Question 1

Why are plant cells, as opposed to animal cells, particularly useful as hosts for recombinant DNA?

Accepted Answer

A)  Animal cells cannot be cultured 
B)  Plant cells lack introns. 
C)  Plant cells have more of a capacity to make totipotent stem cells. 
D)  Plant cells have reverse transcriptase. 
E)  None of the above 
A)  Animal cells cannot be cultured 
B)  Plant cells lack introns. 
C)  Plant cells have more of a capacity to make totipotent stem cells. 
D)  Plant cells have reverse transcriptase. 
E)  None of the above

Question 2

Cloning a gene may involve

Accepted Answer

A)  restriction endonucleases and ligase. 
B)  plasmids and bacteriophage $\lambda$. 
C)  transformation or transfection. 
D)  selectable markers and/or reporter genes. 
E)  All of the above 
A)  restriction endonucleases and ligase. 
B)  plasmids and bacteriophage $\lambda$. 
C)  transformation or transfection. 
D)  selectable markers and/or reporter genes. 
E)  All of the above

Question 3

Which of the following enzymes would be used to join two DNA fragments?

Accepted Answer

A)  Reverse transcriptase 
B)  DNA polymerase 
C)  DNA recombinase 
D)  DNA endonuclease 
E)  DNA ligase 
A)  Reverse transcriptase 
B)  DNA polymerase 
C)  DNA recombinase 
D)  DNA endonuclease 
E)  DNA ligase

Question 4

One would most likely use the technique of _______ to determine which particular amino acids made a given protein more able to bind with a receptor.

Accepted Answer

The answer of One would most likely use the technique...

Question 5

Which of the following statements is true?

Accepted Answer

A)  A genomic library constructed from human brain tissue will likely be different than a gene library constructed from human pancreatic tissue. 
B)  A cDNA library constructed from human brain tissue will likely be different than a cDNA library constructed from human pancreatic tissue. 
C)  A cDNA library would be very useful in examining DNA that does not code for mRNA. 
D)  Messenger RNAs have a long life span in the cytoplasm. 
E)  All of the above 
A)  A genomic library constructed from human brain tissue will likely be different than a gene library constructed from human pancreatic tissue. 
B)  A cDNA library constructed from human brain tissue will likely be different than a cDNA library constructed from human pancreatic tissue. 
C)  A cDNA library would be very useful in examining DNA that does not code for mRNA. 
D)  Messenger RNAs have a long life span in the cytoplasm. 
E)  All of the above

Question 6

RNA interference (RNAi) inhibits

Accepted Answer

A)  DNA replication. 
B)  neither transcription nor translation of specific genes. 
C)  recognition of the promoter by RNA polymerase. 
D)  transcription of all genes. 
E)  translation of specific mRNAs. 
A)  DNA replication. 
B)  neither transcription nor translation of specific genes. 
C)  recognition of the promoter by RNA polymerase. 
D)  transcription of all genes. 
E)  translation of specific mRNAs.

Question 7

The crew of an eighteenth century ship sailing from Asia has eaten mostly rice during the voyage. Some of the crew members start experiencing vision difficulties and an increased susceptibility to infection. The most likely cause of these symptoms is a deficiency in

Accepted Answer

A)  vitamin B12. 
B)  vitamin C. 
C)  $\beta$-carotene. 
D)  glyphosate. 
E)  oblimersen. 
A)  vitamin B12. 
B)  vitamin C. 
C)  $\beta$-carotene. 
D)  glyphosate. 
E)  oblimersen.

Question 8

Use the following to answer questions :
The diagram below shows how knockout mice are constructed.  
-When does the insertion of the marker inactivate the function of the target gene?

Accepted Answer

A)  Before arrow 1 
B)  Between arrows 1 and 2 
C)  Between arrows 2 and 3 
D)  Between arrows 3 and 4 
E)  After arrow 4 
A)  Before arrow 1 
B)  Between arrows 1 and 2 
C)  Between arrows 2 and 3 
D)  Between arrows 3 and 4 
E)  After arrow 4

Question 9

Suppose a DNA fragment from a human gene has been cut such that it has sticky ends. A DNA fragment from bacteria with sticky ends complementary to those of the human DNA is added to the mix. What would prevent the complementary ends of the human DNA from rejoining, and thus preventing the formation of a recombinant DNA molecule?

Accepted Answer

A)  DNA ligase 
B)  Restriction endonuclease EcoRI 
C)  Reverse transcriptase 
D)  A cloning vector 
E)  Nothing 
A)  DNA ligase 
B)  Restriction endonuclease EcoRI 
C)  Reverse transcriptase 
D)  A cloning vector 
E)  Nothing

Question 10

Transgenic cells are created via the _______ of recombinant DNA into cells.

Accepted Answer

A)  recombination 
B)  transfection 
C)  ligation 
D)  restriction 
E)  complementation 
A)  recombination 
B)  transfection 
C)  ligation 
D)  restriction 
E)  complementation

Question 11

Restriction enzymes

Accepted Answer

A)  play no role in bacteria. 
B)  cleave DNA at highly specific recognition sequences. 
C)  are inserted into bacteria by bacteriophage. 
D)  are made only by eukaryotic cells. 
E)  add methyl groups to specific DNA sequences. 
A)  play no role in bacteria. 
B)  cleave DNA at highly specific recognition sequences. 
C)  are inserted into bacteria by bacteriophage. 
D)  are made only by eukaryotic cells. 
E)  add methyl groups to specific DNA sequences.

Question 12

Which of the following is a limitation of conventional breeding, as compared with recombinant DNA technology?

