Exam 17: Biotechnology

You are performing a Southern blot analysis.To obtain a probe,you digest the above piece of DNA with Pst I and isolate a 2.6 kb Pst I fragment.You radiolabel the fragment and use it to probe a Southern blot of the above DNA that has been digested with Eco RI.Which of the following DNA fragments does your probe detect?

Restriction enzymes recognize DNA sequences that are

Bam HI is a Type II restriction enzyme that recognizes the sequence 5' GGATCC 3',and cuts between the two Gs,leaving a 5' overhang.If you were to digest DNA with Bam HI,what would be the sequence of the overhanging sticky end of the complimentary strand?

Your research project involves the characterization of a signal transduction pathway,and you would like to identify proteins that may interact with one of the pathway components.Which of the following libraries could you screen for this purpose?

Technique used to analyze DNA products after restriction enzyme digestion;"fingerprints."

Small circular,extrachromosomal DNA segments are known as ________.

The most common way to follow bacterial transformation with a plasmid is by

Small,circular auxiliary DNA molecules of bacteria which are commonly used in biotechnology are referred to as

Scientists can distinguish between DNA of different individuals,thus making this information useful in criminal investigations.The technique used is called

Current vectors used for gene therapy insert into the genome as a random event.All of the following are potential problems that may result from this method of integration except

The _________ from Agrobacterium tumefaciens is used in genetic engineering involving crop plant genes.

If you were to digest this fragment of DNA with Pst I,how many pieces of DNA would you obtain?

The procedure for producing a line of genetically identical cells from a single "altered" cell is called _______.

When electrical current is applied during a gel electrophoresis procedure,the DNA fragments are separated by

Restriction enzymes are

Organisms produced by moving genes of one species to another;desired characteristics such as disease resistance or increased size are transferred.

Crop plants have been protected from certain insects by the addition of a gene for a specific insect toxin from the bacterium

Some food crops are difficult to grow for a variety of reasons.For example,strawberries are easily susceptible to frost damage.How could one use genetic engineering techniques help to overcome this type of problem,and which type of animal or plant would one look to for help?

A vector "carries" ______________ into a host cell.

You are hired as a scientific consultant for a popular television show about forensic analysis of crime scene evidence.You are told that in an upcoming scene,investigators will compare the DNA profiles of a known suspect and an unknown biological sample collected from the victim.You are asked to write up a brief explanation of the primary technique required for discussion in the scene.Which technique should you write about?