Question 1

To avoid the introduction of introns into the vector, a ____ copy of mature mRNA is made, using the enzyme ____.

Accepted Answer

A)  sDNA; RNA polymerase 
B)  cDNA; DNA ligase 
C)  cDNA; reverse transcriptase 
D)  sDNA; reverse transcriptase 
E)  cDNA; DNA polymerase 
A)  sDNA; RNA polymerase 
B)  cDNA; DNA ligase 
C)  cDNA; reverse transcriptase 
D)  sDNA; reverse transcriptase 
E)  cDNA; DNA polymerase

Question 2

Bacteriophages are:

Accepted Answer

A)  viruses that infect bacteria. 
B)  plasmids that infect bacteria. 
C)  bacteria that infect other bacteria. 
D)  enzymes that destroy bacteria. 
E)  recombinant organisms. 
A)  viruses that infect bacteria. 
B)  plasmids that infect bacteria. 
C)  bacteria that infect other bacteria. 
D)  enzymes that destroy bacteria. 
E)  recombinant organisms.

Question 3

cDNA libraries contain:

Accepted Answer

A)  single stranded DNA. 
B)  DNA synthesized with the use of DNA polymerase. 
C)  DNA synthesized from and complementary to an mRNA template. 
D)  introns and exons. 
E)  genomic DNA 
A)  single stranded DNA. 
B)  DNA synthesized with the use of DNA polymerase. 
C)  DNA synthesized from and complementary to an mRNA template. 
D)  introns and exons. 
E)  genomic DNA

Question 4

Explain how herbicide-tolerant weeds ("superweeds") could appear in areas nearby transgenic herbicide-tolerant crops. What issues arise with this scenario?

Accepted Answer

Transgenic herbicide-tolerant crops may

Question 5

Transgenic salmon are genetically engineered to grow faster than their wild type counterparts. They do not grow larger; they simply reach their full size in shorter time. Transgenic salmon that are grown in outdoor pens are all sterile (non-reproducing) females. Why is this important?

Accepted Answer

If the transgenic fish escape from their

Question 6

Figure 15-1   Refer to the accompanying figure. The use of antibiotic medium at the end of this process:

Accepted Answer

A)  selects against plasmids containing human DNA fragments. 
B)  selects for plasmids containing particular DNA fragments. 
C)  selects for bacteria containing plasmids. 
D)  selects for bacteria lacking plasmids. 
E)  prevents contamination of the medium. 
A)  selects against plasmids containing human DNA fragments. 
B)  selects for plasmids containing particular DNA fragments. 
C)  selects for bacteria containing plasmids. 
D)  selects for bacteria lacking plasmids. 
E)  prevents contamination of the medium.

Question 7

Bacteria methylate their DNA in order to:

Accepted Answer

A)  prepare for DNA replication. 
B)  prepare for mitosis. 
C)  effectively infect other cells. 
D)  protect it from restriction enzymes. 
E)  prevent viral DNA from hybridizing with bacterial DNA. 
A)  prepare for DNA replication. 
B)  prepare for mitosis. 
C)  effectively infect other cells. 
D)  protect it from restriction enzymes. 
E)  prevent viral DNA from hybridizing with bacterial DNA.

Question 8

Retroviruses make ____ by the process of ____.

Accepted Answer

A)  RNA copies of DNA; reverse transcription 
B)  DNA copies of RNA; reverse transcription 
C)  genetic probes; Southern blotting 
D)  genetic probes; Northern blotting 
E)  telomerase; mutagenesis 
A)  RNA copies of DNA; reverse transcription 
B)  DNA copies of RNA; reverse transcription 
C)  genetic probes; Southern blotting 
D)  genetic probes; Northern blotting 
E)  telomerase; mutagenesis

Question 9

When RNAs separated by electrophoresis are transferred to a membrane and detected using a nucleic acid probe, the result is called a(n) Western Blot . __________________

Accepted Answer

A) True 
 B)False

Question 10

The polymerase chain reaction was developed by:

Accepted Answer

A)  Francis Crick. 
B)  Walter Gilbert. 
C)  E.M. Southern. 
D)  Kary Mullis. 
E)  Leland Hartwell. 
A)  Francis Crick. 
B)  Walter Gilbert. 
C)  E.M. Southern. 
D)  Kary Mullis. 
E)  Leland Hartwell.

Question 11

Figure 15-1   What process or enzyme was used for Step 1 in the accompanying figure?

Accepted Answer

A)  PCR 
B)  a bacteriophage 
C)  a reverse transcriptase 
D)  a DNA ligase 
E)  a restriction enzyme 
A)  PCR 
B)  a bacteriophage 
C)  a reverse transcriptase 
D)  a DNA ligase 
E)  a restriction enzyme

Question 12

The amplification of recombinant plasmids occurs by:

Accepted Answer

A)  the growth of the plasmid DNA. 
B)  DNA replication of plasmids outside a host cell. 
C)  the process of growth and division of the host cell. 
D)  transformation of bacterial cells. 
E)  the polymerase chain reaction. 
A)  the growth of the plasmid DNA. 
B)  DNA replication of plasmids outside a host cell. 
C)  the process of growth and division of the host cell. 
D)  transformation of bacterial cells. 
E)  the polymerase chain reaction.

Question 13

Why is it unlikely that genetically engineered strains of bacteria would pose a threat to the environment?

