Question 1

Which of the following is correct regarding allogeneic stem cell transplant and ABO antigens?

Accepted Answer

A) ABO antigens do not need to be matched. 
B) ABO antigens are strongly expressed on stem cells. 
C) The O group patient should not receive stem cells from A or B blood group donors. 
D) ABO antigens should always be matched between donor and recipient. 
A) ABO antigens do not need to be matched. 
B) ABO antigens are strongly expressed on stem cells. 
C) The O group patient should not receive stem cells from A or B blood group donors. 
D) ABO antigens should always be matched between donor and recipient.

Question 2

Which HSC quantitation method would be optimal for verification of late-stage engraftment?

Accepted Answer

A) Manual count of MNCs 
B) CD34 enumeration by flow cytometry 
C) Cell culture for CFUs 
D) Bone marrow exam for stem cells 
A) Manual count of MNCs 
B) CD34 enumeration by flow cytometry 
C) Cell culture for CFUs 
D) Bone marrow exam for stem cells

Question 3

The most important antigens that need to be matched for a successful outcome in stem cell transplants are:

Accepted Answer

A) ABO 
B) Rh 
C) HLA 
D) Ii 
A) ABO 
B) Rh 
C) HLA 
D) Ii

Question 4

Explain the significance of HLA and ABO antigen compatibility in stem cell transplantation.

Accepted Answer

Compatibility between donor and recipien

Question 5

Graft-versus-host disease results from which of the following?

Accepted Answer

A) Donor T cells initiating an immune response against host recipient cells 
B) Secondary CMV infection 
C) Recipient T cells initiating an immune response against donor cells 
D) Tumor infiltration in MALT tissue 
A) Donor T cells initiating an immune response against host recipient cells 
B) Secondary CMV infection 
C) Recipient T cells initiating an immune response against donor cells 
D) Tumor infiltration in MALT tissue

Question 6

What is the single most important factor considered in donor selection for an allogeneic transplant?

Accepted Answer

A) HLA compatibility with the patient 
B) ABO compatibility with the patient 
C) CMV status of the donor 
D) HLA and ABO compatibility with the recipient 
A) HLA compatibility with the patient 
B) ABO compatibility with the patient 
C) CMV status of the donor 
D) HLA and ABO compatibility with the recipient

Question 7

One disadvantage of using autologous stem cells for treatment in a leukemia patient is:

Accepted Answer

A) The graft-versus-leukemia effect is not possible. 
B) There is increased risk for graft-versus-host disease. 
C) There is not a need for immunosuppression. 
D) The yield of stem cells is low. 
A) The graft-versus-leukemia effect is not possible. 
B) There is increased risk for graft-versus-host disease. 
C) There is not a need for immunosuppression. 
D) The yield of stem cells is low.

Question 8

Which of the following occurs prior to infusion of stem cells into the recipient?

Accepted Answer

A) Blood count for ANC 
B) Chimerism studies 
C) GM-CSF infusion 
D) CD34 enumeration 
A) Blood count for ANC 
B) Chimerism studies 
C) GM-CSF infusion 
D) CD34 enumeration

Question 9

Explain the role of the laboratory professional in stem cell transplantation.

Accepted Answer

The role of the laboratory professional

Question 10

Following apheresis of a patient for an autologous stem cell transplant,the laboratician:

Accepted Answer

A) Freezes the cells in bags with DMSO 
B) Performs WBC,mononuclear cell,and CD34 cell count 
C) Purges the sample of all T8 cells 
D) Performs chimerism studies 
A) Freezes the cells in bags with DMSO 
B) Performs WBC,mononuclear cell,and CD34 cell count 
C) Purges the sample of all T8 cells 
D) Performs chimerism studies

Question 11

Which of the following determines the number of stem cells needed for a transplant procedure?

Accepted Answer

A) Patient diagnosis 
B) Patient age 
C) Patient sex 
D) Patient weight 
A) Patient diagnosis 
B) Patient age 
C) Patient sex 
D) Patient weight

Question 12

The laboratory professional is not involved in which of following steps associated with SCT?

Accepted Answer

A) Compatibility testing 
B) Pretransfusion testing 
C) Apheresis 
D) Transfusion of SCT product in patient 
A) Compatibility testing 
B) Pretransfusion testing 
C) Apheresis 
D) Transfusion of SCT product in patient

Question 13

Graft-versus-host disease must have three factors.The first is the presence of immunocompetent donor T lymphocytes.What are the remaining two factors?

Accepted Answer

A) Degree of marrow involvement and age of patient 
B) Response to previous chemotherapy and underlying disease 
C) HLA alloantigens and an immunosuppressed host 
D) Donor availability and degree of marrow involvement 
A) Degree of marrow involvement and age of patient 
B) Response to previous chemotherapy and underlying disease 
C) HLA alloantigens and an immunosuppressed host 
D) Donor availability and degree of marrow involvement

Question 14

A procedure used to decrease the potential for GVHD in an allogeneic transplant is:

Accepted Answer

A) Freeze thaw to kill lymphocytes 
B) Activation of CD4 cells and suppression of CD8 cells. 
C) Purge of CD8 lymphocytes 
D) Apheresis 
A) Freeze thaw to kill lymphocytes 
B) Activation of CD4 cells and suppression of CD8 cells. 
C) Purge of CD8 lymphocytes 
D) Apheresis

Question 15

Cord blood is a source of stem cells but must meet certain requirements before being collected.Which of the following is a factor that might lead to not collecting the cord blood?

