Question 1

Which component of the BM stroma is responsible for the formation of the ECM?

Accepted Answer

A) Cytokines 
B) Fibroblasts 
C) Osteoclasts 
D) Osteoblasts 
A) Cytokines 
B) Fibroblasts 
C) Osteoclasts 
D) Osteoblasts B

Question 2

Which of the following is a stem cell inhibitor?

Accepted Answer

A) Interferon 
B) MIP-1α 
C) Lactoferrin 
D) TNF-a 
A) Interferon 
B) MIP-1α 
C) Lactoferrin 
D) TNF-a B

Question 3

Explain the JAK-STAT signaling pathway, and describe how it can contribute to disease.

Accepted Answer

JAK: Janus family PTKs protein tyrosine kinases) interact with the STAT transcription factors signal transducers and activators of transcription).Once STAT proteins are phosphorylated by activated JAK kinases, they dimerize, translocate to the nucleus, bind to cytokine-specific DNA sequences, and activate or inhibit) specific gene expression.Abnormalities in this pathway have been shown to cause polycythemia vera and other myeloproliferative disorders..

Question 4

Explain the role of selected cytokines in the treatment of disorders affecting the hematopoietic system.

Accepted Answer

EPO: stimulation of erythropoi

Question 5

Signaling transduction pathways are propagated primarily through which of the following?

Accepted Answer

A) Kinases and protein phosphorylation 
B) Phosphatases and protein dephosphorylation 
C) Protein methylation 
D) Histone deacetylation 
A) Kinases and protein phosphorylation 
B) Phosphatases and protein dephosphorylation 
C) Protein methylation 
D) Histone deacetylation

Question 6

Which component allows the hematopoietic precursors to attach to the bone marrow microenvironment?

Accepted Answer

A) SCF 
B) FL 
C) CAM 
D) IFN 
A) SCF 
B) FL 
C) CAM 
D) IFN

Question 7

Which of the following is an example of a lineage-specific cytokine?

Accepted Answer

A) Kit ligand 
B) CFU-GEMM 
C) IL-3 
D) G-CSF 
A) Kit ligand 
B) CFU-GEMM 
C) IL-3 
D) G-CSF

Question 8

Explain how stromal cells contribute to the formation of the extracellular matrix.

Accepted Answer

The extracellular matrix is the hematopo

Question 9

An unknown cell is positive for CD34, and negative for CD38, lineage markers, and HLA-DR.Identify the cell.

Accepted Answer

A) MPP 
B) Hematopoietic stem cell 
C) CLP 
D) CMP 
A) MPP 
B) Hematopoietic stem cell 
C) CLP 
D) CMP

Question 10

All of the following are functions of the BM stroma except:

Accepted Answer

A) Adhesion of hematopoietic precursors. 
B) Cell-to-cell interaction. 
C) Synthesis of cytokines. 
D) Incorporation of essential metals into mature cells. 
A) Adhesion of hematopoietic precursors. 
B) Cell-to-cell interaction. 
C) Synthesis of cytokines. 
D) Incorporation of essential metals into mature cells.

Question 11

All of the following are key components of hematopoietic differentiation except:

Accepted Answer

A) The formation of mature blood components with a finite life span. 
B) The differentiation of blood components when exposed to different cytokines. 
C) The commitment to produce blood cells only. 
D) The activation of programmed cell death. 
A) The formation of mature blood components with a finite life span. 
B) The differentiation of blood components when exposed to different cytokines. 
C) The commitment to produce blood cells only. 
D) The activation of programmed cell death.

Question 12

Which of the following classes of hematopoietic cells is committed to becoming an erythrocyte?

Accepted Answer

A) Hematopoietic stem cell 
B) Multipotential progenitor cell 
C) Committed lymphoid progenitor 
D) BFU-E 
A) Hematopoietic stem cell 
B) Multipotential progenitor cell 
C) Committed lymphoid progenitor 
D) BFU-E

Question 13

Which of the following is given in synthetic form to patients with renal disease to stimulate red cell production?

Accepted Answer

A) GM-CSF 
B) Interleukin-2 
C) EPO 
D) G-CSF 
A) GM-CSF 
B) Interleukin-2 
C) EPO 
D) G-CSF

Question 14

The role of transcription factors (TF) in hematopoiesis is best described by which of the following statements?

Accepted Answer

A) TF expression does not change during hematopoiesis. 
B) TF function to either activate or suppress target genes. 
C) TF "instruct" the differentiation pathway for hematopoietic precursors. 
D) TF abnormalities are rarely seen in hematologic malignancies. 
A) TF expression does not change during hematopoiesis. 
B) TF function to either activate or suppress target genes. 
C) TF "instruct" the differentiation pathway for hematopoietic precursors. 
D) TF abnormalities are rarely seen in hematologic malignancies.

