Question 1

Why is hydrodynamic focusing used in automated cell-counting instruments?

Accepted Answer

A) To prevent coincidence passage through the detection aperture 
B) To increase the visibility of the cells passing through the aperture 
C) To allow for maximal light absorbance through the cells passing through the aperture 
D) To ensure adequate uptake of fluorescent staining by the cells 
A) To prevent coincidence passage through the detection aperture 
B) To increase the visibility of the cells passing through the aperture 
C) To allow for maximal light absorbance through the cells passing through the aperture 
D) To ensure adequate uptake of fluorescent staining by the cells

Question 2

Which of the following analyzers uses radio frequency as a means of detecting and differentiating leukocytes?

Accepted Answer

A) Bayer Advia 2120 
B) Abbott Sapphire 
C) Sysmex XE-2100 
D) Coulter Gen-S 
A) Bayer Advia 2120 
B) Abbott Sapphire 
C) Sysmex XE-2100 
D) Coulter Gen-S

Question 3

Which analyzer has the ability to quantify nucleated RBCs and calculate a corrected WBC count?

Accepted Answer

A) Coulter Gen-S 
B) Sysmex XE-2100 
C) Abbott Cell Dyn Sapphire 
D) Coulter LH 780 
A) Coulter Gen-S 
B) Sysmex XE-2100 
C) Abbott Cell Dyn Sapphire 
D) Coulter LH 780

Question 4

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?

Accepted Answer

A) New methylene blue 
B) Propidium iodide 
C) Polymethine 
D) Oxazine 
A) New methylene blue 
B) Propidium iodide 
C) Polymethine 
D) Oxazine

Question 5

The Sysmex XE-2100 instrument printout reports a middle fluorescent ratio of 7.0 and a high fluorescent ratio of 1.3.What is the immature reticulocyte fraction?

Accepted Answer

A) 9.1 
B) 91.7 
C) 8.3 
D) 5.7 
A) 9.1 
B) 91.7 
C) 8.3 
D) 5.7

Question 6

When performing CBCs on the CELL-DYN Sapphire, a clinical laboratory professional may choose to run the ImmunoPltᵀᴹ assay if:

Accepted Answer

A)  Leukocyte count is decreased. 
B)  Erythrocyte fragments are detected. 
C)  Nucleated erythrocytes are observed. 
D)  Erythrocyte count is increased. 
A)  Leukocyte count is decreased. 
B)  Erythrocyte fragments are detected. 
C)  Nucleated erythrocytes are observed. 
D)  Erythrocyte count is increased.

Question 7

Choose the correct statement regarding chromogenic analysis in hemostasis testing from the choices listed below.

Accepted Answer

A) Chromogenic analysis is dependent upon the selected analyte's ability to cleave a chromophore. 
B) Chromogenic analysis is not subject to preanalytical problems, due to its mechanism of clot detection. 
C) There is no difference between chromogenic analysis and electromechanical detection. 
D) Chromogenic analysis is dependent upon the optical detection of ball movement through a steel tube as a clot forms. 
A) Chromogenic analysis is dependent upon the selected analyte's ability to cleave a chromophore. 
B) Chromogenic analysis is not subject to preanalytical problems, due to its mechanism of clot detection. 
C) There is no difference between chromogenic analysis and electromechanical detection. 
D) Chromogenic analysis is dependent upon the optical detection of ball movement through a steel tube as a clot forms.

Question 8

The immature platelet fraction (IPF) is a parameter derived from the Sysmex XE-2100's RET scattergram.An increased IPF is associated with:

Accepted Answer

A)  Aplastic anemia. 
B)  Pernicious anemia. 
C)  Acute lymphocytic leukemia. 
D)  Immune thrombocytopenic purpura. 
A)  Aplastic anemia. 
B)  Pernicious anemia. 
C)  Acute lymphocytic leukemia. 
D)  Immune thrombocytopenic purpura.

Question 9

Which instrument utilizes contour gating to classify different leukocyte cell types?

Accepted Answer

A)  ADVIA 120 
B)  XE-2100 
C)  LH 750 
D)  CELL-DYN Sapphire 
A)  ADVIA 120 
B)  XE-2100 
C)  LH 750 
D)  CELL-DYN Sapphire

Question 10

Which of the following analyzers measures reticulocytes by sphering the blood cells prior to analysis?

Accepted Answer

A) Coulter LH series 
B) Coulter Gen-S 
C) Abbott Sapphire 
D) Sysmex XE-2100 
A) Coulter LH series 
B) Coulter Gen-S 
C) Abbott Sapphire 
D) Sysmex XE-2100

Question 11

Cell volume and cell number are required elements for which of the following?

Accepted Answer

A) WBC scatterplots 
B) WBC cytograms 
C) RBC histograms 
D) RBC scattergrams 
A) WBC scatterplots 
B) WBC cytograms 
C) RBC histograms 
D) RBC scattergrams

Question 12

What cellular characteristic is evaluated by radio frequency?

