Question 1

Which of the following classes of hematopoietic cells has the ability to self-renew?

Accepted Answer

A)  Hematopoietic stem cell 
B)  Multipotential progenitor cell 
C)  Committed myeloid progenitor 
D)  Maturing cell 
A)  Hematopoietic stem cell 
B)  Multipotential progenitor cell 
C)  Committed myeloid progenitor 
D)  Maturing cell A

Question 2

Which of the following cytokines inhibits stem cell proliferation?

Accepted Answer

A)  Interferon 
B)  MIP-1α 
C)  Lactoferrin 
D)  TNF-α 
A)  Interferon 
B)  MIP-1α 
C)  Lactoferrin 
D)  TNF-α B

Question 3

Which of the following best describes the phenotype of the hematopoietic stem cell?

Accepted Answer

A)  CD34+ CD38+Lin-HLA-DR-Rh123Bright, SCF-R+,TPO-R+ 
B)  CD34+ CD33+CD38+Lin- Rh123Lo 
C)  CD34+ CD38-Lin-HLA-DR-Rh123Lo, SCF-R+,TPO-R+ 
D)  CD3+,CD4+,CD5+,CD8+,CD38-,HLA-DR- 
A)  CD34+ CD38+Lin-HLA-DR-Rh123Bright, SCF-R+,TPO-R+ 
B)  CD34+ CD33+CD38+Lin- Rh123Lo 
C)  CD34+ CD38-Lin-HLA-DR-Rh123Lo, SCF-R+,TPO-R+ 
D)  CD3+,CD4+,CD5+,CD8+,CD38-,HLA-DR- C

Question 4

Which term is used to describe the point when two cells derived from the same precursor take a separate route of development?

Accepted Answer

A)  Maturation 
B)  Differentiation 
C)  Commitment 
D)  Specialization 
A)  Maturation 
B)  Differentiation 
C)  Commitment 
D)  Specialization

Question 5

Which of the following types of cell signaling describes how SCF and Flt3 ligand predominately regulate stem cell homeostasis?

Accepted Answer

A)  Autocrine 
B)  Paracrine 
C)  Juxtacrine 
D)  Endocrine 
A)  Autocrine 
B)  Paracrine 
C)  Juxtacrine 
D)  Endocrine

Question 6

Which of the following molecules (synthetically generated) is administered to patients with renal disease to stimulate red cell production?

Accepted Answer

A)  GM-CSF 
B)  Interleukin-2 
C)  EPO 
D)  G-CSF 
A)  GM-CSF 
B)  Interleukin-2 
C)  EPO 
D)  G-CSF

Question 7

All of the following are negative regulators of hematopoiesis except:

Accepted Answer

A)  TGF-β 
B)  TNF 
C)  Kit ligand 
D)  MIP-1α 
A)  TGF-β 
B)  TNF 
C)  Kit ligand 
D)  MIP-1α

Question 8

Which of the following statements describes the role of transcription factors (TF) in hematopoiesis?

Accepted Answer

A)  TF expression does not change during hematopoiesis. 
B)  TF function either to activate or suppress target gene expression. 
C)  TF block the differentiation pathway for hematopoietic precursors. 
D)  TF abnormalities are rarely seen in hematologic malignancies. 
A)  TF expression does not change during hematopoiesis. 
B)  TF function either to activate or suppress target gene expression. 
C)  TF block the differentiation pathway for hematopoietic precursors. 
D)  TF abnormalities are rarely seen in hematologic malignancies.

Question 9

Which components are included in the stromal cell compartment?

Accepted Answer

A)  Matrix proteins and regulatory cytokines 
B)  Adipocytes, endothelial cells, fibroblasts, T lymphocytes, and macrophages 
C)  Stem cells 
D)  Progenitor cells 
A)  Matrix proteins and regulatory cytokines 
B)  Adipocytes, endothelial cells, fibroblasts, T lymphocytes, and macrophages 
C)  Stem cells 
D)  Progenitor cells

Question 10

Signal transduction pathways are propagated primarily through which of the following?

Accepted Answer

A)  Kinases and protein phosphorylation 
B)  Phosphatases and protein dephosphorylation 
C)  Protein methylation 
D)  Histone deacetylation 
A)  Kinases and protein phosphorylation 
B)  Phosphatases and protein dephosphorylation 
C)  Protein methylation 
D)  Histone deacetylation

Question 11

Stem cells and progenitor cells can be measured by clonal assays in vitro. Maturing cells are measured by:

Accepted Answer

A)  Clonal assays 
B)  Phenotyping 
C)  Flow cytometry 
D)  Morphologic analysis 
A)  Clonal assays 
B)  Phenotyping 
C)  Flow cytometry 
D)  Morphologic analysis

Question 12

Which of the following classes of hematopoietic cells is committed to becoming an erythrocyte?

Accepted Answer

A)  Hematopoietic stem cell 
B)  Multipotential progenitor cell 
C)  Committed lymphoid progenitor 
D)  BFU-E 
A)  Hematopoietic stem cell 
B)  Multipotential progenitor cell 
C)  Committed lymphoid progenitor 
D)  BFU-E

Question 13

Explain how hematopoiesis uses autocrine, paracrine, and juxtacrine signaling. Choose one and provide an example of signaling through that pathway.

