Question 1

What is the main difference between the methodologies employed on the Coulter Gen-S and the Coulter LH 780?

Accepted Answer

A) Hemoglobin detection using cyanide 
B) Sphering RBCs in retic measurement 
C) Histograms in Gen-S and cytograms in LH 780 
D) Ability to report the NRBC percentage and 
A) Hemoglobin detection using cyanide 
B) Sphering RBCs in retic measurement 
C) Histograms in Gen-S and cytograms in LH 780 
D) Ability to report the NRBC percentage and D

Question 2

Which instrument does NOT use fluorescence intensity in its determination of reticulocytes?

Accepted Answer

A)  LH 750 
B)  XE-2100 
C)  ADVIA 120 
D)  CELL-DYN Sapphire 
A)  LH 750 
B)  XE-2100 
C)  ADVIA 120 
D)  CELL-DYN Sapphire A

Question 3

Explain the purpose of the following used in the standard hematologic cell counting detection methods:
A)VCS flow cell
B)Photodetector
C)Sheath fluid
D)Direct current detection block

Accepted Answer

VCS flow cell: to detect each WBC's volume, conductivity, and scatter for determination of the five part differential.
Photodetector: to measure the absorbance of the unknown blood sample passing through it used in spectrophotometric detection of hgb)
Sheath fluid: Promotes hydrodynamic focusing of cells passing through photodetector.
Direct current detection block: to detect electrical resistance when cells pass through the aperture

Question 4

The principle that relies on detecting resistance as cells pass through a photo-detector and is that used by most of the major hematology analyzers is:

Accepted Answer

A) Spectrophotometry. 
B) Impedance. 
C) Radio frequency. 
D) MAPSS technology. 
A) Spectrophotometry. 
B) Impedance. 
C) Radio frequency. 
D) MAPSS technology.

Question 5

Which technologies are used to generate the LH 750's WBC scatterplot?

Accepted Answer

A)  Impedance, light scatter, and conductivity 
B)  Fluorescence intensity and radio frequency 
C)  Absorbance and conductivity 
D)  Light scatter, absorbance, and radio frequency 
A)  Impedance, light scatter, and conductivity 
B)  Fluorescence intensity and radio frequency 
C)  Absorbance and conductivity 
D)  Light scatter, absorbance, and radio frequency

Question 6

Which parameter does the Coulter LH 750 instrument derive from a histogram?

Accepted Answer

A)  Platelet count 
B)  MCH 
C)  Leukocyte count 
D)  Hct 
A)  Platelet count 
B)  MCH 
C)  Leukocyte count 
D)  Hct

Question 7

Using the chromogenic methodology, a coagulation protein's activity level is related to:

Accepted Answer

A)  An increase in turbidity. 
B)  The observed color change. 
C)  Time needed to convert fibrinogen to fibrin. 
D)  Decreased viscosity as plasmin digests fibrin. 
A)  An increase in turbidity. 
B)  The observed color change. 
C)  Time needed to convert fibrinogen to fibrin. 
D)  Decreased viscosity as plasmin digests fibrin.

Question 8

Using the Fibrometer instrument, clot detection occurs when:

Accepted Answer

A)  Fibrin strands complete an electric circuit. 
B)  Fibrin formation increases light absorbance. 
C)  Fibrin strands decrease ball movement in a magnetic field. 
D)  Fibrin formation decreases turbidity of the reaction mixture. 
A)  Fibrin strands complete an electric circuit. 
B)  Fibrin formation increases light absorbance. 
C)  Fibrin strands decrease ball movement in a magnetic field. 
D)  Fibrin formation decreases turbidity of the reaction mixture.

Question 9

What is the principle of impedance? Name at least two instruments that employ this method for blood cell counting.

Accepted Answer

Impedance relies on the principle of inc

Question 10

In the reticulocyte determination, using the Sysmex XE-2100, the fluorescent dye containing oxazine and polymethine penetrates the cell membranes and stains the blood cells.What does the oxazine bind to within the cells?

Accepted Answer

A)  Residual DNA 
B)  Hemoglobin 
C)  Residual RNA 
D)  Mitochondria 
A)  Residual DNA 
B)  Hemoglobin 
C)  Residual RNA 
D)  Mitochondria

Question 11

The immunologic technique available on the AMAX Destiny Plus utilizes specific 	reagents with:

Accepted Answer

A)  Soluble monoclonal antibody. 
B)  Fluorescent-labeled microparticles. 
C)  Enzyme-labeled monoclonal antibody. 
D)  Monoclonal antibody-coated microparticles. 
A)  Soluble monoclonal antibody. 
B)  Fluorescent-labeled microparticles. 
C)  Enzyme-labeled monoclonal antibody. 
D)  Monoclonal antibody-coated microparticles.

Question 12

What is the purpose of the photodetector on the Diagnostica Stago STA Compact?

Accepted Answer

A) To measure an increase in absorbance caused by the interaction of VWF with its monoclonal antibody 
B) To measure an increase in transmittance caused by the interaction of D-dimer fragments in the patient's plasma with its monoclonal antibody 
C) To promote the ball's oscillation movement through the electromagnetic sensors 
D) To measure the amount of cleavage of p-nitroaniline 
A) To measure an increase in absorbance caused by the interaction of VWF with its monoclonal antibody 
B) To measure an increase in transmittance caused by the interaction of D-dimer fragments in the patient's plasma with its monoclonal antibody 
C) To promote the ball's oscillation movement through the electromagnetic sensors 
D) To measure the amount of cleavage of p-nitroaniline

Question 13

Regarding the CELL-DYN Sapphire, which parameter is determined by the impedance principle?

