numerous copies of a given gene.

Question 20

The primers used in PCR are

Accepted Answer

A)  identical to the entire base sequence of one strand of the DNA. 
B)  produced when a gene of interest is read by restriction enzymes. 
C)  attached to the gene of interest by ligase. 
D)  complementary to DNA sequences at both ends of the gene of interest. 
A)  identical to the entire base sequence of one strand of the DNA. 
B)  produced when a gene of interest is read by restriction enzymes. 
C)  attached to the gene of interest by ligase. 
D)  complementary to DNA sequences at both ends of the gene of interest.

Exam 15: DNA Technology

The use of DNA analysis to identify individuals is known as

It is theoretically impossible for two individuals (other than identical twins) to have the same DNA fingerprint

In research, restriction enzymes are used to

HindIII and AluI are examples of _______ _______ used to cut DNA at specific sites.

Genetic engineering works only on animals and has not yet been successfully used on plants.

DNA extraction

DNA polymerase is used in the laboratory to

In the first step of the polymerase chain reaction (PCR), the two strands of the DNA being amplified must be separated by exposing the DNA to high _______.

A vector is used to

The polymerase chain reaction (PCR) uses a _______ that can pair to the target DNA to give DNA polymerase a starting point.

To make recombinant DNA,

A detective finds a miniscule spot of blood on a murder suspect's shirt. What method would she expect the police lab to use to get enough DNA from the blood droplet to perform DNA fingerprinting?

There are no risks associated with DNA technology.

_______ catalyzes the formation of covalent bonds between two DNA fragments, creating a single piece of DNA.

DNA identification of individuals is possible because

RFLP analysis is one method used to perform DNA fingerprinting.

The polymerase chain reaction (PCR) is used to

In nature, the function of a restriction enzyme is to

The primers used in PCR are

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HindIII and AluI are examples of _ _ used to cut DNA at specific sites.