Exam 40: Flow Cytometry

The flow cytometer contains a flow chamber that has two columns of fluid-sample and sheath-maintained at different pressure. Which of the following statements about the flow chamber is correct?

The fluorescent dye employed by hematology analyzers to count reticulocytes binds to:

Cells can be separated during data analysis by gating. This process of isolating cells can be based on what properties?

In diagnosing PNH, which of the following assays is most sensitive to its detection?

Which two CD markers are helpful in differentially identifying CLL from hairy cell leukemia?

Of the following, which is not an appropriate specimen for flow cytometry?

Which of the following is(are) the clinical application(s) of flow cytometry?

The percentage of CD4 positive lymphocytes is determined by flow cytometry using staining with fluorescent-tagged antibodies against CD3 and CD4 followed by whole blood RBC lysis. What calculations does the dual platform use to determine the absolute number of CD4 positive cells?

The gold standard for the diagnosis of AML is based on what information?

An HIV-positive patient has his blood drawn to get his CD4 count. His results are as follows: WBC count: 2.0 × 10⁹/L with 15% lymphocytes. CD4 positive cells are 10% of the total lymphocyte count. What is this patient's absolute CD4 count, and what does this mean?

A single group of cells possessing identical aberrant phenotypes is defined as:

A blood sample from a patient suspected of having ALL is received in the flow lab for immunophenotyping. The analysis is performed, and standard B-cell markers are present, but the patient is also CD33-positive. What is the most likely reason for this occurrence?

Which of the following is used to determine the relationship between the content of tumor cells and normal dividing cells?