Question 1

A Hodgkin lymphoma patient is admitted for treatment. What would be the benefit in performing an EBV-DNA test on him?

Accepted Answer

A)  It would identify the cause of the Hodgkin lymphoma. 
B)  Because the patient is immunocompromised, it would identify an opportunistic pathogen. 
C)  It would help monitor the efficacy of his treatment. 
D)  It would help rule out other types of lymphomas. 
A)  It would identify the cause of the Hodgkin lymphoma. 
B)  Because the patient is immunocompromised, it would identify an opportunistic pathogen. 
C)  It would help monitor the efficacy of his treatment. 
D)  It would help rule out other types of lymphomas. C

Question 2

What is the definition of allele?

Accepted Answer

A)  A fundamental substance of heredity that cells use to catalog, express, and propagate information 
B)  The two versions of a gene that are inherited from each parent 
C)  A messenger molecule that translates the DNA code to induce protein production 
D)  The functional unit composed of amino acids 
A)  A fundamental substance of heredity that cells use to catalog, express, and propagate information 
B)  The two versions of a gene that are inherited from each parent 
C)  A messenger molecule that translates the DNA code to induce protein production 
D)  The functional unit composed of amino acids B

Question 3

Which molecular technique is useful in monitoring the amount of pathogen-specific nucleic acids in patients with an infectious disease?

Accepted Answer

A)  ASO 
B)  FISH 
C)  DNA amplification and sequencing 
D)  qPCR 
A)  ASO 
B)  FISH 
C)  DNA amplification and sequencing 
D)  qPCR D

Question 4

Explain the central doctrine of molecular biology using proper nomenclature. Define each phase.

Accepted Answer

The central doctrine of molecular biolog

Question 5

A 23-year-old female is admitted to the local hospital with a deep vein thrombosis. In addition to initial laboratory workup, which of the following should be investigated as possible causes of the DVT?

Accepted Answer

A)  PCR for sickle cell mutation 
B)  FV and prothrombin mutations 
C)  BCR/ABL1 fusion gene mutation 
D)  TCR gene mutations 
A)  PCR for sickle cell mutation 
B)  FV and prothrombin mutations 
C)  BCR/ABL1 fusion gene mutation 
D)  TCR gene mutations

Question 6

Explain why molecular testing is essential not only in identifying disorders, such as cancer, but also during the course of a disease.

Accepted Answer

Unlike inherited disease, in which every

Question 7

The use of a labeled probe to detect and localize specific RNA or DNA sequences in tissue samples describes which of the following techniques:

Accepted Answer

A)  ASO 
B)  PCR 
C)  qPCR 
D)  FISH 
A)  ASO 
B)  PCR 
C)  qPCR 
D)  FISH

Question 8

Name and compare two molecular methods used to detect mutations associated with sickle cell disease.

Accepted Answer

Two molecular methods that detect the mu

Question 9

Which of the following is associated with chronic myelogenous leukemia?

Accepted Answer

A)  BCR/ABL1 fusion gene 
B)  t(15;17) 
C)  t(8,14) 
D)  PML/RARA fusion gene 
A)  BCR/ABL1 fusion gene 
B)  t(15;17) 
C)  t(8,14) 
D)  PML/RARA fusion gene

Question 10

Explain the impact of a positive test for HTLV-1 in a lymphoma patient.

Accepted Answer

HTLV1 infects T-helper lymphocytes and c

Question 11

Minimal residual disease testing is a molecular application for which hematopathology?

Accepted Answer

A)  Leukemia/lymphoma 
B)  Sickle cell disease 
C)  Cancer 
D)  Gene mutations 
A)  Leukemia/lymphoma 
B)  Sickle cell disease 
C)  Cancer 
D)  Gene mutations

Question 12

A multiplex reaction that targets conserved regions within the variable (V), diversity (D), and joining (J) regions of the immunoglobulin heavy chain can be used to determine clonality in which neoplasm?

Accepted Answer

A)  CML 
B)  AML 
C)  B-cell leukemia/lymphoma 
D)  Human T-lymphocytic virus type 1 
A)  CML 
B)  AML 
C)  B-cell leukemia/lymphoma 
D)  Human T-lymphocytic virus type 1

Question 13

Give the advantages of PCR over Southern blot and advantages of Southern blot over PCR.

Accepted Answer

Advantages of PCR over Southern blot: sm

Question 14

Which of the following methods would be most useful for identifying point mutations anywhere in the DNA segment?

Accepted Answer

A)  Southern blot analysis 
B)  PCR 
C)  DNA sequencing 
D)  FISH 
A)  Southern blot analysis 
B)  PCR 
C)  DNA sequencing 
D)  FISH

Question 15

Which of the following assays would be most appropriate for monitoring minimal residual disease?

