Question 1

One of the disadvantages of using autologous stem cells for treatment in a leukemia patient is:

Accepted Answer

A) The graft-versus-leukemia effect is not possible. 
B) There is increased risk for graft-versus-host disease. 
C) There is not a need for immunosuppression. 
D) The yield of stem cells is low. 
A) The graft-versus-leukemia effect is not possible. 
B) There is increased risk for graft-versus-host disease. 
C) There is not a need for immunosuppression. 
D) The yield of stem cells is low.

Question 2

Following a stem cell transplant, what steps are identified in the evaluation of engraftment of stem cells?

Accepted Answer

A) Chimerism studies, bone marrow M:E ratio and cellularity 
B) CD34 enumeration of peripheral blood and bone marrow cellularity 
C) Absolute lymphocyte count, chimerism studies and bone marrow cellularity 
D) Absolute neutrophil count enumeration, and platelet count and chimerism studies 
A) Chimerism studies, bone marrow M:E ratio and cellularity 
B) CD34 enumeration of peripheral blood and bone marrow cellularity 
C) Absolute lymphocyte count, chimerism studies and bone marrow cellularity 
D) Absolute neutrophil count enumeration, and platelet count and chimerism studies

Question 3

Which of the following types of SCT patients have the highest risk of developing GVHD?

Accepted Answer

A) Syngeneic transplant patients 
B) Autologous transplant patients 
C) Allogeneic transplant patients Correct 
A) Syngeneic transplant patients 
B) Autologous transplant patients 
C) Allogeneic transplant patients Correct

Question 4

Which laboratory department is responsible for performing testing to verify short-term engraftment?

Accepted Answer

A) Hematology 
B) Molecular/HLA 
C) Microbiology 
D) Immunology 
A) Hematology 
B) Molecular/HLA 
C) Microbiology 
D) Immunology

Question 5

Which of the following laboratory tests is used to assess long-term engraftment?

Accepted Answer

A) Automated complete blood count 
B) Chimerism 
C) CD34 count by flow 
D) Manual WBC count 
A) Automated complete blood count 
B) Chimerism 
C) CD34 count by flow 
D) Manual WBC count

Question 6

When calculating the dose of stem cells needed for the recipient, what factors must be considered?

Accepted Answer

A) When using umbilical cord blood, a minimum of 3.0 x 105 of total nucleated cells per kilogram recipient weight 
B) Cell doses of a minimum of 2.5-5.0 x 106 CD34+ cells per kilogram recipient weight 
C) Cell doses of a minimum of 0.5-1.5 x 103 CD56+ cells per kilogram recipient weight 
D) When using umbilical cord blood, a minimum of 1.0 x 103 of total nucleated cells per kilogram recipient weight. 
A) When using umbilical cord blood, a minimum of 3.0 x 105 of total nucleated cells per kilogram recipient weight 
B) Cell doses of a minimum of 2.5-5.0 x 106 CD34+ cells per kilogram recipient weight 
C) Cell doses of a minimum of 0.5-1.5 x 103 CD56+ cells per kilogram recipient weight 
D) When using umbilical cord blood, a minimum of 1.0 x 103 of total nucleated cells per kilogram recipient weight.

Question 7

Which of the following best represents the correct order of maturation (youngest to most mature) for hematopoiesis?

Accepted Answer

A) HSC > precursor >committed progenitor 
B) Precursor >HSC >committed progenitor 
C) HSC > committed progenitor > precursor 
D) Committed progenitor >precursor > HSC 
A) HSC > precursor >committed progenitor 
B) Precursor >HSC >committed progenitor 
C) HSC > committed progenitor > precursor 
D) Committed progenitor >precursor > HSC

Question 8

The laboratory professional is involved in which of following steps associated with SCT?

Accepted Answer

A) Compatibility testing 
B) Pretransfusion testing 
C) Apheresis 
D) All of the above 
A) Compatibility testing 
B) Pretransfusion testing 
C) Apheresis 
D) All of the above

Question 9

Which of the following disorders can be treated by stem cell transplantation?

Accepted Answer

A) Leukemia 
B) Aplastic anemia 
C) Sickle-cell disease 
D) All of the above 
A) Leukemia 
B) Aplastic anemia 
C) Sickle-cell disease 
D) All of the above

Question 10

Following apheresis of a patient for an autologous stem cell transplant, the laboratory professional will:

Accepted Answer

A) Freeze the cells in bags with DMSO. 
B) Perform WBC, mononuclear cell,and CD34 cell count. 
C) Purge the sample of all T8 cells. 
D) Perform chimerism studies. 
A) Freeze the cells in bags with DMSO. 
B) Perform WBC, mononuclear cell,and CD34 cell count. 
C) Purge the sample of all T8 cells. 
D) Perform chimerism studies.

Question 11

Stem cells can treat a variety of different disorders due to their ability to:

Accepted Answer

A) Self-renew. 
B) Give rise to other cells. 
C) Regenerate quickly. 
D) Possess immunity against toxic agents. 
A) Self-renew. 
B) Give rise to other cells. 
C) Regenerate quickly. 
D) Possess immunity against toxic agents.

Question 12

Explain the significance of HLA and ABO antigen compatibility in stem cell transplantation.

Accepted Answer

Compatibility between donor and recipien

Question 13

Explain the role of the clinical laboratory professional in stem cell transplantation.

Accepted Answer

The role of the laboratory professional

Question 14

When the decision is made to undertake the stem cell transplantation process, many laboratories are involved in working for a successful outcome.The MNCs product will go to which laboratories for cell counts and immunotyping?

