Question 1

Which objective lens is used to count erythrocytes in the four corner squares and the center square of the primary center square of a hemacytometer?

Accepted Answer

A)  x10 
B)  x40 
C)  x100 
D)  x4 
E)  None of the above 
A)  x10 
B)  x40 
C)  x100 
D)  x4 
E)  None of the above

Question 2

A laboratory technologist sets up a sickle screen for a patient according to procedure. When the technologist goes to read the results, he notices that he cannot see the black lines on the reading card through the patient's solution. QC was performed with the patient's sample and the technologist could see the black lines on the negative control but could not see them on the positive control. What should the technician do now?

Accepted Answer

A)  Report the result as positive for hemoglobin S. 
B)  Report the result as negative for hemoglobin S. 
C)  Report the result as negative. 
D)  Report the result as positive. 
E)  Repeat the test. 
A)  Report the result as positive for hemoglobin S. 
B)  Report the result as negative for hemoglobin S. 
C)  Report the result as negative. 
D)  Report the result as positive. 
E)  Repeat the test.

Question 3

How is hereditary erythroblastic multinuclearity with positive acidified serum (HEMPAS) test differentiated from PNH patients in the Ham's test?

Accepted Answer

A)  In HEMPAS, RBCs are not lysed by the patient's own acidified serum. 
B)  In HEMPAS, the sugar water test is positive. 
C)  In HEMPAS, complement affixes to RBCs at a basic pH. 
D)  All of the above 
E)  Two of the above 
A)  In HEMPAS, RBCs are not lysed by the patient's own acidified serum. 
B)  In HEMPAS, the sugar water test is positive. 
C)  In HEMPAS, complement affixes to RBCs at a basic pH. 
D)  All of the above 
E)  Two of the above

Question 4

If when performing an osmotic fragility test the plasma is moderately jaundiced, the plasma should be replaced with __________.

Accepted Answer

A)  Distilled water 
B)  Dextran 
C)  Isotonic saline 
D)  All of the above 
E)  None of the above 
A)  Distilled water 
B)  Dextran 
C)  Isotonic saline 
D)  All of the above 
E)  None of the above

Question 5

In the slide-to-slide peripheral blood smear, the _________ tend to reside in the middle of the feathered edge.

Accepted Answer

A)  Monocytes 
B)  Lymphocytes 
C)  Eosinophils 
D)  Basophils 
E)  Neutrophils 
A)  Monocytes 
B)  Lymphocytes 
C)  Eosinophils 
D)  Basophils 
E)  Neutrophils

Question 6

The normal platelet count range is ________.

Accepted Answer

A)  50-100 F1F1F1S1F1F1F10 109/L 
B)  200-450 F1F1F1S1F1F1F10 109/L 
C)  300-500 F1F1F1S1F1F1F10 109/L 
D)  130-400 F1F1F1S1F1F1F10 109/L 
E)  None of the above 
A)  50-100 F1F1F1S1F1F1F10 109/L 
B)  200-450 F1F1F1S1F1F1F10 109/L 
C)  300-500 F1F1F1S1F1F1F10 109/L 
D)  130-400 F1F1F1S1F1F1F10 109/L 
E)  None of the above

Question 7

When measuring the hematocrit, the RBCs are separated from plasma during centrifugation, rendering a white band consisting of WBCs and platelets. This band is termed the __________ .

Accepted Answer

A)  Lecithin 
B)  Buffy coat 
C)  Red cell interface 
D)  Plasma interface 
E)  None of the above 
A)  Lecithin 
B)  Buffy coat 
C)  Red cell interface 
D)  Plasma interface 
E)  None of the above

Question 8

Which procedure can be used to separate HbA2 from HbE?

Accepted Answer

A)  Cellulose acetate hemoglobin electrophoresis 
B)  HbA2 by anion-exchange chromatography 
C)  Citrate agar hemoglobin electrophoresis 
D)  All of the above 
E)  None of the above 
A)  Cellulose acetate hemoglobin electrophoresis 
B)  HbA2 by anion-exchange chromatography 
C)  Citrate agar hemoglobin electrophoresis 
D)  All of the above 
E)  None of the above

Question 9

What is the diluent used when counting platelets by the Unopette method?

Accepted Answer

A)  Ammonium oxalate 
B)  Hayem's solution 
C)  Gower's solution 
D)  Acetic acid 
E)  None of the above 
A)  Ammonium oxalate 
B)  Hayem's solution 
C)  Gower's solution 
D)  Acetic acid 
E)  None of the above

Question 10

Which anticoagulant is recommended for correcting manual platelet counts that exhibit "platelet satellitosis"?

