Question 1

Suppose a scientist is interested in studying DNA regulatory elements. What should she prepare first?

Accepted Answer

A)  cDNA library 
B)  genomic library 
C)  transgenic mouse 
D)  Northern blot 
E)  DNA chip 
A)  cDNA library 
B)  genomic library 
C)  transgenic mouse 
D)  Northern blot 
E)  DNA chip

Question 2

In producing a genomic library, human DNA and plasmid DNA must first be treated with:

Accepted Answer

A)  the same restriction enzyme. 
B)  different restriction enzymes. 
C)  the same DNA ligase. 
D)  different DNA ligases. 
E)  cosmid cloning vectors 
A)  the same restriction enzyme. 
B)  different restriction enzymes. 
C)  the same DNA ligase. 
D)  different DNA ligases. 
E)  cosmid cloning vectors

Question 3

PCR has many uses and advantages. What is one disadvantage of this technology?

Accepted Answer

A)  It is difficult to keep the amplified DNA strands intact. 
B)  PCR works on only some DNA sequences. 
C)  The amplified product is often degraded. 
D)  PCR is a slow and inefficient process. 
E)  PCR is easily contaminated with foreign DNA. 
A)  It is difficult to keep the amplified DNA strands intact. 
B)  PCR works on only some DNA sequences. 
C)  The amplified product is often degraded. 
D)  PCR is a slow and inefficient process. 
E)  PCR is easily contaminated with foreign DNA.

Question 4

If a human gene containing introns is inserted into a prokaryote cell, a(n) functional human protein will be produced. 
__________________

Accepted Answer

A) True 
 B)False

Question 5

Single nucleotide polymorphisms are defined as junk DNA .

Accepted Answer

A) True 
 B)False

Question 6

Cosmid cloning vectors accommodate much larger fragments of DNA than bacterial artificial chromosomes. 
__________________

Accepted Answer

A) True 
 B)False

Question 7

Some restriction enzymes create blunt DNA ends, without the complementary single-stranded overhangs of sticky ends. What is the advantage of using restriction enzymes that create sticky ends vs. blunt ends for creating recombinant DNA molecules?

Accepted Answer

Since sticky ends are complementary, the

Question 8

Match between columns
                        Premises: gene expression; protein products
gene expression; single mRNA transcripts
genome-wide gene expression
compare DNA or protein sequences
DNA sequences

Accepted Answer

The Answer of Southern blot and Western blot and Northern is...

Question 9

Explain how DNA sequencing can be accomplished using dideoxynucleotides.

Accepted Answer

Four reaction mixtures must be prepared,

Question 10

After a protein coding gene is identified, its function can be studied by using RNA interference to activate the gene.

Accepted Answer

A) True 
 B)False

Question 11

Why is insulin produced by genetically engineered E. coli cells superior to insulin obtained from animal sources?

Accepted Answer

A)  Animal insulin has a shorter life span than insulin produced using recombinant DNA techniques. 
B)  Recombinantly produced insulin contains human rather than animal sequences, reducing the chances of an allergic response. 
C)  Animal insulin is more difficult to purify than recombinantly produced insulin. 
D)  Recombinantly produced insulin has a much higher activity level. 
E)  Recombinantly produced insulin is easier to obtain than insulin from animals. 
A)  Animal insulin has a shorter life span than insulin produced using recombinant DNA techniques. 
B)  Recombinantly produced insulin contains human rather than animal sequences, reducing the chances of an allergic response. 
C)  Animal insulin is more difficult to purify than recombinantly produced insulin. 
D)  Recombinantly produced insulin has a much higher activity level. 
E)  Recombinantly produced insulin is easier to obtain than insulin from animals.

Question 12

A ____ is required to transfer genes from one organism to another.

Accepted Answer

A)  vector 
B)  reverse transcriptase 
C)  transport molecule 
D)  genetic probe 
E)  PCR device 
A)  vector 
B)  reverse transcriptase 
C)  transport molecule 
D)  genetic probe 
E)  PCR device

Question 13

Which of the following statements characterizes PCR?

Accepted Answer

A)  PCR can be performed with any DNA polymerase. 
B)  PCR is only useful for amplifying large quantities of DNA in vitro . 
C)  PCR replicates specific DNA sequences. 
D)  PCR has not been successful for archaeological and crime scene analyses. 
E)  PCR utilizes Taq polymerase, which active only at low temperatures. 
A)  PCR can be performed with any DNA polymerase. 
B)  PCR is only useful for amplifying large quantities of DNA in vitro . 
C)  PCR replicates specific DNA sequences. 
D)  PCR has not been successful for archaeological and crime scene analyses. 
E)  PCR utilizes Taq polymerase, which active only at low temperatures.

Question 14

In the field of plant transgenics, the use of the bacterium Agrobacterium tumefaciens has proven useful because the bacterium:

Accepted Answer

A)  is not attacked by plant immune systems. 
B)  has been genetically engineered to penetrate the plant cell wall. 
C)  can directly modify the plant genome. 
D)  is found naturally in almost all species of plants. 
E)  is able to transfer a plasmid into host plant cells. 
A)  is not attacked by plant immune systems. 
B)  has been genetically engineered to penetrate the plant cell wall. 
C)  can directly modify the plant genome. 
D)  is found naturally in almost all species of plants. 
E)  is able to transfer a plasmid into host plant cells.

