Question 1

In a manual enzymatic DNA sequencing reaction,the nucleotide ddT is incorporated

Accepted Answer

A) at every T position in the synthesized DNA strand;the reaction is terminated when all T positions have been filled with ddT. 
B) randomly at a single T position in the synthesized DNA strand,leading to termination of the reaction. 
C) at several T positions in the synthesized strand,leading to termination of the reaction. 
D) randomly at a A position in the template DNA strand,leading to termination of the reaction. 
A) at every T position in the synthesized DNA strand;the reaction is terminated when all T positions have been filled with ddT. 
B) randomly at a single T position in the synthesized DNA strand,leading to termination of the reaction. 
C) at several T positions in the synthesized strand,leading to termination of the reaction. 
D) randomly at a A position in the template DNA strand,leading to termination of the reaction. B

Question 2

DNA can be cleaved at specific sites,generating smaller DNA fragments with short single-stranded ends.The chemical tool used to cleave the DNA is called a(n)

Accepted Answer

A) hybridization enzyme. 
B) complementary enzyme. 
C) restriction enzyme. 
D) methylating enzyme. 
E) endonucleasE. 
A) hybridization enzyme. 
B) complementary enzyme. 
C) restriction enzyme. 
D) methylating enzyme. 
E) endonucleasE. C

Question 3

Which of the following is not an example of reverse genetics?

Accepted Answer

A)  generation and analysis of a knockout mouse 
B)  RNA interference of a gene in C. elegans 
C)  generation of a transgenic fly that overexpresses an altered form of a gene 
D)  identification of the gene responsible for particular mutant phenotype 
A)  generation and analysis of a knockout mouse 
B)  RNA interference of a gene in C. elegans 
C)  generation of a transgenic fly that overexpresses an altered form of a gene 
D)  identification of the gene responsible for particular mutant phenotype D

Question 4

Technique used to amplify a desired piece of DNA.

Accepted Answer

A) sticky ends 
B) RFLP 
C) PCR 
D) plasmids 
E) transgenic organisms 
A) sticky ends 
B) RFLP 
C) PCR 
D) plasmids 
E) transgenic organisms

Question 5

You are performing a yeast two hybrid screen to identify protein-protein interactions.Your bait is a 25 kD protein fused to the GAL4 DNA-binding domain.Which of the following best describes the nature of the library that you are using?

Accepted Answer

A) The library contains the prey fused to the GAL4 activation domain. 
B) The library contains the reporter gene fused to the GAL4 activation domain. 
C) The library contains the bait fused to the GAL4 activation domain. 
D) The library contains the bait fused to the reporter gene,which is upstream of the GAL4 activation domain. 
A) The library contains the prey fused to the GAL4 activation domain. 
B) The library contains the reporter gene fused to the GAL4 activation domain. 
C) The library contains the bait fused to the GAL4 activation domain. 
D) The library contains the bait fused to the reporter gene,which is upstream of the GAL4 activation domain.

Question 6

If the lacZ gene is functional it produces an enzyme known as _____________,which allows the bacterial cell to metabolize the sugar,X-gal.

Accepted Answer

A) beta-glucosidase 
B) beta-xylosidase 
C) gamma-endonuclease 
D) alpha-galactosidase 
E) beta-galactosidase 
A) beta-glucosidase 
B) beta-xylosidase 
C) gamma-endonuclease 
D) alpha-galactosidase 
E) beta-galactosidase

Question 7

A ______ vaccine is produced by using only a part of the viral genome.

Accepted Answer

A) immunization 
B) subunit 
C) bacterial 
D) prokaryotic 
A) immunization 
B) subunit 
C) bacterial 
D) prokaryotic

Question 8

A vaccine that depends on the cellular immune response of killer T cells is called a

Accepted Answer

A) subunit vaccine. 
B) DNA vaccine. 
C) cellular response vaccine. 
D) T cell vaccine. 
A) subunit vaccine. 
B) DNA vaccine. 
C) cellular response vaccine. 
D) T cell vaccine.