Accepted Answer

A)  Sodium citrate 
B)  Sodium oxalate 
C)  Sodium fluoride 
D)  Lithium heparin 
E)  None of the above 
A)  Sodium citrate 
B)  Sodium oxalate 
C)  Sodium fluoride 
D)  Lithium heparin 
E)  None of the above

Question 11

________ is defined as the volume occupied by erythrocytes in a given volume of blood and is usually expressed as a percentage of the volume of the whole blood sample.

Accepted Answer

A)  Hemoglobin 
B)  Hematocrit 
C)  ESR 
D)  RDW 
E)  None of the above 
A)  Hemoglobin 
B)  Hematocrit 
C)  ESR 
D)  RDW 
E)  None of the above

Question 12

When counting cells in a hemacytometer, cells inside a square with a double boundary line should be counted as well as:

Accepted Answer

A)  Cells touching the middle inward line 
B)  Cells touching the innermost line 
C)  Artifacts 
D)  All of the above 
E)  None of the above 
A)  Cells touching the middle inward line 
B)  Cells touching the innermost line 
C)  Artifacts 
D)  All of the above 
E)  None of the above

Question 13

Manual cell counts are frequently performed on patients with extreme cases of:

Accepted Answer

A)  Thrombocytosis 
B)  Thrombocytopenia 
C)  Leukocytosis 
D)  Leukopenia 
E)  All of the above 
A)  Thrombocytosis 
B)  Thrombocytopenia 
C)  Leukocytosis 
D)  Leukopenia 
E)  All of the above

Question 14

HbF is ________ to acid elution.

Accepted Answer

A)  Sensitive 
B)  Resistant 
C)  Hypotonic 
D)  All of the above 
E)  None of the above 
A)  Sensitive 
B)  Resistant 
C)  Hypotonic 
D)  All of the above 
E)  None of the above

Question 15

What is the basic principle of cyanmethemoglobin determination of hemoglobin?

Accepted Answer

A)  Cyanmethemoglobin oxidizes hemoglobin to methemoglobin, which is converted to potassium cyanide. 
B)  Potassium cyanide oxidizes hemoglobin to methemoglobin, which is converted to cyanmethemoglobin. 
C)  Potassium ferricyanide oxidizes hemoglobin to methemoglobin, which is converted to cyanmethemoglobin by potassium cyanide. 
D)  All of the above 
E)  None of the above 
A)  Cyanmethemoglobin oxidizes hemoglobin to methemoglobin, which is converted to potassium cyanide. 
B)  Potassium cyanide oxidizes hemoglobin to methemoglobin, which is converted to cyanmethemoglobin. 
C)  Potassium ferricyanide oxidizes hemoglobin to methemoglobin, which is converted to cyanmethemoglobin by potassium cyanide. 
D)  All of the above 
E)  None of the above

Question 16

What is meant by the term "universal precautions?"

Accepted Answer

A)  Wear gloves only when a known infected specimen needs processing. 
B)  Handle all specimens as if they were infected with hepatitis or HIV. 
C)  Wear the same pair of gloves between patients when performing venipuncture. 
D)  All of the above 
E)  None of the above 
A)  Wear gloves only when a known infected specimen needs processing. 
B)  Handle all specimens as if they were infected with hepatitis or HIV. 
C)  Wear the same pair of gloves between patients when performing venipuncture. 
D)  All of the above 
E)  None of the above

Question 17

The normal RBC range for an adult male is ________.

Accepted Answer

A)  4.2-5.4 F1F1F1S1F1F1F10 1012/L 
B)  4.4-5.8 F1F1F1S1F1F1F10 1012/L 
C)  4.7-6.1 F1F1F1S1F1F1F10 1012/L 
D)  3.8-5.5 F1F1F1S1F1F1F10 1012/L 
E)  None of the above 
A)  4.2-5.4 F1F1F1S1F1F1F10 1012/L 
B)  4.4-5.8 F1F1F1S1F1F1F10 1012/L 
C)  4.7-6.1 F1F1F1S1F1F1F10 1012/L 
D)  3.8-5.5 F1F1F1S1F1F1F10 1012/L 
E)  None of the above

Question 18

In the acid elution procedure for HbF, cells containing HbF will stain a ________.

Accepted Answer

A)  Light pink 
B)  Dark pink-red 
C)  Orange-red 
D)  Orange-yellow 
E)  None of the above 
A)  Light pink 
B)  Dark pink-red 
C)  Orange-red 
D)  Orange-yellow 
E)  None of the above

Question 19

An example of a blood dilution vial that is used for manual cell counts of white blood cells (WBCs), platelets, red blood cells (RBCs), eosinophils, and hemoglobin is the _______.

Accepted Answer

A)  Unopette 
B)  Westergren 
C)  Hemacue 
D)  Wintrobe 
E)  None of the above 
A)  Unopette 
B)  Westergren 
C)  Hemacue 
D)  Wintrobe 
E)  None of the above

Question 20

Using the Unopette system, the technologist counted an average of 25 leukocytes on a hemacytometer. What is the WBC count?