Accepted Answer

A)  Hemoglobin 
B)  Nucleated erythrocyte count 
C)  Platelet count 
D)  Total leukocyte count 
A)  Hemoglobin 
B)  Nucleated erythrocyte count 
C)  Platelet count 
D)  Total leukocyte count

Question 14

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?

Accepted Answer

A) 0 degrees 
B) 7 degrees 
C) 90 degrees 
D) 90 degrees depolarized 
A) 0 degrees 
B) 7 degrees 
C) 90 degrees 
D) 90 degrees depolarized

Question 15

Compare and contrast the methods of detection for the following analyzers:
A)Sysmex XE-2100
B)Abbott Cell-Dyn Sapphire
C)Coulter LH 780
D)Advia 2120

Accepted Answer

The answer of Compare and contrast the methods of detection...

Question 16

Which analyzer measures platelets in the same channel as reticulocytes?

Accepted Answer

A) Coulter Gen-S 
B) Sysmex XE-2100 
C) Advia 2120 
D) Trinity Biotech AMAX Destiny Plus 
A) Coulter Gen-S 
B) Sysmex XE-2100 
C) Advia 2120 
D) Trinity Biotech AMAX Destiny Plus

Question 17

Which of the following parameters are calculated on the Coulter Gen-S?

Accepted Answer

A) Hemoglobin, MCH, MCHC 
B) Hematocrit, MCH, MCHC 
C) Absolute neutrophil count, RBC count, platelet count 
D) RBC count, hematocrit, MCH 
A) Hemoglobin, MCH, MCHC 
B) Hematocrit, MCH, MCHC 
C) Absolute neutrophil count, RBC count, platelet count 
D) RBC count, hematocrit, MCH

Question 18

Which analyzer DOES NOT use cyanide-free detection of hemoglobin?

Accepted Answer

A) Advia 120 
B) Advia 2120 
C) Abbott Cell-Dyn Sapphire 
D) Sysmex XE-2100 
A) Advia 120 
B) Advia 2120 
C) Abbott Cell-Dyn Sapphire 
D) Sysmex XE-2100

Question 19

Which parameter does the Sysmex XE-2100 instrument determine by light 	scattering and fluorescence intensity characteristics?

Accepted Answer

A)  Platelet count 
B)  Erythrocyte count 
C)  Absolute basophil count 
D)  Absolute lymphocyte count 
A)  Platelet count 
B)  Erythrocyte count 
C)  Absolute basophil count 
D)  Absolute lymphocyte count

Question 20

Which of the following instruments uses both electromechanical and optical density as a means of clot detection?

Accepted Answer

A) STArt 4 Coagulation system 
B) BBL Fibrosystem 
C) Trinity Biotech Coag-A-Mate XM 
D) AMAX Destiny Plus 
A) STArt 4 Coagulation system 
B) BBL Fibrosystem 
C) Trinity Biotech Coag-A-Mate XM 
D) AMAX Destiny Plus

Exam 35: Automation in Hematology and Hemostasis

What is the main difference between the methodologies employed on the Coulter Gen-S and the Coulter LH 780?

Which instrument does NOT use fluorescence intensity in its determination of reticulocytes?

Explain the purpose of the following used in the standard hematologic cell counting detection methods: A)VCS flow cell B)Photodetector C)Sheath fluid D)Direct current detection block

The principle that relies on detecting resistance as cells pass through a photo-detector and is that used by most of the major hematology analyzers is:

Which technologies are used to generate the LH 750's WBC scatterplot?

Which parameter does the Coulter LH 750 instrument derive from a histogram?

Using the chromogenic methodology, a coagulation protein's activity level is related to:

Using the Fibrometer instrument, clot detection occurs when:

What is the principle of impedance? Name at least two instruments that employ this method for blood cell counting.

In the reticulocyte determination, using the Sysmex XE-2100, the fluorescent dye containing oxazine and polymethine penetrates the cell membranes and stains the blood cells.What does the oxazine bind to within the cells?

The immunologic technique available on the AMAX Destiny Plus utilizes specific reagents with:

What is the purpose of the photodetector on the Diagnostica Stago STA Compact?

Regarding the CELL-DYN Sapphire, which parameter is determined by the impedance principle?

Which of the following represents the degree of light scatter that reflects cell complexity on the CELL-DYN Sapphire?

Compare and contrast the methods of detection for the following analyzers: A)Sysmex XE-2100 B)Abbott Cell-Dyn Sapphire C)Coulter LH 780 D)Advia 2120

Which analyzer measures platelets in the same channel as reticulocytes?

Which of the following parameters are calculated on the Coulter Gen-S?

Which analyzer DOES NOT use cyanide-free detection of hemoglobin?

Which parameter does the Sysmex XE-2100 instrument determine by light scattering and fluorescence intensity characteristics?

Which of the following instruments uses both electromechanical and optical density as a means of clot detection?

Introduction

Cellular Homeostasis

Hematopoiesis

Structure and Function of Hematopoietic Organs

The Erythrocyte

Hemoglobin

The Leukocyte

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopaties: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Introduction to Hemolytic Anemia

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Granulocyte and Monocyte Disorders

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Disorders

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Acute Lymphoblastic Leukemias

Lymphoid Malignancies: Chronic Lymphoid Leukemias, Lymphomas, and Plasma Cell Neoplasms

Hematopoietic Stem Cell Transplantation

Morphologic Analysis of Body Fluids in the Hematology Laboratory

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hematology Procedures and Hemostasis

Bone Marrow Examination

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Molecular Analysis of Hematologic Diseases

Laboratory Testing in Coagulation

Quality Assessment in the Hematology Laboratory

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