Exam 17: From Gene to Protein

An experimenter has altered the 3' end of the tRNA corresponding to the amino acid methionine in such a way as to remove the 3' AC.Which of the following hypotheses describes the most likely result?

What is the mechanism of information transfer from DNA to protein synthesis in eukaryotes?

How might a single base substitution in the sequence of a gene affect the amino acid sequence of a protein encoded by the gene,and why?

Use the following information to answer the next few questions. A transfer RNA (#1)attached to the amino acid lysine enters the ribosome.The lysine binds to the growing polypeptide on the other tRNA (#2)in the ribosome already. -Which component of the complex described enters the exit tunnel through the large subunit of the ribosome?

Which of the following nucleotide triplets best represents a codon?

The term polyribosome refers to

Which of the following does not occur in prokaryotic gene expression,but does in eukaryotic gene expression?

Rank the following one-base point mutations (from most likely to least likely)with respect to their likelihood of affecting the structure of the corresponding polypeptide. 1)insertion mutation within an intron 2)substitution mutation at the third position of an exonic codon 3)substitution mutation at the second position of an exonic codon 4)deletion mutation within the first exon of the gene

What are the correct sizes of the following? 1)A primary transcript in the nucleus of a eukaryotic cell compared to the functional mRNA 2)A primary transcript in a prokaryotic cell compared to the functional mRNA

What is the function of GTP in translation?

What is the function of the release factor (RF)?

Use the following figure to answer the questions below. -What type of bonding is responsible for maintaining the shape of the tRNA molecule?

Use the following information to answer the questions below. Yeast is capable of synthesizing the amino acids necessary for survival and can grow on minimal media or enriched media easily.In both conditions,the colonies have a cream colour.The production of adenine is regulated by two genes,ADE1 and ADE2.If either of these genes is defective,adenine cannot be synthesized and the colony will look red (as a result of accumulation of precursors).A common undergraduate lab study is to irradiate yeast cells with ultra violet light from 2-5 sec up to 1-5 minutes,and see what will happen. -If,after irradiating and growing cultures in enriched media,you see some red colonies,what is the easiest way to confirm that the phenotype observed is due to a lack of adenine metabolism?

In the structural organization of many eukaryotic genes,individual exons may be related to which of the following?

Which of the following is a useful feature of introns?

The following questions refer to this table of codons. -If a nucleotide substitution switched an A for a U,such that the codon was read as UAA instead of AAA,what would the effect be?

Suppose that an induced mutation removes most of the 5' end of the 5' UTR.What might result?

Post-translational modifications of proteins may include which of the following?

Garrod hypothesized that "inborn errors of metabolism" such as alkaptonuria occur because

The nitrogenous base adenine is found in all members of which group?