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Use the Following Information to Answer the Following Questions

Question 59

Multiple Choice

Use the following information to answer the following questions.
CML (chronic myelogenous leukemia) results from a translocation between human chromosomes 9 and 22. The resulting chromosome 22 is significantly shorter than the usual, and it is known as a Philadelphia (Ph') chromosome. The junction at the site of the translocation causes over-expression of a thymine kinase receptor. A new drug (Gleevec or imatinib) has been found to inhibit the disease if the patient is treated early.
-Which of the following tools of recombinant DNA technology is incorrectly paired with its use?


A) restriction enzyme-production of RFLPs
B) DNA ligase-enzyme that cuts DNA, creating the sticky ends of restriction fragments
C) DNA polymerase-used in a polymerase chain reaction to amplify sections of DNA
D) reverse transcriptase-production of cDNA from mRNA
E) electrophoresis-separation of DNA fragments

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