Question 9

What is the environmental benefit of the Enviropig?

Accepted Answer

A) increased production of phosphate in pig waste 
B) reduction of nutritional needs,thereby saving grains 
C) reduction of phosphate excretion in pig waste 
D) increased fat and flavor of pork 
A) increased production of phosphate in pig waste 
B) reduction of nutritional needs,thereby saving grains 
C) reduction of phosphate excretion in pig waste 
D) increased fat and flavor of pork

Question 10

You are trying to clone a piece of DNA into a plasmid vector that exhibits blue-white selection.Following ligation and transformation,you plate the host cells onto several plates containing the appropriate selective media.The next day you examine the bacterial colonies that grew.Which of the following plates should you select to proceed with the analysis of possible transformants?

Accepted Answer

A) 3 white colonies,50 blue colonies 
B) 97 white colonies,105 blue colonies 
C) 43 white colonies,6 blue colonies 
D) 238 blue colonies 
A) 3 white colonies,50 blue colonies 
B) 97 white colonies,105 blue colonies 
C) 43 white colonies,6 blue colonies 
D) 238 blue colonies

Question 11

Some of the useful applications of genetic engineering include all of the following except

Accepted Answer

A) bacteria that can digest oil in an oil spill. 
B) growing synthetic cotton. 
C) manufacturing biopolymers. 
D) using PCR to study ancient fossils. 
E) to clone the perfect human being. 
A) bacteria that can digest oil in an oil spill. 
B) growing synthetic cotton. 
C) manufacturing biopolymers. 
D) using PCR to study ancient fossils. 
E) to clone the perfect human being.

Question 12

Which of the following vectors is the best choice to clone a 23 kb genomic DNA fragment?

Accepted Answer

A) plasmid vector 
B) phage vector 
C) YAC 
D) BAC 
A) plasmid vector 
B) phage vector 
C) YAC 
D) BAC

Question 13

The ends of DNA fragments produced by the same restriction enzyme can be joined together by another enzyme,

Accepted Answer

A) DNA polymerase. 
B) DNA methylase. 
C) DNA synthetase. 
D) DNA ligase. 
E) DNA primasE. 
A) DNA polymerase. 
B) DNA methylase. 
C) DNA synthetase. 
D) DNA ligase. 
E) DNA primasE.

Question 14

In genetic engineering experiments,a common way of identifying a transformed cell is by using a "probe," which is a

Accepted Answer

A) specific complimentary double stranded DNA. 
B) specific complimentary double stranded RNA. 
C) specific complimentary single stranded DNA. 
D) specific complimentary single stranded RNA. 
A) specific complimentary double stranded DNA. 
B) specific complimentary double stranded RNA. 
C) specific complimentary single stranded DNA. 
D) specific complimentary single stranded RNA.

Question 15

Single-stranded complementary tails that are produced by restriction digestion are called ______ ends.

Accepted Answer

A) sticky 
B) orphaned 
C) vectors 
D) 5' 
A) sticky 
B) orphaned 
C) vectors 
D) 5'

Question 16

Agrobacterium is more difficult to use in genetic engineering procedures conducted on cereals such as corn,rice,and wheat because

Accepted Answer

A) it does not infect these types of plants. 
B) it does not contain the correct restriction enzymes necessary for insertion. 
C) its plasmids have been altered to be used only in broadleaf plants. 
D) it interferes with the nitrogen fixing capabilities of narrow leaf plants. 
A) it does not infect these types of plants. 
B) it does not contain the correct restriction enzymes necessary for insertion. 
C) its plasmids have been altered to be used only in broadleaf plants. 
D) it interferes with the nitrogen fixing capabilities of narrow leaf plants.

Question 17

If you were to perform a double digest of this DNA using Bam HI and Eco RI,what is the size of the DNA fragment that would migrate the fastest on an agarose gel?

