Exam 27: Morphologic Analysis of Body Fluids in the Hematology Laboratory

All of the following can be found in normal body fluid except:

What is the significance of micro-organisms present in the cytospin?

An overweight 73-year-old man sees his orthopedic specialist because of recurrent right knee pain.The physician performs an arthrocentesis and sends it to the laboratory for analysis.The technologist notes many crystals with notched edges exhibiting strong birefringence.Based on this information, from what is the patient most likely suffering?

An unknown cell in a body fluid possesses the following morphologic characteristics: 35 μm in size, partially clumped chromatin, prominent nucleoli, jagged nuclear membrane, and clumped cell with nuclear molding.What is the most likely identification of the unknown cell?

Interpret the following results: -Specimen: cerebrospinal fluid (CSF) -Tubes sent: 1-4 -Tube 1 RBC count: 30,000/ul -Tube 2 RBC count: 1500/ul -Tube 3 RBC count: 200/ul -Tube 4 RBC count: 4/ul -Gross examination: -Tube 1: moderate hemolysis, clear -Tube 2: slight hemolysis, clear -Tube 3: no hemolysis, clear -Tube 4: no hemolysis, clear -Xanthochromia: absent in all four tubes -Microscopic findings: -Many intact RBCs -0-5 neutrophils -0-5 lymphocytes -0-5 MNCs What do the data above most likely represent?

Explain how the following are formed: a.Transudate b.Exudate c.Chylous fluid

Artifacts that can be seen with cytocentrifuge-prepared slides can be interpreted as a clinically significant finding by mistake.Which of the following can be an artifact leading to a mistaken interpretation of bacterial organisms in a fluid?

Interpret the following results: Specimen: pleural effusion on a 45-year-old lung cancer patient: -Specific gravity = 1.025 -Total protein = 4.5 g/dl -F:Se protein = 0.9 -Fluid LD/serum LD = 0.8 -Total WBC count = 2,205/ul What is the classification of this fluid?

This technique should be done to differentiate starch particles from pathogenic crystals in fluids:

A 3-year-old boy is admitted with febrile seizures.A lumbar puncture is performed to rule out meningitis.The cytospin differential shows many large lymphoblasts with irregular nuclear membranes.What does this mean for the patient?

List common morphologic changes associated with cytocentrifugation.

One of the definitive signs of hemorrhage in the CNS is:

An effusion can accumulate due to a systemic disease, as with congestive heart failure.The correct term for this fluid is:

A crystal with long, thin, needlelike, pointed ends is identified in synovial fluid.The crystals are located within the cells and outside the cells.A quartz compensator is used, and it is determined that the crystals are negatively birefringent, and are a yellow color when parallel to the axis of the compensator.The crystal is blue when turned perpendicular to the axis of the compensator.What is the type of crystal?

What type of fluid is collected from the spinal tap or lumbar puncture procedure?

Morphologic evaluation of body fluids prepared by using the cytocentrifuge is an excellent method of concentration of cells for microscopic review.When the pleural fluid is cytocentrifuged for staining, what type of normal cells can be expected to be seen?

Which procedure is used to collect the pleural fluid from the pleural cavity?

A peritoneal dialysate fluid is sent to the laboratory for analysis.Patient history reveals an 85-year-old woman with a previous history of duodenal cancer that is in remission.The technologist is reviewing the cytospin, and notices many large cells that are smooth in appearance and have abundant, basophilic cytoplasm with smooth nuclear membrane, evenly distributed chromatin, and no nucleoli.What is the most probable identification of those cells?

Accumulation of fluid as a result of systemic disease is defined as:

Malignant tissue cells have which of the following morphologic features?