Exam 37: Hematology Procedures

How does the anticoagulant EDTA prevent coagulation from occurring?

The reference interval for hemoglobin varies with age. Which of the following age groups is associated with the highest hemoglobin level?

What is the anticoagulant in the most commonly utilized tube in hematology, and how does it prevent coagulation from taking place?

In the chloroacetate esterase procedure, which reagent component undergoes color change because of the enzyme's activity?

List corrective measurements for each of the following: a. A good monolayer on a blood smear in a patient with a low hematocrit b. A good monolayer on a blood smear in a patient with a high hematocrit c. Platelet satellitism d. Many smudge cells on PB smear

In phase contrast microscopy, the function of the phase ring is to:

A patient has difficult veins, and a winged infusion set is used to collect the blood from a small vein in the patient's hand. After successfully filling all the SST tubes, the phlebotomist proceeds to fill the light blue-top tube but is unsuccessful. She changes tubes, thinking that it might be a tube with a lost vacuum, but there is still no blood flow. She inspects the entry site and notices some bruising around it that was not present before. Discuss possibilities of the unsuccessful draw, and explain how to correct them.

The following erythrocyte data were obtained from an EDTA-anticoagulated sample: erythrocyte count = 3.92 × 10¹²/L, hemoglobin = 11.4 g/dL, and hematocrit = 34% (.34 L/L). Calculate the MCHC.

The following erythrocyte indices were determined as part of a CBC: MCV 84 fL, MCH 24 pg, and MCHC 30 g/dL. What would you expect to observe on the Wright-stained peripheral blood smear?

Which cytochemical stain is useful in differentiating CML from a leukemoid reaction?

Which of the following receptacles is most appropriate when disposing of used needles?

Which of the following is the most appropriate test to differentiate hemoglobin S trait from hemoglobin S disease?

Which cytochemical stain is useful in differentiating myeloblasts and lymphoblasts?

The ESR determination on the MINI-VES instrument is based on:

Two incubation sessions are needed in the Donath-Landsteiner test to detect:

Increased levels of Heinz bodies are seen in which of the following?

The following are preliminary screening test results. Name the suspected disorder associated with each finding, and explain how you would confirm each. a. Positive hemolysis after both incubation periods b. Normal sTFR levels c. Many spherocytes noted on PB smear d. Blasts noted on PB smear

The microscopic examination of a Wright-stained blood smear revealed bright red erythrocytes and pale leukocyte nuclei. What is the best explanation for this appearance?

A patient is about to begin a regimen of chemotherapy, and his attending physician has ordered a battery of lab tests including comprehensive chemistry metabolic panel, complete blood count, coagulation studies, amylase and lipase, hemoglobin A1C, lipid panel, thyroid panel, PSA, rubella IgG, HIV, and syphilis. Five tiger-top tubes, 2 light blue-top tubes, and 2 lavender-top tubes are needed. Correlate each test to the appropriate tube and indicate the correct order of draw for the tubes.

The 18-gauge needles used to collect donor blood for transfusion have a larger bore than do standard blood collection needles. What is the advantage of this needle?