Question 1

A Hodgkin lymphoma patient is admitted for treatment.What would be the benefit in performing an EBV-DNA test on him?

Accepted Answer

A) It would identify the cause of the Hodgkin lymphoma. 
B) Since the patient is immunocompromised, it would identify an opportunistic pathogen. 
C) It would help monitor the efficacy of his treatment. 
D) It would help rule out other types of lymphomas. 
A) It would identify the cause of the Hodgkin lymphoma. 
B) Since the patient is immunocompromised, it would identify an opportunistic pathogen. 
C) It would help monitor the efficacy of his treatment. 
D) It would help rule out other types of lymphomas. C

Question 2

Choose the correct statement regarding molecular oncology from the choices below.

Accepted Answer

A) The genes responsible for tumor formation are oncogenes. 
B) Molecular testing is needed for cancer diagnosis. 
C) Molecular testing is not sensitive for monitoring minimal residual disease. 
D) All genetic mutations responsible for cancer can be identified by molecular techniques. 
A) The genes responsible for tumor formation are oncogenes. 
B) Molecular testing is needed for cancer diagnosis. 
C) Molecular testing is not sensitive for monitoring minimal residual disease. 
D) All genetic mutations responsible for cancer can be identified by molecular techniques. A

Question 3

Explain the central dogma of molecular biology using proper nomenclature.Define each phase.

Accepted Answer

The central dogma of molecular biology states that the functional unit of heredity DNA) is first replicated, then transcribed into messenger RNA and finally translated into a functional protein comprised of amino acids.
DNA is used to catalog, express, and propagate information.RNA is the messenger molecule that translates the DNA code so that proteins can be produced.

Question 4

Mutations responsible for the onset of lymphoid leukemias and lymphomas are typically associated with:

Accepted Answer

A) Antigen receptor genes. 
B) Receptors for oncogenes. 
C) Specific chromosomal translocations. 
D) CD marker alterations. 
A) Antigen receptor genes. 
B) Receptors for oncogenes. 
C) Specific chromosomal translocations. 
D) CD marker alterations.

Question 5

Explain the impact of a positive test for HTLV-1 in a lymphoma patient.

Accepted Answer

HTLV1 infects T-helper lymphocytes and c

Question 6

Name and compare two molecular methods that are used to detect mutations associated with sickle-cell disease.

Accepted Answer

Two molecular methods that can be used t

Question 7

Which of the following assays would be most appropriate for monitoring minimal residual disease?

Accepted Answer

A) Karyotyping 
B) Southern Blot analysis 
C) PCR 
D) All of the above 
A) Karyotyping 
B) Southern Blot analysis 
C) PCR 
D) All of the above

Question 8

A 23-year-old female is admitted to the local hospital with a deep vein thrombosis.Besides initial laboratory workup, which of the following should be included to investigate possible causes of the DVT?

Accepted Answer

A) PCR for sickle-cell mutation 
B) FV and prothrombin mutations 
C) BCR/ABL1 fusion gene mutation 
D) TCR gene mutations 
A) PCR for sickle-cell mutation 
B) FV and prothrombin mutations 
C) BCR/ABL1 fusion gene mutation 
D) TCR gene mutations

Question 9

Give the advanatgaes of PCR over Southern Blot and advantages of Southern Blot over PCR.

Accepted Answer

Advantages of PCR over Southern Blot: sm

Question 10

Which of the following methods would be most useful for identifying point mutations anywhere in the DNA segment?

Accepted Answer

A) Southern Blot analysis 
B) PCR 
C) DNA sequencing 
D) FISH 
A) Southern Blot analysis 
B) PCR 
C) DNA sequencing 
D) FISH

Question 11

Which of the following is associated with chronic myelogenous leukemia?