Question 15

Why is human blood clotting factor VIII produced by genetically engineered bacteria better to treat hemophilia type A than the clotting factor isolated from human or animal blood?

Accepted Answer

A)  Recombinantly produced clotting factor has a longer shelf life than natural clotting factor. 
B)  Clotting factor isolated from blood can transmit infectious agents present in the donated blood. 
C)  Clotting factor isolated from human blood is quickly degraded. 
D)  Recombinantly produced clotting factor is stable at high temperatures. 
E)  Clotting factor from blood has a lower activity than recombinant clotting factor. 
A)  Recombinantly produced clotting factor has a longer shelf life than natural clotting factor. 
B)  Clotting factor isolated from blood can transmit infectious agents present in the donated blood. 
C)  Clotting factor isolated from human blood is quickly degraded. 
D)  Recombinantly produced clotting factor is stable at high temperatures. 
E)  Clotting factor from blood has a lower activity than recombinant clotting factor.

Question 16

DNA ligase links two ____ DNA fragments by ____ bonds.

Accepted Answer

A)  complementary; hydrogen 
B)  circular; covalent 
C)  palindromic; covalent 
D)  linear; covalent 
E)  linear; hydrogen 
A)  complementary; hydrogen 
B)  circular; covalent 
C)  palindromic; covalent 
D)  linear; covalent 
E)  linear; hydrogen

Question 17

Comparative genomics is the field of genomics that studies the functions of genes and nongene sequences in genomes.

Accepted Answer

A) True 
 B)False

Question 18

A large number of copies of any DNA segment can be obtained by:

Accepted Answer

A)  introducing foreign DNA into a microorganism so that it can be replicated. 
B)  stimulating increased transcription of the appropriate sequence of mRNA. 
C)  stimulating increased translation of the appropriate DNA molecule. 
D)  inducing the production of DNA from proteins. 
E)  stimulating reverse transcription of tRNA. 
A)  introducing foreign DNA into a microorganism so that it can be replicated. 
B)  stimulating increased transcription of the appropriate sequence of mRNA. 
C)  stimulating increased translation of the appropriate DNA molecule. 
D)  inducing the production of DNA from proteins. 
E)  stimulating reverse transcription of tRNA.

Question 19

Which DNA sequence below would be a palindromic sequence?

Accepted Answer

A)  AGCGCT 
B)  AGCAGC 
C)  AAAGGG 
D)  ACACAC 
E)  AGTCGT 
A)  AGCGCT 
B)  AGCAGC 
C)  AAAGGG 
D)  ACACAC 
E)  AGTCGT

Question 20

To produce a genomic library DNA, DNA fragments are incorporated into plasmids that are introduced into bacteria . 
__________________

Accepted Answer

A) True 
 B)False

Exam 15: Dna Technology and Genomics

Suppose a scientist is interested in studying DNA regulatory elements. What should she prepare first?

In producing a genomic library, human DNA and plasmid DNA must first be treated with:

PCR has many uses and advantages. What is one disadvantage of this technology?

If a human gene containing introns is inserted into a prokaryote cell, a(n) functional human protein will be produced. __________________

Single nucleotide polymorphisms are defined as junk DNA .

Cosmid cloning vectors accommodate much larger fragments of DNA than bacterial artificial chromosomes. __________________

Some restriction enzymes create blunt DNA ends, without the complementary single-stranded overhangs of sticky ends. What is the advantage of using restriction enzymes that create sticky ends vs. blunt ends for creating recombinant DNA molecules?

Explain how DNA sequencing can be accomplished using dideoxynucleotides.

After a protein coding gene is identified, its function can be studied by using RNA interference to activate the gene.

Why is insulin produced by genetically engineered E. coli cells superior to insulin obtained from animal sources?

A ____ is required to transfer genes from one organism to another.

Which of the following statements characterizes PCR?

In the field of plant transgenics, the use of the bacterium Agrobacterium tumefaciens has proven useful because the bacterium:

Why is human blood clotting factor VIII produced by genetically engineered bacteria better to treat hemophilia type A than the clotting factor isolated from human or animal blood?

DNA ligase links two ____ DNA fragments by ____ bonds.

Comparative genomics is the field of genomics that studies the functions of genes and nongene sequences in genomes.

A large number of copies of any DNA segment can be obtained by:

Which DNA sequence below would be a palindromic sequence?

To produce a genomic library DNA, DNA fragments are incorporated into plasmids that are introduced into bacteria . __________________

A View of Life

Atoms and Molecules the Chemical Basis of Life

The Chemistry of Life Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp Energy-Releasing Pathways

Photosynthesis Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

Dna the Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure Growth and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function an Introduction

Protection Support and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

Filters

DNA ligase links two DNA fragments by bonds.