Exam 18: Recombinent Dna and Biotechnology

Describe three useful products that have been produced using biotechnology. Outline two specific dangers that could result from producing organisms that contain foreign genes.

Use the following to answer questions: The diagram below shows the steps used in microarray analysis to detect differences in expression between tumor tissue and normal tissue. -At which step does hybridization with oligonucleotides occur?

Humans have been using biotechnology for _______ years.

Which of the following is not an application of recombinant DNA technology?

Proteins that are synthesized in the cytoplasm of eukaryotic cells may be targeted to many places within the cell. How might you use rDNA methods to discern where the information is for the targeting of a particular protein?

Variation at a specific gene is known to affect metabolism in mice, but it is not clear which of the nucleotides are responsible for the changes. In addition to recombinant DNA technology, which the following techniques would be most relevant for revealing which of the nucleotides affect metabolism?

The use of which of the following would be most appropriate for inhibiting transcription of a specific gene?

Which of the following DNA sequences would most likely be recognized by a restriction enzyme?

For a Ti plasmid to be propagated in a host plant cell, the vector needs

All other things being equal, which of the following genetically altered foods would most likely be of concern regarding human health?

For which of the following processes would we use homologous recombination?

A vector designed for the expression of a eukaryotic transgene in E. coli requires

Which of the following best describes the major advantage of using microarrays to detect gene expression differences across the genome, as compared with hybridization technologies?

The primary benefit of using genetic markers in plant breeding is that this technology

Which of the following statements about bacterial antibiotic resistance genes is false?

The process of inserting recombinant DNA is considered transfection when

For which of the following techniques is the synthesis of oligonucleotides most directly useful?

Use the following to answer questions: The diagram below shows the steps used in microarray analysis to detect differences in expression between tumor tissue and normal tissue. -At which step is antisense RNA used?

The use of living cells to produce materials that are useful to people is known as _______.

Use the following to answer questions : The diagram below shows how knockout mice are constructed. -When does homologous recombination take place?