Question 1

Plant scientists have successfully engineered some broadleaf crop plants to be resistant to the application of glyphosate.Glyphosate works by

Accepted Answer

A) inhibiting an enzyme known as RNA polymerase,which is required for aromatic amino acid production. 
B) inhibiting an enzyme known as DNA polymerase II,which is required for aromatic amino acid production. 
C) inhibiting an enzyme known as EPSP synthetase,which is required for aromatic amino acid production. 
D) inhibiting an enzyme known as EPSP synthetase,which is required for uncoupling the mRNA from ribosomes during protein synthesis. 
A) inhibiting an enzyme known as RNA polymerase,which is required for aromatic amino acid production. 
B) inhibiting an enzyme known as DNA polymerase II,which is required for aromatic amino acid production. 
C) inhibiting an enzyme known as EPSP synthetase,which is required for aromatic amino acid production. 
D) inhibiting an enzyme known as EPSP synthetase,which is required for uncoupling the mRNA from ribosomes during protein synthesis.

Question 2

Automated enzymatic sequencing employs

Accepted Answer

A) different colored fluorescent dyes conjugated to dideoxy terminators and capillary gel electrophoresis. 
B) radioactively-labeled dideoxy terminators and capillary gel electrophoresis. 
C) different colored fluorescent dyes conjugated to dideoxy terminators and slab gel electrophoresis. 
D) radioactively-labeled dideoxy terminators and slab gel electrophoresis. 
A) different colored fluorescent dyes conjugated to dideoxy terminators and capillary gel electrophoresis. 
B) radioactively-labeled dideoxy terminators and capillary gel electrophoresis. 
C) different colored fluorescent dyes conjugated to dideoxy terminators and slab gel electrophoresis. 
D) radioactively-labeled dideoxy terminators and slab gel electrophoresis.

Question 3

The polymerase chain reaction (PCR)includes all of the following steps except

Accepted Answer

A) denaturation of primers and the DNA fragment to be amplified. 
B) anealing of primers to the complementary sequences on the DNA. 
C) primer extension with DNA polymerase. 
D) cycle repetition. 
E) addition of new Taq polymerase after each cyclE. 
A) denaturation of primers and the DNA fragment to be amplified. 
B) anealing of primers to the complementary sequences on the DNA. 
C) primer extension with DNA polymerase. 
D) cycle repetition. 
E) addition of new Taq polymerase after each cyclE.

Question 4

The most recent techniques developed in the biological sciences allow the manipulation of DNA with the ultimate goal of intervening directly with the _______ fate of organisms.

Accepted Answer

A) genetic 
B) developmental 
C) adult 
D) mitotic 
A) genetic 
B) developmental 
C) adult 
D) mitotic

Question 5

A plasmid used to carry genes into crop plants is

Accepted Answer

A) Ti. 
B) glyphosphate. 
C) somatotropin. 
D) recombinant DNA. 
E) Roundup. 
A) Ti. 
B) glyphosphate. 
C) somatotropin. 
D) recombinant DNA. 
E) Roundup.

Question 6

An insecticidal protein has been discovered in a bacterium known as

Accepted Answer

A) Escherichia coli. 
B) Agrobacterium tumefaciens. 
C) Bacillus thuringinensis. 
D) Aspergillus sp. 
A) Escherichia coli. 
B) Agrobacterium tumefaciens. 
C) Bacillus thuringinensis. 
D) Aspergillus sp.

Question 7

All of the following involve molecular biology techniques except

Accepted Answer

A) cutting and rearranging the DNA. 
B) using restriction enzymes to cut specific sequences of DNA. 
C) cloning the genes into the host organism. 
D) using any cell as a vector. 
A) cutting and rearranging the DNA. 
B) using restriction enzymes to cut specific sequences of DNA. 
C) cloning the genes into the host organism. 
D) using any cell as a vector.

Question 8

Most genetic engineering experiments include four stages.Which of the following is not one of them?

Accepted Answer

A) cleaving the source DNA 
B) production of recombinant DNA 
C) cloning copies of the recombinanats 
D) screening the cloned copies for the desired gene 
E) integration of the entire bacterial chromosome 
A) cleaving the source DNA 
B) production of recombinant DNA 
C) cloning copies of the recombinanats 
D) screening the cloned copies for the desired gene 
E) integration of the entire bacterial chromosome

Question 9

Viruses and bacterial plasmids are used as ______ to insert foreign DNA into host cells and create recombinant genomes.

Accepted Answer

A) clones 
B) vectors 
C) endonucleases 
D) subunits 
A) clones 
B) vectors 
C) endonucleases 
D) subunits

Question 10

A commercially significant human protein now produced in bacteria is

Accepted Answer

A) hemoglobin. 
B) gamma globulins. 
C) AZT. 
D) human insulin. 
E) HIV vaccinE. 
A) hemoglobin. 
B) gamma globulins. 
C) AZT. 
D) human insulin. 
E) HIV vaccinE.

Exam 17: Biotechnology

Plant scientists have successfully engineered some broadleaf crop plants to be resistant to the application of glyphosate.Glyphosate works by

Automated enzymatic sequencing employs

The polymerase chain reaction (PCR)includes all of the following steps except

The most recent techniques developed in the biological sciences allow the manipulation of DNA with the ultimate goal of intervening directly with the _______ fate of organisms.

A plasmid used to carry genes into crop plants is

An insecticidal protein has been discovered in a bacterium known as

All of the following involve molecular biology techniques except

Most genetic engineering experiments include four stages.Which of the following is not one of them?

Viruses and bacterial plasmids are used as ______ to insert foreign DNA into host cells and create recombinant genomes.

A commercially significant human protein now produced in bacteria is

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