Question 1

An unknown cell resembling a hematogone has CD19 and CD20 antigens.What is the lineage of the unknown cell?

Accepted Answer

A) Myeloid 
B) Monocytic 
C) Lymphoid 
D) Erythroid 
A) Myeloid 
B) Monocytic 
C) Lymphoid 
D) Erythroid C

Question 2

In diagnosing PNH, which of the following assays is most sensitive to the detection of the disorder?

Accepted Answer

A) GPI anchor phenotype 
B) Ham test 
C) Sucrose lysis test 
D) Osmotic fragility test 
A) GPI anchor phenotype 
B) Ham test 
C) Sucrose lysis test 
D) Osmotic fragility test A

Question 3

CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation.The EWGCCA recommendations for this enumeration include proper technique and interpretation of the flow cytometry analysis.Which of the following standards were recommended for proper acquisition of events?

Accepted Answer

A) Include platelets and unlysed red blood cells and debris 
B) Include only CD34 dim staining populations 
C) Use bright fluorochrome conjugates of class II or III monoclonal antibodies that detect all glycoforms of CD34 
D) Acquire only 10 CD34 positive cells 
A) Include platelets and unlysed red blood cells and debris 
B) Include only CD34 dim staining populations 
C) Use bright fluorochrome conjugates of class II or III monoclonal antibodies that detect all glycoforms of CD34 
D) Acquire only 10 CD34 positive cells C

Question 4

Which of the following is considered a drawback of immunophenotyping a suspected acute leukemia?

Accepted Answer

A) AML NOC 
B) Lineage heterogeneity 
C) Lack of sensitivity 
D) All of the above 
A) AML NOC 
B) Lineage heterogeneity 
C) Lack of sensitivity 
D) All of the above

Question 5

Neoplasms are made up of a population of identical cells, or a clone of cells.A clone, when identified, can lead to discovery of a lymphoid malignancy or other lymphoproliferative disorder.In what ways can clonality be detected?

Accepted Answer

A) Uniform expression of one immunoglobulin light chain 
B) Mixture of cells with either kappa or lambda light chains 
C) Surface immunoglobulin detection 
D) Bright intensity of all monoclonal markers 
A) Uniform expression of one immunoglobulin light chain 
B) Mixture of cells with either kappa or lambda light chains 
C) Surface immunoglobulin detection 
D) Bright intensity of all monoclonal markers

Question 6

Choose the correct statement regarding the IRF from the choices below:

Accepted Answer

A) The IRF is directly proportional to the degree of bone marrow turnover. 
B) The IRF is indirectly proportional to the average age of the reticulocytes. 
C) The IRF cannot be used to monitor erythropoietin therapy. 
D) The IRF cannot be used to monitor bone marrow recovery after transplant. 
A) The IRF is directly proportional to the degree of bone marrow turnover. 
B) The IRF is indirectly proportional to the average age of the reticulocytes. 
C) The IRF cannot be used to monitor erythropoietin therapy. 
D) The IRF cannot be used to monitor bone marrow recovery after transplant.

Question 7

Which analyte is the best indicator of the overall picture of the immune system in an HIV-positive patient?

Accepted Answer

A) Absolute CD4 count 
B) Relative lymphocyte count 
C) WBC count 
D) All of the above 
A) Absolute CD4 count 
B) Relative lymphocyte count 
C) WBC count 
D) All of the above

Question 8

The fluorescent dye employed by hematology analyzers to count reticulocytes binds to:

Accepted Answer

A) DNA. 
B) Proteins. 
C) RNA. 
D) Nucleic acids. 
A) DNA. 
B) Proteins. 
C) RNA. 
D) Nucleic acids.

Question 9

What is one advantage to using monoclonal antibodies, rather than polyclonal antibodies, in immunophenotyping?

Accepted Answer

A) They are commercially available. 
B) The antigen can be recognized even if some parts of it are abnormal. 
C) They have high purity and reproducibility. 
D) They can be directly attached to fluorochromes. 
A) They are commercially available. 
B) The antigen can be recognized even if some parts of it are abnormal. 
C) They have high purity and reproducibility. 
D) They can be directly attached to fluorochromes.

Question 10

Explain the advantages of using flow cytometry to count reticulocytes.

Accepted Answer

Quantification of reticulocytes using ma

Question 11

Which two CD markers would be helpful in differentially identifying CLL from hairy cell leukemia?

Accepted Answer

A) CD19 and CD5 
B) CD5 and CD11c 
C) CD5 and CD25 
D) sIg and CD25 
A) CD19 and CD5 
B) CD5 and CD11c 
C) CD5 and CD25 
D) sIg and CD25

Question 12

What is the purpose of CD34 count?

Accepted Answer

To quantify stem cel

Question 13

Name at least two pitfalls in immunophenotyping mature lymphoid malignancies, and explain how you would correct this.

Accepted Answer

Some malignant B- cell lymphoproliferati

Question 14

How can flow cytometry be used in cell quantitation?

