Exam 18: Recombinent Dna and Biotechnology

Suppose that your lab assistant has cloned gene X into yeast and confirmed that the recombinant DNA molecule is present in the yeast cells. However, the yeast cell is unable to synthesize X protein. Suggest why this part of the experiment is not working and what modifica­tions are needed in the cloning procedure.

Recombinant DNA technology has produced medically useful products. Most of these products are _______ that are normally present in low amounts in animals and are difficult to _______; _______ vectors were used to obtain these products in large amounts.

The enzyme that catalyzes the joining of DNA fragments is known as _______.

Reporter genes include genes for

Which of the following characteristics makes plasmids useful as a vector?

Which of the following statements about recombinant DNA is true?

What is the major difference between using green fluorescent protein versus using an antibiotic resistance gene as a reporter gene?

Found in Agrobacterium tumefaciens, the _______ permits the bacterium to infect plants. It is often used as a vector in plant recombinant DNA technology.

A disadvantage of recombinant DNA technology is that it

A plasmid

Gene expression can be inhibited by

Suppose that you wanted to express a protein from animal cells using recombinant DNA technology. Why might you prefer to use yeast as the host rather than E. coli?

A toxin gene from the bacterium _______, which has been genetically engineered into several crop plants, allows these plants to produce their own insecticides.

Which of the following can be used to inhibit translation of a specific gene?

"Pharming" is a term that describes

Which of the following was used to clean up the oil after the Exxon Valdez spill?

An important enzyme, called _______, is essential for synthesizing cDNA from mRNA.

Pharming is

What was the original source of the green fluorescent protein?

What does the "c" in "cDNA library" stand for?