Question 1

Which of the following statements about mouse embryonic stem cells is true?

Accepted Answer

A)  They can serve as hosts for transfection studies using homologous recombination. 
B)  They are unspecialized. 
C)  They can be used in knockout studies. 
D)  All of the above 
E)  None of the above 
A)  They can serve as hosts for transfection studies using homologous recombination. 
B)  They are unspecialized. 
C)  They can be used in knockout studies. 
D)  All of the above 
E)  None of the above D

Question 2

Which of the following characteristics would not be useful in a vector?

Accepted Answer

A)  A recognition sequence for a restriction enzyme 
B)  Large size relative to the host chromosomes 
C)  The ability to replicate independently inside the host cell 
D)  A reporter gene 
E)  An origin of replication 
A)  A recognition sequence for a restriction enzyme 
B)  Large size relative to the host chromosomes 
C)  The ability to replicate independently inside the host cell 
D)  A reporter gene 
E)  An origin of replication B

Question 3

Oblimerson, a drug developed against cancer, is one example of the uses of _______ technology.

Accepted Answer

A)  RNAi 
B)  homologous recombination 
C)  microarray 
D)  antisense RNA 
E)  bioremediation 
A)  RNAi 
B)  homologous recombination 
C)  microarray 
D)  antisense RNA 
E)  bioremediation D

Question 4

In a genomic library of frog DNA in E. coli bacteria,

Accepted Answer

A)  all bacterial cells have the same sequences of frog DNA. 
B)  all bacterial cells have different sequences of frog DNA. 
C)  each bacterial cell has a random fragment of frog DNA. 
D)  each bacterial cell has many fragments of frog DNA. 
E)  the frog DNA is transcribed into mRNA in the bacterial cells. 
A)  all bacterial cells have the same sequences of frog DNA. 
B)  all bacterial cells have different sequences of frog DNA. 
C)  each bacterial cell has a random fragment of frog DNA. 
D)  each bacterial cell has many fragments of frog DNA. 
E)  the frog DNA is transcribed into mRNA in the bacterial cells.

Question 5

The production of many copies of a particular gene is called

Accepted Answer

A)  transfection. 
B)  restriction. 
C)  cloning. 
D)  annealing. 
E)  inducing. 
A)  transfection. 
B)  restriction. 
C)  cloning. 
D)  annealing. 
E)  inducing.

Question 6

Possession of which feature is not desirable in a vector for gene cloning?

Accepted Answer

A)  An origin of DNA replication 
B)  Genetic markers for the presence of the vector 
C)  Many recognition sequences for the restriction enzyme to be used 
D)  One recognition sequence each for one or more different restriction enzymes 
E)  Genes other than the target for transfection 
A)  An origin of DNA replication 
B)  Genetic markers for the presence of the vector 
C)  Many recognition sequences for the restriction enzyme to be used 
D)  One recognition sequence each for one or more different restriction enzymes 
E)  Genes other than the target for transfection

Question 7

A researcher inserts a DNA segment at the BamHI recognition site within a plasmid; this site is located within the tetracycline resistance gene. This plasmid also has a gene for ampicillin resistance. Following DNA transformation, the researcher must differentiate the bacteria that have taken up the recombinant DNA from those that have taken up either the DNA segment only or intact plasmids. In doing so, the researcher should select the bacteria that

Accepted Answer

A)  will grow on ampicillin but are sensitive to tetracycline. 
B)  are sensitive to both antibiotics. 
C)  are resistant to both antibiotics. 
D)  will grow on tetracycline but are sensitive to ampicillin. 
E)  grow only on an enriched medium. 
A)  will grow on ampicillin but are sensitive to tetracycline. 
B)  are sensitive to both antibiotics. 
C)  are resistant to both antibiotics. 
D)  will grow on tetracycline but are sensitive to ampicillin. 
E)  grow only on an enriched medium.

Question 8

The two enzymes that are most important in the construction of recombinant DNA are _______ and _______.

Accepted Answer

A)  restriction enzymes; reverse transcriptase 
B)  restriction enzymes; DNA ligase 
C)  reverse transcriptase; DNA polymerase 
D)  TPA; reverse transcriptase 
E)  cytochrome oxidase; DNA polymerase 
A)  restriction enzymes; reverse transcriptase 
B)  restriction enzymes; DNA ligase 
C)  reverse transcriptase; DNA polymerase 
D)  TPA; reverse transcriptase 
E)  cytochrome oxidase; DNA polymerase

Question 9

Complementary RNA

Accepted Answer

A)  forms hybrids with mRNA to prevent translation. 
B)  inhibits transcription. 
C)  blocks DNA replication. 
D)  is sense RNA. 
E)  blocks translation by joining with rRNA. 
A)  forms hybrids with mRNA to prevent translation. 
B)  inhibits transcription. 
C)  blocks DNA replication. 
D)  is sense RNA. 
E)  blocks translation by joining with rRNA.

Question 10

EcoRI makes staggered cuts when it cleaves DNA, creating single-stranded tails called "sticky ends." These ends can form a complementary base pair. In order for this to happen, which of the following conditions is necessary?

