Exam 20: Biotechnology

Use the following information to answer the following questions. CML (chronic myelogenous leukemia) results from a translocation between human chromosomes 9 and 22. The resulting chromosome 22 is significantly shorter than the usual, and it is known as a Philadelphia (Ph') chromosome. The junction at the site of the translocation causes over-expression of a thymine kinase receptor. A new drug (Gleevec or imatinib) has been found to inhibit the disease if the patient is treated early. -Why would Gleevec most probably cause remission of the disease?

Expression of a cloned eukaryotic gene in a bacterial cell involves many challenges. The use of mRNA and reverse transcriptase is part of a strategy to solve the problem of

A gene that contains introns can be made shorter (but remain functional)for genetic engineering purposes by using

Which of the following is one of the technical reasons why gene therapy is problematic?

Use the following information to answer the following questions. CML (chronic myelogenous leukemia) results from a translocation between human chromosomes 9 and 22. The resulting chromosome 22 is significantly shorter than the usual, and it is known as a Philadelphia (Ph') chromosome. The junction at the site of the translocation causes over-expression of a thymine kinase receptor. A new drug (Gleevec or imatinib) has been found to inhibit the disease if the patient is treated early. -Which of the following would be a reasonably efficient technique for confirming the diagnosis of CML?

Which of the following techniques used to analyze gene function depends on the specificity of DNA base complementarity?

The DNA fragments making up a genomic library are generally contained in

Use Figure 20.3 to answer the following questions. The DNA profiles below represent four different individuals. Figure 20.3 -Which of the following statements is consistent with the results?

Reproductive cloning of human embryos is generally considered unethical. However, on the subject of therapeutic cloning there is a wider divergence of opinion. Which of the following is a likely explanation?

Which of the following sequences in double-stranded DNA is most likely to be recognized as a cutting site for a restriction enzyme?

Use Figure 20.3 to answer the following questions. The DNA profiles below represent four different individuals. Figure 20.3 -Genetic engineering is being used by the pharmaceutical industry. Which of the following is not currently one of the uses?

Which of the following problems with animal cloning might result in premature death of the clones?

Which of the following produces multiple identical copies of a gene for basic research or for large-scale production of a gene product?

In order to identify a specific restriction fragment using a probe, what must be done?

Which of the following is True of embryonic stem cells but not of adult stem cells?

Use Figure 20.3 to answer the following questions. The DNA profiles below represent four different individuals. Figure 20.3 -Which of the following are probably siblings?

How does a genomic library differ from a cDNA library?

A researcher needs to clone a sequence of part of a eukaryotic genome in order to express the sequence and to modify the polypeptide product. She would be able to satisfy these requirements by using which of the following vectors?

Which of the following is most closely identical to the formation of twins?

In recombinant DNA methods, the term vector can refer to