Accepted Answer

A)  2800/mm3 
B)  2000/mm3 
C)  5000/mm3 
D)  5600/mm3 
E)  None of the above 
A)  2800/mm3 
B)  2000/mm3 
C)  5000/mm3 
D)  5600/mm3 
E)  None of the above

Exam 31: Hematology Methods

Which objective lens is used to count erythrocytes in the four corner squares and the center square of the primary center square of a hemacytometer?

How is hereditary erythroblastic multinuclearity with positive acidified serum (HEMPAS) test differentiated from PNH patients in the Ham's test?

If when performing an osmotic fragility test the plasma is moderately jaundiced, the plasma should be replaced with __________.

In the slide-to-slide peripheral blood smear, the _________ tend to reside in the middle of the feathered edge.

The normal platelet count range is ________.

When measuring the hematocrit, the RBCs are separated from plasma during centrifugation, rendering a white band consisting of WBCs and platelets. This band is termed the __________ .

Which procedure can be used to separate HbA2 from HbE?

What is the diluent used when counting platelets by the Unopette method?

Which anticoagulant is recommended for correcting manual platelet counts that exhibit "platelet satellitosis"?

________ is defined as the volume occupied by erythrocytes in a given volume of blood and is usually expressed as a percentage of the volume of the whole blood sample.

When counting cells in a hemacytometer, cells inside a square with a double boundary line should be counted as well as:

Manual cell counts are frequently performed on patients with extreme cases of:

HbF is ________ to acid elution.

What is the basic principle of cyanmethemoglobin determination of hemoglobin?

What is meant by the term "universal precautions?"

The normal RBC range for an adult male is ________.

In the acid elution procedure for HbF, cells containing HbF will stain a ________.

An example of a blood dilution vial that is used for manual cell counts of white blood cells (WBCs), platelets, red blood cells (RBCs), eosinophils, and hemoglobin is the _______.

Using the Unopette system, the technologist counted an average of 25 leukocytes on a hemacytometer. What is the WBC count?

Morphology of Human Blood and Marrow Cells: Hematopoiesis

Bone Marrow

The Red Blood Cell: Structure and Function

Anemia: Diagnosis and Clinical Considerations

Evaluation of Red Blood Cell Morphology and Introduction to Platelet and White Blood Cell Morphology

Iron Metabolism and Hypochromic Anemias

Megaloblastic Anemias

Aplastic Anemia Including Pure Red Cell Aplasia and Congenital Dyserythropoietic Anemia and Paroxysmal Nocturnal Hemoglobinuria

Introduction to Hemolytic Anemias: Intracorpuscular Defects: I Hereditary Defects of the Red Cell Membrane

Hemolytic Anemias: Intracorpuscular Defects: II Hereditary Enzyme Deficiencies

Hemolytic Anemias: Intracorpuscular Defects: III the Hemoglobinopathies

Hemolytic Anemias: Intracorpuscular Defects: Iv Thalassemia

Hemolytic Anemias: Extracorpuscular Defects

Hypoproliferative Anemia: Anemia Associated With Systemic Diseases

Cell Biology, Disorders of Neutrophils, Infectious Mononucleosis, and Reactive Lymphocytosis

Introduction to Leukemia and the Acute Leukemias

Chronic Myeloproliferative Disorders I: Chronic Myelogenous Leukemia

Chronic Myeloproliferative Disorders Ii: Polycythemia Vera, Essential Thrombocythemia, and Idiopathic Myelofibrosis

Myelodysplastic Syndromes

Chronic Lymphocytic Leukemia and Related Lymphoproliferative Disorders

The Lymphomas

Multiple Myeloma and Related Plasma Cell Disorders

Lipid Lysosomal Storage Diseases and Histiocytosis

Introduction to Hemostasis

Disorders of Primary Hemostasis: Quantitative and Qualitative Platelet Disorders and Vascular Disorders

Disorders of Plasma Clotting Factors

Interaction of the Fibrinolytic, Coagulation, and Kinin Systems; Disseminated Intravascular Coagulation; and Related Pathology

Introduction to Thrombosis and Anticoagulant Therapy

Quality Control in the Hematology Laboratory

Body Fluid Examination: the Qualitative, Quantitative, and Morphologic Analysis of Serous, Cerebrospinal, and Synovial Fluids

Principles of Automated Differential Analysis

Coagulation Procedures

Applications of Flow Cytometry to Hematology and Hemostasis

Molecular Diagnostic Techniques in Hematopathology

Special Stainscytochemistry

Filters

Which procedure can be used to separate HbA₂ from HbE?