Accepted Answer

Flow cytometry can be used in

Question 15

Cells can be separated during data analysis by gating.This process of isolating cells can be based on what properties?

Accepted Answer

A) Cell size and granularity 
B) Cell density 
C) Shape of the nucleus 
D) Color of the cytoplasm 
A) Cell size and granularity 
B) Cell density 
C) Shape of the nucleus 
D) Color of the cytoplasm

Question 16

It is recommended by the EWGCCA to perform which of the following alongside CD34 enumeration?

Accepted Answer

A) Dual staining with CD45 
B) DNA index 
C) IRF 
D) All of the above 
A) Dual staining with CD45 
B) DNA index 
C) IRF 
D) All of the above

Question 17

Which of the following is/are appropriate specimen(s) for flow cytometry?

Accepted Answer

A) Blood 
B) Bone marrow 
C) Lymphoid tissue 
D) All of the above 
A) Blood 
B) Bone marrow 
C) Lymphoid tissue 
D) All of the above

Question 18

The flow cytometer contains a flow chamber.This chamber has two columns of fluid maintained at different pressure, sample and sheath fluid.Which of the following statements about the flow chamber is correct?

Accepted Answer

A) Cells in the outer column become dispersed. 
B) The sample fluid has turbulent flow. 
C) Cells are aligned in single file in front of the laser. 
D) The two columns of fluid differ in color. 
A) Cells in the outer column become dispersed. 
B) The sample fluid has turbulent flow. 
C) Cells are aligned in single file in front of the laser. 
D) The two columns of fluid differ in color.

Question 19

DNA content of a patient's tumor cells is reported as 2.0, and the DNA content of diploid cells is 1.0.What is the DNA index?

Accepted Answer

A) 2.0 
B) 3.0 
C) 1.0 
D) 0.5 
A) 2.0 
B) 3.0 
C) 1.0 
D) 0.5

Question 20

A single group of cells possessing identical aberrant phenotypes is defined as:

Accepted Answer

A) Monoclonal. 
B) Clonality. 
C) Tumorigenic. 
D) Contour gating. 
A) Monoclonal. 
B) Clonality. 
C) Tumorigenic. 
D) Contour gating.

Exam 36: Flow Cytometry

An unknown cell resembling a hematogone has CD19 and CD20 antigens.What is the lineage of the unknown cell?

In diagnosing PNH, which of the following assays is most sensitive to the detection of the disorder?

CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation.The EWGCCA recommendations for this enumeration include proper technique and interpretation of the flow cytometry analysis.Which of the following standards were recommended for proper acquisition of events?

Which of the following is considered a drawback of immunophenotyping a suspected acute leukemia?

Neoplasms are made up of a population of identical cells, or a clone of cells.A clone, when identified, can lead to discovery of a lymphoid malignancy or other lymphoproliferative disorder.In what ways can clonality be detected?

Choose the correct statement regarding the IRF from the choices below:

Which analyte is the best indicator of the overall picture of the immune system in an HIV-positive patient?

The fluorescent dye employed by hematology analyzers to count reticulocytes binds to:

What is one advantage to using monoclonal antibodies, rather than polyclonal antibodies, in immunophenotyping?

Explain the advantages of using flow cytometry to count reticulocytes.

Which two CD markers would be helpful in differentially identifying CLL from hairy cell leukemia?

What is the purpose of CD34 count?

Name at least two pitfalls in immunophenotyping mature lymphoid malignancies, and explain how you would correct this.

How can flow cytometry be used in cell quantitation?

Cells can be separated during data analysis by gating.This process of isolating cells can be based on what properties?

It is recommended by the EWGCCA to perform which of the following alongside CD34 enumeration?

Which of the following is/are appropriate specimen(s) for flow cytometry?

The flow cytometer contains a flow chamber.This chamber has two columns of fluid maintained at different pressure, sample and sheath fluid.Which of the following statements about the flow chamber is correct?

DNA content of a patient's tumor cells is reported as 2.0, and the DNA content of diploid cells is 1.0.What is the DNA index?

A single group of cells possessing identical aberrant phenotypes is defined as:

Introduction

Cellular Homeostasis

Hematopoiesis

Structure and Function of Hematopoietic Organs

The Erythrocyte

Hemoglobin

The Leukocyte

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopaties: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Introduction to Hemolytic Anemia

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Granulocyte and Monocyte Disorders

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Disorders

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Acute Lymphoblastic Leukemias

Lymphoid Malignancies: Chronic Lymphoid Leukemias, Lymphomas, and Plasma Cell Neoplasms

Hematopoietic Stem Cell Transplantation

Morphologic Analysis of Body Fluids in the Hematology Laboratory

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hematology Procedures and Hemostasis

Bone Marrow Examination

Automation in Hematology and Hemostasis

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Molecular Analysis of Hematologic Diseases

Laboratory Testing in Coagulation

Quality Assessment in the Hematology Laboratory

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