Exam 20: Biotechnology

DNA fragments from a gel are transferred to a nitrocellulose paper during the procedure called Southern blotting. What is the purpose of transferring the DNA from a gel to a nitrocellulose paper?

Which of the following cuts DNA molecules at specific locations?

In 1997, Dolly the sheep was cloned. Which of the following processes was used?

Use the following information to answer the following questions. CML (chronic myelogenous leukemia) results from a translocation between human chromosomes 9 and 22. The resulting chromosome 22 is significantly shorter than the usual, and it is known as a Philadelphia (Ph') chromosome. The junction at the site of the translocation causes over-expression of a thymine kinase receptor. A new drug (Gleevec or imatinib) has been found to inhibit the disease if the patient is treated early. -As genetic technology makes testing for a wide variety of genotypes possible, which of the following is likely to be an increasingly troublesome issue?

Use Figure 20.3 to answer the following questions. The DNA profiles below represent four different individuals. Figure 20.3 -Which of the following statements is most likely True?

A researcher is using adult stem cells and comparing them to other adult cells from the same tissue. Which of the following is a likely finding?

Which uses reverse transcriptase to make cDNA followed by amplification?

Which describes the transfer of polypeptide sequences to a membrane to analyze gene expression?

DNA technology has many medical applications. Which of the following is not done routinely at present?

Use the following information to answer the questions below. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics. -Bacteria that do not take up any plasmids would grow on which media?

DNA microarrays have made a huge impact on genomic studies because they

Which was developed by a British researcher and causes DNA sequences to be transferred to a membrane and identified with a probe?

Which of the following would not be True of cDNA produced using human brain tissue as the starting material?

Dideoxyribonucleotide chain-termination is a method of

Which of the following best describes the complete sequence of steps occurring during every cycle of PCR? 1) The primers hybridize to the target DNA. 2) The mixture is heated to a high temperature to denature the double stranded target DNA. 3) Fresh DNA polymerase is added. 4) DNA polymerase extends the primers to make a copy of the target DNA.

Figure 20.2 -The segment of DNA shown in Figure 20.2 has restriction sites I and II, which create restriction fragments A, B, and C. Which of the gels produced by electrophoresis shown below best represents the separation and identity of these fragments?

Use Figure 20.3 to answer the following questions. The DNA profiles below represent four different individuals. Figure 20.3 -Genetically engineered plants

Use the following information to answer the questions below. A eukaryotic gene has "sticky ends" produced by the restriction endonuclease EcoRI. The gene is added to a mixture containing EcoRI and a bacterial plasmid that carries two genes conferring resistance to ampicillin and tetracycline. The plasmid has one recognition site for EcoRI located in the tetracycline resistance gene. This mixture is incubated for several hours, exposed to DNA ligase, and then added to bacteria growing in nutrient broth. The bacteria are allowed to grow overnight and are streaked on a plate using a technique that produces isolated colonies that are clones of the original. Samples of these colonies are then grown in four different media: nutrient broth plus ampicillin, nutrient broth plus tetracycline, nutrient broth plus ampicillin and tetracycline, and nutrient broth without antibiotics. -Bacteria containing a plasmid into which the eukaryotic gene has integrated would grow in

Use the following information to answer the following questions. CML (chronic myelogenous leukemia) results from a translocation between human chromosomes 9 and 22. The resulting chromosome 22 is significantly shorter than the usual, and it is known as a Philadelphia (Ph') chromosome. The junction at the site of the translocation causes over-expression of a thymine kinase receptor. A new drug (Gleevec or imatinib) has been found to inhibit the disease if the patient is treated early. -Which of the following tools of recombinant DNA technology is incorrectly paired with its use?

One successful form of gene therapy has involved delivery of an allele for the enzyme adenosine deaminase (ADA)to bone marrow cells of a child with SCID, and delivery of these engineered cells back to the bone marrow of the affected child. What is one major reason for the success of this procedure as opposed to many other efforts at gene therapy?