Accepted Answer

Autocrine signaling: produced by and act

Question 14

Which of the following lineages are derived from the bipotential progenitor cell MEP?

Accepted Answer

A)  Erythrocytes and megakaryocytes 
B)  Eosinophils and megakaryocytes 
C)  Erythrocytes and monocytes 
D)  Eosinophils and monocytes 
A)  Erythrocytes and megakaryocytes 
B)  Eosinophils and megakaryocytes 
C)  Erythrocytes and monocytes 
D)  Eosinophils and monocytes

Question 15

Explain the JAK-STAT signaling pathway, and describe how it can contribute to disease.

Accepted Answer

JAK: Janus family PTKs (protein tyrosine

Question 16

GM-CSF is considered a(n):

Accepted Answer

A)  Early-acting cytokine 
B)  Late-acting cytokine 
C)  Lineage-specific cytokine 
D)  Early-acting and multilineage cytokine 
A)  Early-acting cytokine 
B)  Late-acting cytokine 
C)  Lineage-specific cytokine 
D)  Early-acting and multilineage cytokine

Question 17

Which lineage promotes differentiation and maturation primarily with the use of interleukins?

Accepted Answer

A)  Monocytes 
B)  Erythrocytes 
C)  Platelets 
D)  Lymphocytes 
A)  Monocytes 
B)  Erythrocytes 
C)  Platelets 
D)  Lymphocytes

Question 18

Explain the role of selected cytokines in the treatment of disorders affecting the hematopoietic system:
a. EPO
b. CSF3/CSF2
c. IL-3/CSF2/EPO

Accepted Answer

a. EPO: stimulation of erythro

Question 19

Cytokine receptors that lack an intrinsic kinase domain transmit signals primarily through:

Accepted Answer

A)  Transmembrane protein activation 
B)  Activation of cytoplasmic kinases 
C)  Heterodimer integral protein activation 
D)  Activation of mitochondrial networks 
A)  Transmembrane protein activation 
B)  Activation of cytoplasmic kinases 
C)  Heterodimer integral protein activation 
D)  Activation of mitochondrial networks

Question 20

Which component allows the hematopoietic precursors to attach to the bone marrow microenvironment?

Accepted Answer

A)  L-6 
B)  TNF 
C)  CAM 
D)  IFN 
A)  L-6 
B)  TNF 
C)  CAM 
D)  IFN

Exam 4: Hematopoiesis

Which of the following classes of hematopoietic cells has the ability to self-renew?

Which of the following cytokines inhibits stem cell proliferation?

Which of the following best describes the phenotype of the hematopoietic stem cell?

Which term is used to describe the point when two cells derived from the same precursor take a separate route of development?

Which of the following types of cell signaling describes how SCF and Flt3 ligand predominately regulate stem cell homeostasis?

Which of the following molecules (synthetically generated) is administered to patients with renal disease to stimulate red cell production?

All of the following are negative regulators of hematopoiesis except:

Which of the following statements describes the role of transcription factors (TF) in hematopoiesis?

Which components are included in the stromal cell compartment?

Signal transduction pathways are propagated primarily through which of the following?

Stem cells and progenitor cells can be measured by clonal assays in vitro. Maturing cells are measured by:

Which of the following classes of hematopoietic cells is committed to becoming an erythrocyte?

Explain how hematopoiesis uses autocrine, paracrine, and juxtacrine signaling. Choose one and provide an example of signaling through that pathway.

Which of the following lineages are derived from the bipotential progenitor cell MEP?

Explain the JAK-STAT signaling pathway, and describe how it can contribute to disease.

GM-CSF is considered a(n):

Which lineage promotes differentiation and maturation primarily with the use of interleukins?

Explain the role of selected cytokines in the treatment of disorders affecting the hematopoietic system: a. EPO b. CSF3/CSF2 c. IL-3/CSF2/EPO

Cytokine receptors that lack an intrinsic kinase domain transmit signals primarily through:

Which component allows the hematopoietic precursors to attach to the bone marrow microenvironment?

Introduction

Cellular Homeostasis

Structure and Function of Hematopoietic Organs

The Erythrocyte

Hemoglobin

Granulocytes and Monocytes

Lymphocytes

The Platelet

The Complete Blood Count and Peripheral Blood Smear Evaluation

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopathies: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Hypoproliferative Anemias

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Disorders of Leukocytes: Granulocytes and Monocytes

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Neoplasms

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Precursor Lymphoid Neoplasms

Mature Lymphoid Neoplasms

Hematopoietic Stem Cell Transplantation

Morphologic Analysis of Body Fluids in the Hematology

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hemostasis: Laboratory Testing and Instrumentation

Hematology Procedures

Bone Marrow Examination

Automation in Hematology

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Molecular Analysis of Hematologic Diseases

Quality Assessment in the Hematology Laboratory

Filters