Exam 40: Flow Cytometry

Neoplasms are made up of a population of identical cells or a clone of cells. When identified, the clone can lead to the discovery of a lymphoid malignancy or other lymphoproliferative disorder. Clonality be detected by:

Which of the following is considered a drawback of immunophenotyping a suspected acute leukemia?

CD34 enumeration is useful in bone marrow and peripheral blood stem cell transplantation. For this enumeration, the EWGCCA recommendations include proper technique and interpretation of the flow cytometry analysis. Which of the following standards was recommended for proper acquisition of events?

The EWGCCA recommends performing which of the following with CD34 enumeration?

How is flow cytometry used in cell quantitation?

A flow instrument printout reveals a single scatter population of small size and low complexity. Ninety percent of the cells in that population have CD2 on their surface. What is the most probable lineage of the unknown population?

An unknown cell resembling a hematogone has CD19 and CD20 antigens. What is the unknown cell's lineage?

Which analyte is the best indicator of the overall picture of the immune system in an HIV-positive patient?

Flow results on a patient sample revealed an absence of CD20 and CD19 on the B-lymphocytes as well as an absence of BTK in monocytes and platelets. What is the most likely diagnosis?

Which markers are useful in immunophenotyping for leukemia/lymphoma of T cell-lineage?

Immunophenotyping is essential for diagnosing ALL, separating T- and B- lineage ALL, and identifying subtypes of B lineage ALL. What markers can blasts express in precursor B cell ALL?

Name at least two pitfalls in immunophenotyping mature lymphoid malignancies, and explain how you would correct this.

Rituximab, an anti-CD20 antibody, has been found to be effective in treating:

The DNA content of a patient's tumor cells is reported as 2.0, and the DNA content of diploid cells is 1.0. What is the DNA index?

The following are accurate statements regarding fluorochromes except which one?

What is one advantage to using monoclonal antibodies rather than polyclonal antibodies in immunophenotyping?

Which of the following is not the component(s) of a flow cytometer?

A strategy for determining MRD in AML that has been advocated is:

The expression of several antigens from two different lineages complicates the categorizing the diagnosis as AML or ALL. This biphenotypic expression toward more than one lineage restricts the use of immunophenotyping. In differentiating AML and ALL, staining with which of the following cytochemical stains indicates AML?

What is the purpose of CD34 count?