Accepted Answer

A)  Karyotyping 
B)  Southern blot analysis 
C)  qPCR 
A)  Karyotyping 
B)  Southern blot analysis 
C)  qPCR

Question 16

In reference to detecting minimal residual disease, which of the following is true?

Accepted Answer

A)  PCR, karyotyping, and Southern blot analysis are equally sensitive and specific. 
B)  qPCR is most sensitive for detecting low levels of tumor burden. 
C)  Karyotyping is capable of detecting a 0.1% tumor burden. 
D)  Southern blot analysis has 100% sensitivity and specificity for detecting aberrant proteins formed as a result of the genetic mutation. 
A)  PCR, karyotyping, and Southern blot analysis are equally sensitive and specific. 
B)  qPCR is most sensitive for detecting low levels of tumor burden. 
C)  Karyotyping is capable of detecting a 0.1% tumor burden. 
D)  Southern blot analysis has 100% sensitivity and specificity for detecting aberrant proteins formed as a result of the genetic mutation.

Question 17

Which of the following molecular techniques is useful in detecting the point mutations in a suspected hemoglobinopathy:

Accepted Answer

A)  DNA amplification and sequencing 
B)  qPCR 
C)  High-resolution melt curve analysis 
D)  RT-PCR 
A)  DNA amplification and sequencing 
B)  qPCR 
C)  High-resolution melt curve analysis 
D)  RT-PCR

Exam 42: Molecular Analysis of Hematologic Diseases

A Hodgkin lymphoma patient is admitted for treatment. What would be the benefit in performing an EBV-DNA test on him?

What is the definition of allele?

Which molecular technique is useful in monitoring the amount of pathogen-specific nucleic acids in patients with an infectious disease?

Explain the central doctrine of molecular biology using proper nomenclature. Define each phase.

A 23-year-old female is admitted to the local hospital with a deep vein thrombosis. In addition to initial laboratory workup, which of the following should be investigated as possible causes of the DVT?

Explain why molecular testing is essential not only in identifying disorders, such as cancer, but also during the course of a disease.

The use of a labeled probe to detect and localize specific RNA or DNA sequences in tissue samples describes which of the following techniques:

Name and compare two molecular methods used to detect mutations associated with sickle cell disease.

Which of the following is associated with chronic myelogenous leukemia?

Explain the impact of a positive test for HTLV-1 in a lymphoma patient.

Minimal residual disease testing is a molecular application for which hematopathology?

A multiplex reaction that targets conserved regions within the variable (V), diversity (D), and joining (J) regions of the immunoglobulin heavy chain can be used to determine clonality in which neoplasm?

Give the advantages of PCR over Southern blot and advantages of Southern blot over PCR.

Which of the following methods would be most useful for identifying point mutations anywhere in the DNA segment?

Which of the following assays would be most appropriate for monitoring minimal residual disease?

In reference to detecting minimal residual disease, which of the following is true?

Which of the following molecular techniques is useful in detecting the point mutations in a suspected hemoglobinopathy:

Introduction

Cellular Homeostasis

Structure and Function of Hematopoietic Organs

Hematopoiesis

The Erythrocyte

Hemoglobin

Granulocytes and Monocytes

Lymphocytes

The Platelet

The Complete Blood Count and Peripheral Blood Smear Evaluation

Introduction to Anemia

Anemias of Disordered Iron Metabolism and Heme Synthesis

Hemoglobinopathies: Qualitative Defects

Thalassemia

Megaloblastic and Nonmegaloblastic Macrocytic Anemias

Hypoproliferative Anemias

Hemolytic Anemia: Membrane Defects

Hemolytic Anemia: Enzyme Deficiencies

Hemolytic Anemia: Immune Anemias

Hemolytic Anemia: Nonimmune Defects

Nonmalignant Disorders of Leukocytes: Granulocytes and Monocytes

Nonmalignant Lymphocyte Disorders

Introduction to Hematopoietic Neoplasms

Myeloproliferative Neoplasms

Myelodysplastic Syndromes

Acute Myeloid Leukemias

Precursor Lymphoid Neoplasms

Mature Lymphoid Neoplasms

Hematopoietic Stem Cell Transplantation

Morphologic Analysis of Body Fluids in the Hematology

Primary Hemostasis

Secondary Hemostasis and Fibrinolysis

Disorders of Primary Hemostasis

Disorders of Secondary Hemostasis

Thrombophilia

Hemostasis: Laboratory Testing and Instrumentation

Hematology Procedures

Bone Marrow Examination

Automation in Hematology

Flow Cytometry

Chromosome Analysis of Hematopoietic and Lymphoid Disorders

Quality Assessment in the Hematology Laboratory

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