Accepted Answer

A)  The presence of specific helicases 
B)  High enough temperatures 
C)  Methyl groups at each end 
D)  Sufficiently low temperature 
E)  None of the above 
A)  The presence of specific helicases 
B)  High enough temperatures 
C)  Methyl groups at each end 
D)  Sufficiently low temperature 
E)  None of the above

Question 11

Transposon mutagenesis

Accepted Answer

A)  works only in animals. 
B)  is highly gene specific. 
C)  can create many mutants for screening. 
D)  results from homologous recombination. 
E)  Both b and d 
A)  works only in animals. 
B)  is highly gene specific. 
C)  can create many mutants for screening. 
D)  results from homologous recombination. 
E)  Both b and d

Question 12

The green fluorescent protein, which emits visible light when exposed to UV light, is commonly used as a(n) _______ in recombinant DNA technology.

Accepted Answer

The answer of The green fluorescent protein, which emits visible...

Question 13

Which of the following properties would distinguish an expression vector from typical vectors?

Accepted Answer

A)  Reverse transcriptase 
B)  A recognition sequence for a restriction enzyme 
C)  Transcription binding sites 
D)  A reporter gene 
E)  The ability to replicate independently within the host 
A)  Reverse transcriptase 
B)  A recognition sequence for a restriction enzyme 
C)  Transcription binding sites 
D)  A reporter gene 
E)  The ability to replicate independently within the host

Question 14

For a prokaryotic vector to be propagated in a host bacterial cell, the vector needs

Accepted Answer

A)  an origin of replication. 
B)  telomeres. 
C)  centromeres. 
D)  drug-resistance genes. 
E)  reporter genes. 
A)  an origin of replication. 
B)  telomeres. 
C)  centromeres. 
D)  drug-resistance genes. 
E)  reporter genes.

Question 15

Use the following to answer questions:
A plasmid has genes that confer resistance to both ampicillin and tetracycline. Foreign DNA is inserted at a restriction enzyme recognition site that lies within the ampicillin gene.
-A cell transformed with the resulting recombinant plasmid should be _______ to ampicillin and _______ to tetracycline.

Accepted Answer

A)  resistant; resistant 
B)  resistant; sensitive 
C)  resistant; complementary 
D)  sensitive; resistant 
E)  sensitive; complementary 
A)  resistant; resistant 
B)  resistant; sensitive 
C)  resistant; complementary 
D)  sensitive; resistant 
E)  sensitive; complementary

Question 16

An important vector for the manipulation of plant genes comes from the bacterium A. tumefaciens and is called

Accepted Answer

A)  an EcoRI plasmid. 
B)  $\lambda$ bacteriophage. 
C)  BamHI. 
D)  a Ti plasmid. 
E)  None of the above 
A)  an EcoRI plasmid. 
B)  $\lambda$ bacteriophage. 
C)  BamHI. 
D)  a Ti plasmid. 
E)  None of the above

Question 17

Hybridization is a useful technique in biotechnology. Describe three ways hybridization is used experimentally in DNA recombinant technology.

Accepted Answer

Hybridization of complementary base pair

Question 18

Which of the following is an example of an inducible promoter?

Accepted Answer

A)  A promoter that responds only in liver tissue 
B)  A promoter that responds only when juvenile hormone is present 
C)  A promoter that responds all the time, regardless of conditions 
D)  A promoter that possess signal sequences 
E)  A promoter that produces erythropoietin. 
A)  A promoter that responds only in liver tissue 
B)  A promoter that responds only when juvenile hormone is present 
C)  A promoter that responds all the time, regardless of conditions 
D)  A promoter that possess signal sequences 
E)  A promoter that produces erythropoietin.

Question 19

Biologists use _______ technology to examine the patterns of expression of very large numbers of genes.

Accepted Answer

DNA chip (

Question 20

Which of the following necessarily makes use of reverse transcriptase?

Accepted Answer

A)  Southern blotting 
B)  Construction of a gene library 
C)  Construction of a cDNA library 
D)  Gene knockout studies 
E)  Pharming 
A)  Southern blotting 
B)  Construction of a gene library 
C)  Construction of a cDNA library 
D)  Gene knockout studies 
E)  Pharming

Exam 18: Recombinent Dna and Biotechnology

Which of the following statements about mouse embryonic stem cells is true?

Which of the following characteristics would not be useful in a vector?

Oblimerson, a drug developed against cancer, is one example of the uses of _______ technology.

In a genomic library of frog DNA in E. coli bacteria,

The production of many copies of a particular gene is called

Possession of which feature is not desirable in a vector for gene cloning?

The two enzymes that are most important in the construction of recombinant DNA are _______ and _______.

Complementary RNA

EcoRI makes staggered cuts when it cleaves DNA, creating single-stranded tails called "sticky ends." These ends can form a complementary base pair. In order for this to happen, which of the following conditions is necessary?

Transposon mutagenesis

The green fluorescent protein, which emits visible light when exposed to UV light, is commonly used as a(n) _______ in recombinant DNA technology.

Which of the following properties would distinguish an expression vector from typical vectors?

For a prokaryotic vector to be propagated in a host bacterial cell, the vector needs

An important vector for the manipulation of plant genes comes from the bacterium A. tumefaciens and is called

Hybridization is a useful technique in biotechnology. Describe three ways hybridization is used experimentally in DNA recombinant technology.

Which of the following is an example of an inducible promoter?

Biologists use _______ technology to examine the patterns of expression of very large numbers of genes.

Which of the following necessarily makes use of reverse transcriptase?

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