Accepted Answer

A)  Genetically engineered bacteria cannot survive exposure to UV radiation. 
B)  Genetically engineered strains of bacteria are strictly regulated, making accidental release unlikely. 
C)  Genetically engineered strains of bacteria cannot survive exposure to oxygen. 
D)  Genetically engineered strains of bacteria are poor competitors of wild strains. 
E)  Genetically engineered strains of bacteria require supplemental nutrients to survive. 
A)  Genetically engineered bacteria cannot survive exposure to UV radiation. 
B)  Genetically engineered strains of bacteria are strictly regulated, making accidental release unlikely. 
C)  Genetically engineered strains of bacteria cannot survive exposure to oxygen. 
D)  Genetically engineered strains of bacteria are poor competitors of wild strains. 
E)  Genetically engineered strains of bacteria require supplemental nutrients to survive.

Question 14

Match between columns
                        Premises: Step 4
Step 1
Step 2
Step 3
Step 5

Accepted Answer

The Answer of Load the DNA fragments on the gel is...

Question 15

The total DNA in a cell is referred to as the:

Accepted Answer

A)  genome. 
B)  cDNA. 
C)  library. 
D)  chromosomal DNA. 
E)  Human Genome project 
A)  genome. 
B)  cDNA. 
C)  library. 
D)  chromosomal DNA. 
E)  Human Genome project

Question 16

Clues to the structure and function of a gene can be gained through bioinformatics .

Accepted Answer

A) True 
 B)False

Question 17

Southern blotting is a process where ____ is transferred from a gel to a ____where a probe can be applied.

Accepted Answer

A)  DNA; water solution 
B)  protein; water solution 
C)  DNA; nitrocellulose filter 
D)  RNA; nitrocellulose filter 
E)  protein; nitrocellulose filter 
A)  DNA; water solution 
B)  protein; water solution 
C)  DNA; nitrocellulose filter 
D)  RNA; nitrocellulose filter 
E)  protein; nitrocellulose filter

Question 18

____ is a technique that can be used to separate DNA molecules on the basis of their size.

Accepted Answer

A)  Separation gradient 
B)  Electrophoresis 
C)  Selective sorting 
D)  Cloning 
E)  PCR 
A)  Separation gradient 
B)  Electrophoresis 
C)  Selective sorting 
D)  Cloning 
E)  PCR

Question 19

Match between columns
                        Premises: Transgenic pest-resistant crops may kill all vulnerable individuals, creating a population of pesticide-resistant pests.
Transgenic lab animals, such as mice, are engineered to carry human genes, allowing for better study of human diseases.
Farmers who plant transgenic herbicide-tolerant crops tend to use excess herbicides.
Transgenic pest-resistant crops may affect non-target pests.
Transgenic papayas resistant to the ring spot virus saved the papaya industry in Hawai'i.
Growing transgenic pest-resistant crops means less pesticide spraying.
All transgenic salmon are sterile females; therefore, they cannot breed with wild salmon populations.
Gene flow from transgenic herbicide-tolerant plants may create herbicide-tolerant weeds.
Transgenic salmon that grow faster than wild salmon may result in less pollution from salmon farms.
Transgenic animals, such as sheep and cows, produce medicinal proteins in their milk. This creates a larger and cheaper supply of some human drugs.

Accepted Answer

The Answer of Advantage and Disadvantage is...

Question 20

List and briefly explain three properties of plasmids that allow them to be used as effective DNA cloning vectors.

Accepted Answer

Plasmids have an origin of rep

Exam 15: Dna Technology and Genomics

To avoid the introduction of introns into the vector, a ____ copy of mature mRNA is made, using the enzyme ____.

Bacteriophages are:

cDNA libraries contain:

Explain how herbicide-tolerant weeds ("superweeds") could appear in areas nearby transgenic herbicide-tolerant crops. What issues arise with this scenario?

Transgenic salmon are genetically engineered to grow faster than their wild type counterparts. They do not grow larger; they simply reach their full size in shorter time. Transgenic salmon that are grown in outdoor pens are all sterile (non-reproducing) females. Why is this important?

Figure 15-1 Refer to the accompanying figure. The use of antibiotic medium at the end of this process:

Bacteria methylate their DNA in order to:

Retroviruses make ____ by the process of ____.

When RNAs separated by electrophoresis are transferred to a membrane and detected using a nucleic acid probe, the result is called a(n) Western Blot . __________________

The polymerase chain reaction was developed by:

Figure 15-1 What process or enzyme was used for Step 1 in the accompanying figure?

The amplification of recombinant plasmids occurs by:

Why is it unlikely that genetically engineered strains of bacteria would pose a threat to the environment?

The total DNA in a cell is referred to as the:

Clues to the structure and function of a gene can be gained through bioinformatics .

Southern blotting is a process where ____ is transferred from a gel to a ____where a probe can be applied.

____ is a technique that can be used to separate DNA molecules on the basis of their size.

List and briefly explain three properties of plasmids that allow them to be used as effective DNA cloning vectors.

A View of Life

Atoms and Molecules the Chemical Basis of Life

The Chemistry of Life Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp Energy-Releasing Pathways

Photosynthesis Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

Dna the Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure Growth and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function an Introduction

Protection Support and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

Filters

To avoid the introduction of introns into the vector, a copy of mature mRNA is made, using the enzyme .

Retroviruses make by the process of .

Southern blotting is a process where is transferred from a gel to a where a probe can be applied.