Question 1

A plasmid library and a phage library are constructed from the same genetic material. Which of the following statements is true?

Accepted Answer

A)  The phage library has more clones than the plasmid library because each phage clone contains less DNA. 
B)  The phage library has fewer clones than the plasmid library because each phage clone contains more DNA. 
C)  The phage library has fewer clones than the plasmid library because phage libraries do not include nontranscribed DNA. 
D)  The plasmid library has fewer clones than the phage library because plasmid libraries do not include nontranscribed DNA. 
E)  The phage library has fewer clones than the plasmid library because phage libraries do not include nontranslated DNA. 
A)  The phage library has more clones than the plasmid library because each phage clone contains less DNA. 
B)  The phage library has fewer clones than the plasmid library because each phage clone contains more DNA. 
C)  The phage library has fewer clones than the plasmid library because phage libraries do not include nontranscribed DNA. 
D)  The plasmid library has fewer clones than the phage library because plasmid libraries do not include nontranscribed DNA. 
E)  The phage library has fewer clones than the plasmid library because phage libraries do not include nontranslated DNA.

Question 2

Methods developed by the _______ industry were most directly responsible for the development of DNA microarray technologies.

Accepted Answer

A)  telecommunications 
B)  synthetic polymer 
C)  aerospace 
D)  semiconductor 
E)  automobile 
A)  telecommunications 
B)  synthetic polymer 
C)  aerospace 
D)  semiconductor 
E)  automobile

Question 3

Laura van 't Veer and her colleagues discovered specific differences between cancer patients with good prognoses and those with poor prognoses. The technique most closely associated with the diagnostic tool they invented is

Accepted Answer

A)  DNA fingerprinting. 
B)  pharming. 
C)  RNAi. 
D)  DNA microarrray. 
E)  mutagenesis. 
A)  DNA fingerprinting. 
B)  pharming. 
C)  RNAi. 
D)  DNA microarrray. 
E)  mutagenesis.

Question 4

Which of the following statements about the relationship between selectable marker genes and reporter genes is correct?

Accepted Answer

A)  All reporter genes are selectable marker genes. 
B)  All selectable marker genes are reporter genes. 
C)  A selectable marker gene cannot be a reporter gene. 
D)  Both a and b 
E)  None of the above 
A)  All reporter genes are selectable marker genes. 
B)  All selectable marker genes are reporter genes. 
C)  A selectable marker gene cannot be a reporter gene. 
D)  Both a and b 
E)  None of the above

Question 5

Compared with conventional breeding, recombinant DNA technology usually combines _______ genes and takes _______ generations to complete.

Accepted Answer

A)  more; more 
B)  more; about the same number 
C)  more; fewer 
D)  fewer; more 
E)  fewer; fewer 
A)  more; more 
B)  more; about the same number 
C)  more; fewer 
D)  fewer; more 
E)  fewer; fewer

Question 6

The precursor of vitamin A, _______, has been genetically engineered into rice and other plants, in the hope of reducing the incidence of vitamin A deficiency.

Accepted Answer

The answer of The precursor of vitamin A, _______, has...

Question 7

DNA microarrays

Accepted Answer

A)  are used to analyze genomic DNA. 
B)  determine genes that are transcribed. 
C)  use oligoribonucleotides as probes. 
D)  probe noncoding regions of DNA. 
E)  detect proteins that are translated. 
A)  are used to analyze genomic DNA. 
B)  determine genes that are transcribed. 
C)  use oligoribonucleotides as probes. 
D)  probe noncoding regions of DNA. 
E)  detect proteins that are translated.

Question 8

The function of DNA ligase in the generation of recombinant DNA is to

Accepted Answer

A)  cut DNA. 
B)  replicate DNA. 
C)  unwind DNA. 
D)  join DNA fragments by the formation of covalent bonds. 
E)  join DNA fragments noncovalently. 
A)  cut DNA. 
B)  replicate DNA. 
C)  unwind DNA. 
D)  join DNA fragments by the formation of covalent bonds. 
E)  join DNA fragments noncovalently.

Question 9

Genes used to identify transformed cells are called "_______ genes." These genes include one that codes for a protein from a _______.

Accepted Answer

A)  inducible; jellyfish 
B)  reporter; plant 
C)  reporter; jellyfish 
D)  repressible; plant 
E)  repressible; jellyfish 
A)  inducible; jellyfish 
B)  reporter; plant 
C)  reporter; jellyfish 
D)  repressible; plant 
E)  repressible; jellyfish

Question 10

From the list below, select the sequence of steps for inserting a piece of foreign DNA into a plasmid vector, introducing the plasmid into bacteria, and verifying that the plasmid and the foreign gene are present: 
(1) Transform host cells.
(2) Select for the lack of plasmid reporter gene 1 function.
(3) Select for the plasmid reporter gene 2 function.
(4) Digest vector and foreign DNA with a restriction enzyme, which inactivates plasmid reporter gene 1.
(5) Ligate the digested plasmid together with the foreign DNA.

Accepted Answer

A)  4, 5, 1, 3, 2 
B)  4, 5, 1, 2, 3 
C)  1, 3, 4, 2, 5 
D)  3, 2, 1, 4, 5 
E)  1, 3, 2, 5, 4 
A)  4, 5, 1, 3, 2 
B)  4, 5, 1, 2, 3 
C)  1, 3, 4, 2, 5 
D)  3, 2, 1, 4, 5 
E)  1, 3, 2, 5, 4

Question 11

"Sticky ends"

Accepted Answer

A)  are produced by the action of all restriction enzymes. 
B)  form associations with complementary DNA that are very stable. 
C)  are the result of staggered cuts of DNA by restriction enzymes. 
D)  must interact with each other in the formation of recombinant DNA. 
E)  have non-specific base sequences. 
A)  are produced by the action of all restriction enzymes. 
B)  form associations with complementary DNA that are very stable. 
C)  are the result of staggered cuts of DNA by restriction enzymes. 
D)  must interact with each other in the formation of recombinant DNA. 
E)  have non-specific base sequences.