Question 15

All of the following can be formed from CFU-GEMM except:

Accepted Answer

A) Neutrophil. 
B) Erythrocyte. 
C) Platelet. 
D) Lymphocyte. 
A) Neutrophil. 
B) Erythrocyte. 
C) Platelet. 
D) Lymphocyte.

Question 16

Which of the following best describes the phenotype of the hematopoietic stem cell?

Accepted Answer

A) CD34+ CD38+Lin-HLA-DR-Rh123Bright, SCF-R+,TPO-R+ 
B) CD34+ CD33+CD38+Lin- Rh123Dull 
C) CD34+ CD38-Lin-HLA-DR-Rh123Dull, SCF-R+,TPO-R+ 
D) CD3+,CD4+,CD5+,CD8+,CD38-,HLA-DR- 
A) CD34+ CD38+Lin-HLA-DR-Rh123Bright, SCF-R+,TPO-R+ 
B) CD34+ CD33+CD38+Lin- Rh123Dull 
C) CD34+ CD38-Lin-HLA-DR-Rh123Dull, SCF-R+,TPO-R+ 
D) CD3+,CD4+,CD5+,CD8+,CD38-,HLA-DR-

Question 17

Regulation of stem cell homeostasis via SCF and Flt3 is predominantly via:

Accepted Answer

A) Autocrine signaling. 
B) Paracrine signaling. 
C) Juxtacrine signaling. 
D) Endocrine signaling. 
A) Autocrine signaling. 
B) Paracrine signaling. 
C) Juxtacrine signaling. 
D) Endocrine signaling.

Question 18

All of the following function primarily as multilineage (early acting) growth factors except:

Accepted Answer

A) IL-4. 
B) GM-CSF. 
C) IL-3. 
D) SCF. 
A) IL-4. 
B) GM-CSF. 
C) IL-3. 
D) SCF.

Question 19

Which components are included in the stromal cell compartment?

Accepted Answer

A) Matrix proteins and regulatory cytokines 
B) Adipocytes, endothelial cells, fibroblasts, T lymphocytes, and macrophages 
C) Stem cells 
D) Progenitor cells 
A) Matrix proteins and regulatory cytokines 
B) Adipocytes, endothelial cells, fibroblasts, T lymphocytes, and macrophages 
C) Stem cells 
D) Progenitor cells

Question 20

Which of the following classes of hematopoietic cells has the ability to self-renew?

Accepted Answer

A) Hematopoietic stem cell 
B) Multipotential progenitor cell 
C) Committed myeloid progenitor 
D) Maturing cells 
A) Hematopoietic stem cell 
B) Multipotential progenitor cell 
C) Committed myeloid progenitor 
D) Maturing cells

Exam 3: Hematopoiesis

Which component of the BM stroma is responsible for the formation of the ECM?

Which of the following is a stem cell inhibitor?

Explain the JAK-STAT signaling pathway, and describe how it can contribute to disease.

Explain the role of selected cytokines in the treatment of disorders affecting the hematopoietic system.

Signaling transduction pathways are propagated primarily through which of the following?

Which component allows the hematopoietic precursors to attach to the bone marrow microenvironment?

Which of the following is an example of a lineage-specific cytokine?

Explain how stromal cells contribute to the formation of the extracellular matrix.

An unknown cell is positive for CD34, and negative for CD38, lineage markers, and HLA-DR.Identify the cell.

All of the following are functions of the BM stroma except:

All of the following are key components of hematopoietic differentiation except:

Which of the following classes of hematopoietic cells is committed to becoming an erythrocyte?

Which of the following is given in synthetic form to patients with renal disease to stimulate red cell production?

The role of transcription factors (TF) in hematopoiesis is best described by which of the following statements?

All of the following can be formed from CFU-GEMM except:

Which of the following best describes the phenotype of the hematopoietic stem cell?

Regulation of stem cell homeostasis via SCF and Flt3 is predominantly via:

All of the following function primarily as multilineage (early acting) growth factors except:

Which components are included in the stromal cell compartment?

Which of the following classes of hematopoietic cells has the ability to self-renew?

Introduction

Cellular Homeostasis

Structure and Function of Hematopoietic Organs

The Erythrocyte

Hemoglobin

The Leukocyte

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopaties: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Introduction to Hemolytic Anemia

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Granulocyte and Monocyte Disorders

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Disorders

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Acute Lymphoblastic Leukemias

Lymphoid Malignancies: Chronic Lymphoid Leukemias, Lymphomas, and Plasma Cell Neoplasms

Hematopoietic Stem Cell Transplantation

Morphologic Analysis of Body Fluids in the Hematology Laboratory

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hematology Procedures and Hemostasis

Bone Marrow Examination

Automation in Hematology and Hemostasis

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Molecular Analysis of Hematologic Diseases

Laboratory Testing in Coagulation

Quality Assessment in the Hematology Laboratory

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