Accepted Answer

A) 803 kb 
B) 1038 kb 
C) 2283 kb 
D) 6850 kb 
A) 803 kb 
B) 1038 kb 
C) 2283 kb 
D) 6850 kb

Question 18

To generate a transgenic mouse,one would

Accepted Answer

A) introduce the knocked-out gene into a blastocyst embryo,isolate the embryonic stem cells,implant the embryonic stem cells into a pseudopregnant female. 
B) introduce the knocked-out gene into embryonic stem cells,isolate the cells from a pseudopregnant female,inject the transformed cells into a blastocyst stage embryo. 
C) introduce the knocked-out gene into embryonic stem cells,implant the embryonic stem cells into a pseudopregnant female. 
D) introduce the knocked-out gene into embryonic stem cells,inject the embryonic stem cells into a blastocyst stage embryo,implant the injected blastocyst into a pseudopregnant female. 
A) introduce the knocked-out gene into a blastocyst embryo,isolate the embryonic stem cells,implant the embryonic stem cells into a pseudopregnant female. 
B) introduce the knocked-out gene into embryonic stem cells,isolate the cells from a pseudopregnant female,inject the transformed cells into a blastocyst stage embryo. 
C) introduce the knocked-out gene into embryonic stem cells,implant the embryonic stem cells into a pseudopregnant female. 
D) introduce the knocked-out gene into embryonic stem cells,inject the embryonic stem cells into a blastocyst stage embryo,implant the injected blastocyst into a pseudopregnant female.

Question 19

A researcher identified a new enzyme and is interested in determining the mRNA expression pattern of this gene.Which of the following techniques would accomplish this goal?

Accepted Answer

A) Southern blot 
B) northern blot 
C) western blot 
D) eastern blot 
A) Southern blot 
B) northern blot 
C) western blot 
D) eastern blot

Question 20

Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another,using molecular cloning techniques?

Accepted Answer

A) restriction fragment length polymorphism data 
B) sequence analysis 
C) Southern blot data 
D) restriction maps of the plasmids 
A) restriction fragment length polymorphism data 
B) sequence analysis 
C) Southern blot data 
D) restriction maps of the plasmids

Exam 17: Biotechnology

In a manual enzymatic DNA sequencing reaction,the nucleotide ddT is incorporated

DNA can be cleaved at specific sites,generating smaller DNA fragments with short single-stranded ends.The chemical tool used to cleave the DNA is called a(n)

Which of the following is not an example of reverse genetics?

Technique used to amplify a desired piece of DNA.

You are performing a yeast two hybrid screen to identify protein-protein interactions.Your bait is a 25 kD protein fused to the GAL4 DNA-binding domain.Which of the following best describes the nature of the library that you are using?

If the lacZ gene is functional it produces an enzyme known as _____________,which allows the bacterial cell to metabolize the sugar,X-gal.

A ______ vaccine is produced by using only a part of the viral genome.

A vaccine that depends on the cellular immune response of killer T cells is called a

What is the environmental benefit of the Enviropig?

Some of the useful applications of genetic engineering include all of the following except

Which of the following vectors is the best choice to clone a 23 kb genomic DNA fragment?

The ends of DNA fragments produced by the same restriction enzyme can be joined together by another enzyme,

In genetic engineering experiments,a common way of identifying a transformed cell is by using a "probe," which is a

Single-stranded complementary tails that are produced by restriction digestion are called ______ ends.

Agrobacterium is more difficult to use in genetic engineering procedures conducted on cereals such as corn,rice,and wheat because

If you were to perform a double digest of this DNA using Bam HI and Eco RI,what is the size of the DNA fragment that would migrate the fastest on an agarose gel?

To generate a transgenic mouse,one would

A researcher identified a new enzyme and is interested in determining the mRNA expression pattern of this gene.Which of the following techniques would accomplish this goal?

Which of the following types of information would be most useful in an effort to move a DNA fragment from one plasmid vector to another,using molecular cloning techniques?

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