Accepted Answer

A) Molecular diagnostic and HLA labs 
B) Hematology and flow cytometry labs 
C) Blood bank and HLA labs 
D) Molecular diagnostics and hematology labs 
A) Molecular diagnostic and HLA labs 
B) Hematology and flow cytometry labs 
C) Blood bank and HLA labs 
D) Molecular diagnostics and hematology labs

Question 15

How are HSCs different from committed progenitor cells?

Accepted Answer

A) Their ability to self-renew 
B) Their success in autologous transplantation 
C) Their potency in the immune response 
D) Their location of maturation 
A) Their ability to self-renew 
B) Their success in autologous transplantation 
C) Their potency in the immune response 
D) Their location of maturation

Question 16

Engraftment after transplant is determined by short-term evidence of hematopoietic proliferation.What are the peripheral blood absolute cell counts that indicate short-term engraftment?

Accepted Answer

A) Absolute neutrophil count is more than 100 µL and platelet count is more than 50,000 µL. 
B) Absolute lymphocyte count is less than 500 µL and platelet count is more than 10,000 µL. 
C) Neutrophil count is more than 500 µL and the platelet count is more than 20,000 µL. 
D) Neutrophil count is more than 1000 µL and the platelet count is more than 10,000 µL. 
A) Absolute neutrophil count is more than 100 µL and platelet count is more than 50,000 µL. 
B) Absolute lymphocyte count is less than 500 µL and platelet count is more than 10,000 µL. 
C) Neutrophil count is more than 500 µL and the platelet count is more than 20,000 µL. 
D) Neutrophil count is more than 1000 µL and the platelet count is more than 10,000 µL.

Question 17

What is a primary disadvantage of transfusing umbilical cord blood as a source of HSCs?

Accepted Answer

A) The risk of viral infection is high. 
B) It needs to be frozen soon after collection. 
C) There are complications associated with the collection. 
D) The number of stem cells is usually insufficient for an adult transplant. 
A) The risk of viral infection is high. 
B) It needs to be frozen soon after collection. 
C) There are complications associated with the collection. 
D) The number of stem cells is usually insufficient for an adult transplant.

Question 18

Which of the following occurs prior to infusion of stem cells into the recipient?

Accepted Answer

A) Blood count for ANC 
B) Chimerism studies 
C) GM-CSF infusion 
D) CD34 enumeration 
A) Blood count for ANC 
B) Chimerism studies 
C) GM-CSF infusion 
D) CD34 enumeration

Question 19

Which HSC quantitation method would be optimal for verification of late-stage engraftment?

Accepted Answer

A) Manual count of MNCs 
B) CD34 enumeration by flow cytometry 
C) Cell culture for CFUs 
D) All of the above 
A) Manual count of MNCs 
B) CD34 enumeration by flow cytometry 
C) Cell culture for CFUs 
D) All of the above

Question 20

A major limitation of using cord blood for stem cell transplants is:

Accepted Answer

A) Number of stem cells is inadequate for most adults. 
B) Collecting cord blood is dangerous for the baby. 
C) The risk of bacterial contamination is great. 
D) It does not contain CD4 lymphocytes. 
A) Number of stem cells is inadequate for most adults. 
B) Collecting cord blood is dangerous for the baby. 
C) The risk of bacterial contamination is great. 
D) It does not contain CD4 lymphocytes.

Exam 26: Hematopoietic Stem Cell Transplantation

One of the disadvantages of using autologous stem cells for treatment in a leukemia patient is:

Following a stem cell transplant, what steps are identified in the evaluation of engraftment of stem cells?

Which of the following types of SCT patients have the highest risk of developing GVHD?

Which laboratory department is responsible for performing testing to verify short-term engraftment?

Which of the following laboratory tests is used to assess long-term engraftment?

When calculating the dose of stem cells needed for the recipient, what factors must be considered?

Which of the following best represents the correct order of maturation (youngest to most mature) for hematopoiesis?

The laboratory professional is involved in which of following steps associated with SCT?

Which of the following disorders can be treated by stem cell transplantation?

Following apheresis of a patient for an autologous stem cell transplant, the laboratory professional will:

Stem cells can treat a variety of different disorders due to their ability to:

Explain the significance of HLA and ABO antigen compatibility in stem cell transplantation.

Explain the role of the clinical laboratory professional in stem cell transplantation.

When the decision is made to undertake the stem cell transplantation process, many laboratories are involved in working for a successful outcome.The MNCs product will go to which laboratories for cell counts and immunotyping?

How are HSCs different from committed progenitor cells?

Engraftment after transplant is determined by short-term evidence of hematopoietic proliferation.What are the peripheral blood absolute cell counts that indicate short-term engraftment?

What is a primary disadvantage of transfusing umbilical cord blood as a source of HSCs?

Which of the following occurs prior to infusion of stem cells into the recipient?

Which HSC quantitation method would be optimal for verification of late-stage engraftment?

A major limitation of using cord blood for stem cell transplants is:

Introduction

Cellular Homeostasis

Hematopoiesis

Structure and Function of Hematopoietic Organs

The Erythrocyte

Hemoglobin

The Leukocyte

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopaties: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Introduction to Hemolytic Anemia

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Granulocyte and Monocyte Disorders

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Disorders

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Acute Lymphoblastic Leukemias

Lymphoid Malignancies: Chronic Lymphoid Leukemias, Lymphomas, and Plasma Cell Neoplasms

Morphologic Analysis of Body Fluids in the Hematology Laboratory

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hematology Procedures and Hemostasis

Bone Marrow Examination

Automation in Hematology and Hemostasis

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Molecular Analysis of Hematologic Diseases

Laboratory Testing in Coagulation

Quality Assessment in the Hematology Laboratory

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