Accepted Answer

A)  The limited range of useful genetic variation 
B)  The length of time required by the process 
C)  The lack of precision 
D)  All of the above 
E)  None of the above 
A)  The limited range of useful genetic variation 
B)  The length of time required by the process 
C)  The lack of precision 
D)  All of the above 
E)  None of the above

Question 13

Bacteria are not particularly useful as hosts in studies of eukaryotic gene expression because they

Accepted Answer

A)  are haploid. 
B)  lack the splicing machinery needed to remove introns from eukaryotic mRNA. 
C)  have a small genome size. 
D)  contain plasmids. 
E)  lack genetic markers. 
A)  are haploid. 
B)  lack the splicing machinery needed to remove introns from eukaryotic mRNA. 
C)  have a small genome size. 
D)  contain plasmids. 
E)  lack genetic markers.

Question 14

Which of the following is minimally required for a transgene to be expressed in a eukaryotic host?

Accepted Answer

A)  A promoter, a transcriptional termination site, and a ribosome binding site 
B)  A DNA origin and an antibiotic resistance marker 
C)  Transcription factor binding sites, enhancers, and a poly A-recognition sequence 
D)  An inducible promoter and repressor proteins 
E)  a, c, and d 
A)  A promoter, a transcriptional termination site, and a ribosome binding site 
B)  A DNA origin and an antibiotic resistance marker 
C)  Transcription factor binding sites, enhancers, and a poly A-recognition sequence 
D)  An inducible promoter and repressor proteins 
E)  a, c, and d

Question 15

One of the oldest examples of biotechnology is _______, the cultivation of plants and the domestication of animals.

Accepted Answer

The answer of One of the oldest examples of biotechnology...

Question 16

The use of which of the following allows researchers to examine the patterns of gene expression for a large number of genes simultaneously?

Accepted Answer

A)  Antisense RNA 
B)  RNAi 
C)  DNA microarray 
D)  PCR 
E)  Homologous recombination 
A)  Antisense RNA 
B)  RNAi 
C)  DNA microarray 
D)  PCR 
E)  Homologous recombination

Question 17

RNAi

Accepted Answer

A)  is more effective than antisense RNA in inhibiting translation. 
B)  inhibits transcription in eukaryotes. 
C)  is produced only by viruses. 
D)  requires other proteins to modify the RNAi. 
E)  Both a and d 
A)  is more effective than antisense RNA in inhibiting translation. 
B)  inhibits transcription in eukaryotes. 
C)  is produced only by viruses. 
D)  requires other proteins to modify the RNAi. 
E)  Both a and d

Question 18

In the construction of an expression vector, a signal sequence

Accepted Answer

A)  allows for the expression of a gene in a particular tissue only. 
B)  allows the expression of a gene to be turned on or off. 
C)  allows the gene product to be directed to an appropriate location. 
D)  makes a gene responsive to hormonal stimulation. 
E)  None of the above 
A)  allows for the expression of a gene in a particular tissue only. 
B)  allows the expression of a gene to be turned on or off. 
C)  allows the gene product to be directed to an appropriate location. 
D)  makes a gene responsive to hormonal stimulation. 
E)  None of the above

Question 19

Use the following to answer questions:
A plasmid has genes that confer resistance to both ampicillin and tetracycline. Foreign DNA is inserted at a restriction enzyme recognition site that lies within the ampicillin gene.
-After host cells have been given the opportunity to take up the plasmid, which of the following types of cells should be the most rare?

Accepted Answer

A)  Bacteria with no plasmid and no foreign DNA 
B)  Bacteria with the foreign DNA, but no plasmid 
C)  Bacteria with the recombinant plasmid 
D)  Bacteria with the nonrecombinant plasmid 
E)  All types should appear with approximately the same frequency. 
A)  Bacteria with no plasmid and no foreign DNA 
B)  Bacteria with the foreign DNA, but no plasmid 
C)  Bacteria with the recombinant plasmid 
D)  Bacteria with the nonrecombinant plasmid 
E)  All types should appear with approximately the same frequency.

Question 20

Composting is an example of

Accepted Answer

A)  pharming. 
B)  bioremediation. 
C)  biotechnology. 
D)  Both a and b 
E)  Both b and c 
A)  pharming. 
B)  bioremediation. 
C)  biotechnology. 
D)  Both a and b 
E)  Both b and c

Exam 18: Recombinent Dna and Biotechnology

Why are plant cells, as opposed to animal cells, particularly useful as hosts for recombinant DNA?

Cloning a gene may involve

Which of the following enzymes would be used to join two DNA fragments?

One would most likely use the technique of _______ to determine which particular amino acids made a given protein more able to bind with a receptor.

Which of the following statements is true?

RNA interference (RNAi) inhibits

The crew of an eighteenth century ship sailing from Asia has eaten mostly rice during the voyage. Some of the crew members start experiencing vision difficulties and an increased susceptibility to infection. The most likely cause of these symptoms is a deficiency in

Use the following to answer questions : The diagram below shows how knockout mice are constructed. -When does the insertion of the marker inactivate the function of the target gene?

Transgenic cells are created via the _______ of recombinant DNA into cells.

Restriction enzymes

Which of the following is a limitation of conventional breeding, as compared with recombinant DNA technology?

Bacteria are not particularly useful as hosts in studies of eukaryotic gene expression because they

Which of the following is minimally required for a transgene to be expressed in a eukaryotic host?

One of the oldest examples of biotechnology is _______, the cultivation of plants and the domestication of animals.

The use of which of the following allows researchers to examine the patterns of gene expression for a large number of genes simultaneously?

RNAi

In the construction of an expression vector, a signal sequence

Composting is an example of

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