Accepted Answer

A) BCR/ABL1 fusion gene 
B) t(15;17) 
C) t(8,14) 
D) PML/RAR fusion gene 
A) BCR/ABL1 fusion gene 
B) t(15;17) 
C) t(8,14) 
D) PML/RAR fusion gene

Question 12

Inherited diseases are characterized by:

Accepted Answer

A) Sex-linked mutations that are expressed only in females. 
B) Acquired mutations that are detected only in affected organs. 
C) Lack of correlation between genotype and disease status among family members. 
D) A genetic defect that is generally present in all tissues of the patient's body. 
A) Sex-linked mutations that are expressed only in females. 
B) Acquired mutations that are detected only in affected organs. 
C) Lack of correlation between genotype and disease status among family members. 
D) A genetic defect that is generally present in all tissues of the patient's body.

Question 13

Explain why molecular testing is essential not only in identifying disorders, such as cancer, but also during the course of a disease.

Accepted Answer

Unlike inherited disease, in which every

Question 14

What is RNA?

Accepted Answer

A) A fundamental substance of heredity that cells use to catalog, express, and propagate information 
B) A messenger molecule that translates the DNA code to induce protein production 
C) The functional unit comprised of amino acids 
D) Proteins translated from DNA 
A) A fundamental substance of heredity that cells use to catalog, express, and propagate information 
B) A messenger molecule that translates the DNA code to induce protein production 
C) The functional unit comprised of amino acids 
D) Proteins translated from DNA

Question 15

In reference to detecting minimal residual disease, which of the following is true?

Accepted Answer

A) PCR, karyotyping and Southern blot analysis are equally sensitive and specific. 
B) PCR is most sensitive for detecting low levels of tumor burden. 
C) Karyotyping is capable of detecting 0.1% tumor burden. 
D) Southern Blot analysis has 100% sensitivity and specificity for detecting aberrant proteins formed as a result of the genetic mutation. 
A) PCR, karyotyping and Southern blot analysis are equally sensitive and specific. 
B) PCR is most sensitive for detecting low levels of tumor burden. 
C) Karyotyping is capable of detecting 0.1% tumor burden. 
D) Southern Blot analysis has 100% sensitivity and specificity for detecting aberrant proteins formed as a result of the genetic mutation.

Exam 38: Molecular Analysis of Hematologic Diseases

A Hodgkin lymphoma patient is admitted for treatment.What would be the benefit in performing an EBV-DNA test on him?

Choose the correct statement regarding molecular oncology from the choices below.

Explain the central dogma of molecular biology using proper nomenclature.Define each phase.

Mutations responsible for the onset of lymphoid leukemias and lymphomas are typically associated with:

Explain the impact of a positive test for HTLV-1 in a lymphoma patient.

Name and compare two molecular methods that are used to detect mutations associated with sickle-cell disease.

Which of the following assays would be most appropriate for monitoring minimal residual disease?

A 23-year-old female is admitted to the local hospital with a deep vein thrombosis.Besides initial laboratory workup, which of the following should be included to investigate possible causes of the DVT?

Give the advanatgaes of PCR over Southern Blot and advantages of Southern Blot over PCR.

Which of the following methods would be most useful for identifying point mutations anywhere in the DNA segment?

Which of the following is associated with chronic myelogenous leukemia?

Inherited diseases are characterized by:

Explain why molecular testing is essential not only in identifying disorders, such as cancer, but also during the course of a disease.

What is RNA?

In reference to detecting minimal residual disease, which of the following is true?

Introduction

Cellular Homeostasis

Hematopoiesis

Structure and Function of Hematopoietic Organs

The Erythrocyte

Hemoglobin

The Leukocyte

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopaties: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Introduction to Hemolytic Anemia

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Granulocyte and Monocyte Disorders

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Disorders

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Acute Lymphoblastic Leukemias

Lymphoid Malignancies: Chronic Lymphoid Leukemias, Lymphomas, and Plasma Cell Neoplasms

Hematopoietic Stem Cell Transplantation

Morphologic Analysis of Body Fluids in the Hematology Laboratory

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hematology Procedures and Hemostasis

Bone Marrow Examination

Automation in Hematology and Hemostasis

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Laboratory Testing in Coagulation

Quality Assessment in the Hematology Laboratory

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