Question 1

If a protein-coding gene is identified, its function can be studied by using RNA interference to ____.

Accepted Answer

A)  produce an RFLP 
B)  determine the DNA sequence. 
C)  turn the gene on 
D)  turn the gene off 
E)  induce a mutation 
A)  produce an RFLP 
B)  determine the DNA sequence. 
C)  turn the gene on 
D)  turn the gene off 
E)  induce a mutation D

Question 2

An organism in which foreign genes have been incorporated is called a:

Accepted Answer

A)  recombinant organism. 
B)  transgene. 
C)  polymorphism 
D)  transgenic organism. 
E)  recombinant. 
A)  recombinant organism. 
B)  transgene. 
C)  polymorphism 
D)  transgenic organism. 
E)  recombinant. D

Question 3

The chain termination method of DNA sequencing is based on a replicating DNA strand incorporating a(n) deoxynucleotide that stops the chain elongation. 
__________________

Accepted Answer

A) True 
 B)False  False

Question 4

A genomic library is a collection of:

Accepted Answer

A)  plasmid fragments that are spliced into genomic DNA. 
B)  genomic DNA fragments that are spliced into plasmids. 
C)  intron fragments that are spliced into plasmids. 
D)  exon fragments that are spliced into bacterial cells. 
E)  gene fragments that are spliced into main bacterial chromosome. 
A)  plasmid fragments that are spliced into genomic DNA. 
B)  genomic DNA fragments that are spliced into plasmids. 
C)  intron fragments that are spliced into plasmids. 
D)  exon fragments that are spliced into bacterial cells. 
E)  gene fragments that are spliced into main bacterial chromosome.

Question 5

When DNA ligase is used to combine a human fragment with a plasmid, the result is a(n) redundant plasmid. 
__________________

Accepted Answer

A) True 
 B)False

Question 6

Splicing together DNA from two different organisms is known as:

Accepted Answer

A)  bioengineering. 
B)  in vitro gene technology. 
C)  genome technology. 
D)  recombinant DNA technology. 
E)  DNA sequencing. 
A)  bioengineering. 
B)  in vitro gene technology. 
C)  genome technology. 
D)  recombinant DNA technology. 
E)  DNA sequencing.

Question 7

What is one feature of "engineered" plasmids that is helpful in the isolation and analysis of cloned DNA?

Accepted Answer

A)  They can only handle DNA fragments of up to 10 kb. 
B)  They are an integral part of all eukaryotic cells. 
C)  They contain no genetic material of their own so that the cloned fragment is truly isolated. 
D)  The presence of genes that allow transformed cells to survive on different media. 
E)  The base sequence of one strand reads the same as its complement. 
A)  They can only handle DNA fragments of up to 10 kb. 
B)  They are an integral part of all eukaryotic cells. 
C)  They contain no genetic material of their own so that the cloned fragment is truly isolated. 
D)  The presence of genes that allow transformed cells to survive on different media. 
E)  The base sequence of one strand reads the same as its complement.

Question 8

What purpose do restriction enzymes play in bacterial cells?

Accepted Answer

A)  Restriction enzymes prevent the overproduction of mRNA in the bacterial cell. 
B)  Restriction enzymes attack bacteriophage DNA when it enters the cell. 
C)  Restriction enzymes promote bonding of the RNA polymerase to the promoter. 
D)  Restriction enzymes limit the rate of bacterial replication. 
E)  Restriction enzymes connect Okazaki fragments. 
A)  Restriction enzymes prevent the overproduction of mRNA in the bacterial cell. 
B)  Restriction enzymes attack bacteriophage DNA when it enters the cell. 
C)  Restriction enzymes promote bonding of the RNA polymerase to the promoter. 
D)  Restriction enzymes limit the rate of bacterial replication. 
E)  Restriction enzymes connect Okazaki fragments.

Question 9

Scientists study animal models in order to reveal the functions of human genes. This is an example of:

Accepted Answer

A)  comparative genomics. 
B)  DNA cloning. 
C)  Western blotting. 
D)  RNAi 
E)  gene targeting. 
A)  comparative genomics. 
B)  DNA cloning. 
C)  Western blotting. 
D)  RNAi 
E)  gene targeting.

Question 10

The FBI currently determines an individual's DNA profile using:

Accepted Answer

A)  DNA sequencing. 
B)  mitochondrial DNA variations. 
C)  RFLPs. 
D)  DNA ligation. 
E)  13 STR markers. 
A)  DNA sequencing. 
B)  mitochondrial DNA variations. 
C)  RFLPs. 
D)  DNA ligation. 
E)  13 STR markers.

Question 11

Figure 15-3   The columns in the accompanying figure represent F (for female), C (for children), and M (for male). The presence of the band marked with arrow #2, shows that:

Accepted Answer

A)  this DNA sequence in the children is a mutation. 
B)  this DNA sequence in the children is inherited from their mother. 
C)  this DNA sequence in the children is inherited from their father. 
D)  this sequence in the children is inherited from both their mother and father. 
E)  individual F is not the mother of these children. 
A)  this DNA sequence in the children is a mutation. 
B)  this DNA sequence in the children is inherited from their mother. 
C)  this DNA sequence in the children is inherited from their father. 
D)  this sequence in the children is inherited from both their mother and father. 
E)  individual F is not the mother of these children.

