Exam 36: Flow Cytometry

A patient blood sample suspected of having ALL is received in the flow lab for immunophenotyping.The analysis is performed, and standard B cell markers are present, but the patient is also CD33-positive.What is the most likely reason for this occurrence?

Which of the following is/are clinical application(s) of flow cytometry?

Which of the following is/are component(s) of a flow cytometer?

A flow instrument printout reveals a single scatter population of small size and low complexity.Ninety percent of the cells in that population have CD2 on their surface.What is the most probable lineage of the unknown population?

Immunophenotyping is essential for the diagnosis of ALL, separation of T and B lineage ALL, and identification of subtypes of B lineage ALL.What markers can blasts express in precursor B cell ALL?

The percentage of CD4 positive lymphocytes is determined by flow cytometry using staining with fluorescent-tagged antibodies against CD3 and CD4, followed by whole blood RBC lysis.The dual platform uses what calculation to determine the absolute number of CD4 positive cells?

The gold standard for the diagnosis of AML is based on what information?

Choose the accurate statement(s) regarding fluorochromes from the choices below.

An HIV-positive patient has his blood drawn to get his CD4 count.His results are as follows: WBC count: 2.0 x 109/L with 15% lymphocytes.CD4 positive cells are 10% of the total lymphocyte count.What is this patient's absolute CD4 count, and what does this mean for the patient?

Which markers are useful in immunophenotyping for leukemia/lymphoma of T cell lineage?

Which of the following is used to standardize the procedure in flow cytometry?

Which of the following is used to determine the relationship between the content of tumor cells and normal dividing cells?

Explain why flow cytometry is of limited use in identifying AML.

The expression of several antigens from two different lineages complicates the placement of the diagnosis into categories of AML and ALL.This biphenotypic expression toward more than one lineage restricts the use of immunophenotyping.In differentiating AML and ALL, staining with which of the following cytochemical stains indicates AML?

An increased IRF is seen in which of the following?