Accepted Answer

A)  Cell size 
B)  Cell density 
C)  Cell shape 
D)  Cell complexity 
A)  Cell size 
B)  Cell density 
C)  Cell shape 
D)  Cell complexity

Question 13

Which of the following is measured immunologically on the Diagnostica Stago STA Compact?

Accepted Answer

A) von Willebrand factor 
B) D-dimer 
C) Lupus anticoagulants 
D) PT and APTT 
A) von Willebrand factor 
B) D-dimer 
C) Lupus anticoagulants 
D) PT and APTT

Question 14

Which of the following instruments relies on impedance to enumerate cells?

Accepted Answer

A) Gen-S 
B) XE-2100 
C) Sapphire 
D) All of the above 
A) Gen-S 
B) XE-2100 
C) Sapphire 
D) All of the above

Question 15

An analyzer is measuring a clot forming by the interaction of a stationary probe and a moving probe in a continuous motion.Suddenly the moving probe stops, and a clot is seen.Name the analyzer.

Accepted Answer

A) Diagnostica Stago 
B) MDA 180 
C) Trinity Biotech AMAX Destiny Plus 
D) BBL Fibrometer 
A) Diagnostica Stago 
B) MDA 180 
C) Trinity Biotech AMAX Destiny Plus 
D) BBL Fibrometer

Question 16

Name two analytes that can be measured immunologically, and list two instruments that currently use this method.

Accepted Answer

Two analytes: D-dimer and VWFT

Question 17

D-dimers are measured by which method on the Trinity Biotech AMAX Destiny Plus?

Accepted Answer

A) Electromechanical 
B) Optical density 
C) Chromogenic 
D) Immunologic 
A) Electromechanical 
B) Optical density 
C) Chromogenic 
D) Immunologic

Question 18

Which of the following is a reported parameter for the ADVIA 120 but not for the 	LH 750 instrument?

Accepted Answer

A)  RDW-CV 
B)  Retic% 
C)  CHCM 
D)  MCVr 
A)  RDW-CV 
B)  Retic% 
C)  CHCM 
D)  MCVr

Question 19

Which of the following instruments is capable of performing electromechanical, optical density, chromogenic, and immunologic methodologies?

Accepted Answer

A)  STArt 4 
B)  Destiny Plus 
C)  STA Compact 
D)  Coag-A-Mate XM 
A)  STArt 4 
B)  Destiny Plus 
C)  STA Compact 
D)  Coag-A-Mate XM

Exam 35: Automation in Hematology and Hemostasis

Why is hydrodynamic focusing used in automated cell-counting instruments?

Which of the following analyzers uses radio frequency as a means of detecting and differentiating leukocytes?

Which analyzer has the ability to quantify nucleated RBCs and calculate a corrected WBC count?

Which of the following binds to residual RNA in reticulocytes on the Sysmex XE-2100 analyzer?

The Sysmex XE-2100 instrument printout reports a middle fluorescent ratio of 7.0 and a high fluorescent ratio of 1.3.What is the immature reticulocyte fraction?

When performing CBCs on the CELL-DYN Sapphire, a clinical laboratory professional may choose to run the ImmunoPltᵀᴹ assay if:

Choose the correct statement regarding chromogenic analysis in hemostasis testing from the choices listed below.

The immature platelet fraction (IPF) is a parameter derived from the Sysmex XE-2100's RET scattergram.An increased IPF is associated with:

Which instrument utilizes contour gating to classify different leukocyte cell types?

Which of the following analyzers measures reticulocytes by sphering the blood cells prior to analysis?

Cell volume and cell number are required elements for which of the following?

What cellular characteristic is evaluated by radio frequency?

Which of the following is measured immunologically on the Diagnostica Stago STA Compact?

Which of the following instruments relies on impedance to enumerate cells?

An analyzer is measuring a clot forming by the interaction of a stationary probe and a moving probe in a continuous motion.Suddenly the moving probe stops, and a clot is seen.Name the analyzer.

Name two analytes that can be measured immunologically, and list two instruments that currently use this method.

D-dimers are measured by which method on the Trinity Biotech AMAX Destiny Plus?

Which of the following is a reported parameter for the ADVIA 120 but not for the LH 750 instrument?

Which of the following instruments is capable of performing electromechanical, optical density, chromogenic, and immunologic methodologies?

Introduction

Cellular Homeostasis

Hematopoiesis

Structure and Function of Hematopoietic Organs

The Erythrocyte

Hemoglobin

The Leukocyte

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopaties: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Introduction to Hemolytic Anemia

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Granulocyte and Monocyte Disorders

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Disorders

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Acute Lymphoblastic Leukemias

Lymphoid Malignancies: Chronic Lymphoid Leukemias, Lymphomas, and Plasma Cell Neoplasms

Hematopoietic Stem Cell Transplantation

Morphologic Analysis of Body Fluids in the Hematology Laboratory

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hematology Procedures and Hemostasis

Bone Marrow Examination

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Molecular Analysis of Hematologic Diseases

Laboratory Testing in Coagulation

Quality Assessment in the Hematology Laboratory

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