Question 12

An expression vector requires all of the following except

Accepted Answer

A)  genes for ribosomal RNA. 
B)  a reporter gene. 
C)  a promoter of transcription. 
D)  an origin of DNA replication. 
E)  restriction enzyme recognition sequences. 
A)  genes for ribosomal RNA. 
B)  a reporter gene. 
C)  a promoter of transcription. 
D)  an origin of DNA replication. 
E)  restriction enzyme recognition sequences.

Question 13

Which of the following techniques is most likely to make use of reverse transcriptase?

Accepted Answer

A)  Homologous recombination 
B)  DNA microarray 
C)  RNAi 
D)  Mutagenesis 
E)  None of the above 
A)  Homologous recombination 
B)  DNA microarray 
C)  RNAi 
D)  Mutagenesis 
E)  None of the above

Question 14

In the construction of a transgenic cell, a gene that enables growth on arabinose is added to the vector. This gene is being used as a

Accepted Answer

A)  transformation agent. 
B)  selectable marker. 
C)  replicon. 
D)  cloning vector. 
E)  expression vector. 
A)  transformation agent. 
B)  selectable marker. 
C)  replicon. 
D)  cloning vector. 
E)  expression vector.

Question 15

Hosts that are altered by the insertion of recombinant DNA are known as _______ organisms.

Accepted Answer

The answer of Hosts that are altered by the insertion...

Question 16

cDNA libraries

Accepted Answer

A)  are the same as genomic libraries. 
B)  include DNA from non-coding sequences. 
C)  require DNA polymerase to generate. 
D)  require reverse transcriptase to generate. 
E)  likely contain all protein-coding genes. 
A)  are the same as genomic libraries. 
B)  include DNA from non-coding sequences. 
C)  require DNA polymerase to generate. 
D)  require reverse transcriptase to generate. 
E)  likely contain all protein-coding genes.

Question 17

What kind of techniques can be used to study gene expression during development?

Accepted Answer

cDNA libraries can be made fro

Question 18

A lab working with recombinant DNA includes a gene for penicillin resistance as part of the recombinant DNA. In this case, the penicillin resistance gene is acting as a _______ marker.

Accepted Answer

The answer of A lab working with recombinant DNA includes...

Question 19

Which of the following statements about RNAi technology is false?

Accepted Answer

A)  It is based on a natural process for inhibiting translation. 
B)  It was discovered only recently (in the 1990s). 
C)  It has been used as a therapy for macular degeneration. 
D)  It involves doubled-stranded RNA. 
E)  All of the above are true; none is false. 
A)  It is based on a natural process for inhibiting translation. 
B)  It was discovered only recently (in the 1990s). 
C)  It has been used as a therapy for macular degeneration. 
D)  It involves doubled-stranded RNA. 
E)  All of the above are true; none is false.

Question 20

A cDNA clone is

Accepted Answer

A)  mostly cytosine. 
B)  a copy of the DNA identical to the nuclear gene. 
C)  a copy of noncoding DNA. 
D)  a DNA molecule complementary to an mRNA molecule. 
E)  a fragment of DNA inserted into the host chromosome. 
A)  mostly cytosine. 
B)  a copy of the DNA identical to the nuclear gene. 
C)  a copy of noncoding DNA. 
D)  a DNA molecule complementary to an mRNA molecule. 
E)  a fragment of DNA inserted into the host chromosome.

Exam 18: Recombinent Dna and Biotechnology

A plasmid library and a phage library are constructed from the same genetic material. Which of the following statements is true?

Methods developed by the _______ industry were most directly responsible for the development of DNA microarray technologies.

Laura van 't Veer and her colleagues discovered specific differences between cancer patients with good prognoses and those with poor prognoses. The technique most closely associated with the diagnostic tool they invented is

Which of the following statements about the relationship between selectable marker genes and reporter genes is correct?

Compared with conventional breeding, recombinant DNA technology usually combines _______ genes and takes _______ generations to complete.

The precursor of vitamin A, _______, has been genetically engineered into rice and other plants, in the hope of reducing the incidence of vitamin A deficiency.

DNA microarrays

The function of DNA ligase in the generation of recombinant DNA is to

Genes used to identify transformed cells are called "_______ genes." These genes include one that codes for a protein from a _______.

"Sticky ends"

An expression vector requires all of the following except

Which of the following techniques is most likely to make use of reverse transcriptase?

In the construction of a transgenic cell, a gene that enables growth on arabinose is added to the vector. This gene is being used as a

Hosts that are altered by the insertion of recombinant DNA are known as _______ organisms.

cDNA libraries

What kind of techniques can be used to study gene expression during development?

A lab working with recombinant DNA includes a gene for penicillin resistance as part of the recombinant DNA. In this case, the penicillin resistance gene is acting as a _______ marker.

Which of the following statements about RNAi technology is false?

A cDNA clone is

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Filters

Compared with conventional breeding, recombinant DNA technology usually combines _ genes and takes _ generations to complete.

Genes used to identify transformed cells are called "_ genes." These genes include one that codes for a protein from a _.