Question 12

The ratio of different-colored fluorescent dyes in microarray can be used to infer information about:

Accepted Answer

A)  patterns of gene expression only. 
B)  drug activity only. 
C)  the identity of disease-causing genes only. 
D)  patterns of gene expression and also drug activity only. 
E)  patterns of gene expression, drug activity, and the identity of disease-causing genes. 
A)  patterns of gene expression only. 
B)  drug activity only. 
C)  the identity of disease-causing genes only. 
D)  patterns of gene expression and also drug activity only. 
E)  patterns of gene expression, drug activity, and the identity of disease-causing genes.

Question 13

Transformation is a process whereby:

Accepted Answer

A)  bacteria are transferred into plasmid cells. 
B)  viruses are transferred into bacterial cells. 
C)  plasmids are transferred into bacterial cells. 
D)  bacteria are transferred into viral cells. 
E)  plasmids are transferred into viral cells. 
A)  bacteria are transferred into plasmid cells. 
B)  viruses are transferred into bacterial cells. 
C)  plasmids are transferred into bacterial cells. 
D)  bacteria are transferred into viral cells. 
E)  plasmids are transferred into viral cells.

Question 14

A genomic library consists of fragments of all of the DNA:

Accepted Answer

A)  that occurs in one species. 
B)  contained in one chromosome. 
C)  that would be found in a typical cell of a particular organism. 
D)  in a recombinant gene. 
E)  that codes for functional enzymes in a typical cell of a particular organism. 
A)  that occurs in one species. 
B)  contained in one chromosome. 
C)  that would be found in a typical cell of a particular organism. 
D)  in a recombinant gene. 
E)  that codes for functional enzymes in a typical cell of a particular organism.

Question 15

GTGTGTGTGT is an example of a(n) single nucleotide polymorphism .

Accepted Answer

A) True 
 B)False

Question 16

Identify two important proteins that are produced using genetic engineering techniques. Briefly discuss why the genetic engineering techniques used are preferred over the other methods of producing each of the proteins that you identified.

Accepted Answer

Here are three possible answers: 
1. Ins

Question 17

How does a cDNA library differ from a genomic library? How are cDNA libraries significant to the production of eukaryotic gene products?

Accepted Answer

cDNA libraries contain DNA that was prod

Question 18

A detectable variation in the genomes of different individuals of a population is known as:

Accepted Answer

A)  DNA sequencing. 
B)  DNA electrophoresis. 
C)  DNA tandem repeats. 
D)  a polymorphism. 
E)  DNA profiling. 
A)  DNA sequencing. 
B)  DNA electrophoresis. 
C)  DNA tandem repeats. 
D)  a polymorphism. 
E)  DNA profiling.

Question 19

In the construction of a DNA library, bacterial colonies that grow on agar plates containing antibiotic can be described as:

Accepted Answer

A)  arising from one individual bacterium that contained a recombinant plasmid. 
B)  arising from many individual bacteria, only some of which contain a recombinant plasmid 
C)  arising from one individual bacterium that does not contain a recombinant plasmid. 
D)  arising from wild-type bacteria. 
E)  containing a single bacterium, which contains a recombinant plasmid. 
A)  arising from one individual bacterium that contained a recombinant plasmid. 
B)  arising from many individual bacteria, only some of which contain a recombinant plasmid 
C)  arising from one individual bacterium that does not contain a recombinant plasmid. 
D)  arising from wild-type bacteria. 
E)  containing a single bacterium, which contains a recombinant plasmid.

Question 20

The use of organisms to develop useful products is called:

Accepted Answer

A)  bioengineering. 
B)  in vitro gene technology. 
C)  biotechnology. 
D)  recombinant DNA technology. 
E)  genetic engineering. 
A)  bioengineering. 
B)  in vitro gene technology. 
C)  biotechnology. 
D)  recombinant DNA technology. 
E)  genetic engineering.

Exam 15: Dna Technology and Genomics

If a protein-coding gene is identified, its function can be studied by using RNA interference to ____.

An organism in which foreign genes have been incorporated is called a:

The chain termination method of DNA sequencing is based on a replicating DNA strand incorporating a(n) deoxynucleotide that stops the chain elongation. __________________

A genomic library is a collection of:

When DNA ligase is used to combine a human fragment with a plasmid, the result is a(n) redundant plasmid. __________________

Splicing together DNA from two different organisms is known as:

What is one feature of "engineered" plasmids that is helpful in the isolation and analysis of cloned DNA?

What purpose do restriction enzymes play in bacterial cells?

Scientists study animal models in order to reveal the functions of human genes. This is an example of:

The FBI currently determines an individual's DNA profile using:

Figure 15-3 The columns in the accompanying figure represent F (for female), C (for children), and M (for male). The presence of the band marked with arrow #2, shows that:

The ratio of different-colored fluorescent dyes in microarray can be used to infer information about:

Transformation is a process whereby:

A genomic library consists of fragments of all of the DNA:

GTGTGTGTGT is an example of a(n) single nucleotide polymorphism .

Identify two important proteins that are produced using genetic engineering techniques. Briefly discuss why the genetic engineering techniques used are preferred over the other methods of producing each of the proteins that you identified.

How does a cDNA library differ from a genomic library? How are cDNA libraries significant to the production of eukaryotic gene products?

A detectable variation in the genomes of different individuals of a population is known as:

In the construction of a DNA library, bacterial colonies that grow on agar plates containing antibiotic can be described as:

The use of organisms to develop useful products is called:

A View of Life

Atoms and Molecules the Chemical Basis of Life

The Chemistry of Life Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp Energy-Releasing Pathways

Photosynthesis Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

Dna the Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure Growth and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function an Introduction

Protection Support and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

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