Accepted Answer

A) More than 50 mL being available 
B) Nine-month gestation 
C) Rh negative baby 
D) Family history of inherited diseases 
A) More than 50 mL being available 
B) Nine-month gestation 
C) Rh negative baby 
D) Family history of inherited diseases

Question 16

Which of the following best represents the correct order of maturation (youngest to most mature)for hematopoiesis?

Accepted Answer

A) HSC > precursor >committed progenitor 
B) Precursor >HSC >committed progenitor 
C) HSC > committed progenitor > precursor 
D) Committed progenitor >precursor > HSC 
A) HSC > precursor >committed progenitor 
B) Precursor >HSC >committed progenitor 
C) HSC > committed progenitor > precursor 
D) Committed progenitor >precursor > HSC

Question 17

How HSCs differ from committed progenitor cells in that HSC has:

Accepted Answer

A) Ability to self-renew 
B) Success in autologous transplantation 
C) Potency in the immune response 
D) Location of maturation 
A) Ability to self-renew 
B) Success in autologous transplantation 
C) Potency in the immune response 
D) Location of maturation

Question 18

A 64-year-old patient received an SCT in March.Two weeks after the transplant,the physician ordered a routine blood count.The patient's ANC is 12 ×10⁹/L,and his platelet count is 65 × 10⁹/L.Chimerism studies were performed 6 months after his transplant using VNTR polymorphisms,which indicated a partial chimerism.Interpret these results regarding what they mean for the patient.

Accepted Answer

A) The patient achieved short-term and long-term engraftment. 
B) The patient achieved long-term engraftment but not short-term engraftment. 
C) The patient achieved short-term engraftment,but long-term results are indeterminate. 
D) The patient did not achieve engraftment at all. 
A) The patient achieved short-term and long-term engraftment. 
B) The patient achieved long-term engraftment but not short-term engraftment. 
C) The patient achieved short-term engraftment,but long-term results are indeterminate. 
D) The patient did not achieve engraftment at all.

Question 19

Which of the following agents has the ability to re-emerge in SCT patients on multiple occasions?

Accepted Answer

A) Streptococcus infection 
B) Cytomegalovirus infection 
C) Candidiasis 
D) Epstein-Barr virus infection 
A) Streptococcus infection 
B) Cytomegalovirus infection 
C) Candidiasis 
D) Epstein-Barr virus infection

Question 20

An early complication of a stem cell transplant includes:

Accepted Answer

A) Hypogonadism 
B) Development of secondary leukemia 
C) Graft-versus-host disease 
D) Neuropathies 
A) Hypogonadism 
B) Development of secondary leukemia 
C) Graft-versus-host disease 
D) Neuropathies

Exam 29: Hematopoietic Stem Cell Transplantation

Which of the following is correct regarding allogeneic stem cell transplant and ABO antigens?

Which HSC quantitation method would be optimal for verification of late-stage engraftment?

The most important antigens that need to be matched for a successful outcome in stem cell transplants are:

Explain the significance of HLA and ABO antigen compatibility in stem cell transplantation.

Graft-versus-host disease results from which of the following?

What is the single most important factor considered in donor selection for an allogeneic transplant?

One disadvantage of using autologous stem cells for treatment in a leukemia patient is:

Which of the following occurs prior to infusion of stem cells into the recipient?

Explain the role of the laboratory professional in stem cell transplantation.

Following apheresis of a patient for an autologous stem cell transplant,the laboratician:

Which of the following determines the number of stem cells needed for a transplant procedure?

The laboratory professional is not involved in which of following steps associated with SCT?

Graft-versus-host disease must have three factors.The first is the presence of immunocompetent donor T lymphocytes.What are the remaining two factors?

A procedure used to decrease the potential for GVHD in an allogeneic transplant is:

Cord blood is a source of stem cells but must meet certain requirements before being collected.Which of the following is a factor that might lead to not collecting the cord blood?

Which of the following best represents the correct order of maturation (youngest to most mature)for hematopoiesis?

How HSCs differ from committed progenitor cells in that HSC has:

Which of the following agents has the ability to re-emerge in SCT patients on multiple occasions?

An early complication of a stem cell transplant includes:

Introduction

Cellular Homeostasis

Structure and Function of Hematopoietic Organs

Hematopoiesis

The Erythrocyte

Hemoglobin

Granulocytes and Monocytes

Lymphocytes

The Platelet

The Complete Blood Count and Peripheral Blood Smear Evaluation

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopathies: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Hypoproliferative Anemias

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Disorders of Leukocytes: Granulocytes and Monocytes

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Neoplasms

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Precursor Lymphoid Neoplasms

Mature Lymphoid Neoplasms

Morphologic Analysis of Body Fluids in the Hematology Laboratory

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hemostasis: Laboratory Testing and Instrumentation

Hematology Procedures

Bone Marrow Examination

Automation in Hematology

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Molecular Analysis of Hematologic Diseases

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