Question 1

________ is an automated parameter provided by hematology instruments that indicates the degree of anisocytosis.

Accepted Answer

A)  MCV 
B)  Red cell distribution width (RDW) 
C)  Packed cell volume (PCV) 
D)  MCH 
E)  MCHC 
A)  MCV 
B)  Red cell distribution width (RDW) 
C)  Packed cell volume (PCV) 
D)  MCH 
E)  MCHC B

Question 2

Venipuncture is defined as:

Accepted Answer

A)  Blood collected from the capillary bed. 
B)  Blood collected from a vein. 
C)  Blood collected from an artery. 
D)  Blood collected from a venule. 
E)  None of the above. 
A)  Blood collected from the capillary bed. 
B)  Blood collected from a vein. 
C)  Blood collected from an artery. 
D)  Blood collected from a venule. 
E)  None of the above. B

Question 3

Blood smears are prepared with ____________ anticoagulated blood.

Accepted Answer

A)  Sodium citrate 
B)  EDTA 
C)  Sodium heparin 
D)  Sodium oxalate 
E)  None of the above 
A)  Sodium citrate 
B)  EDTA 
C)  Sodium heparin 
D)  Sodium oxalate 
E)  None of the above B

Question 4

What is the correct sequence of migration from cathode to anode in hemoglobin electrophoresis using citrate agar at acid pH?

Accepted Answer

A)  C, S, A1, A2, D, E, F 
B)  A1, S, C, D, A2, E, F 
C)  F, S, C, A2, A1, D, D 
D)  D, S, C, A2, A1, F, E 
E)  None of the above 
A)  C, S, A1, A2, D, E, F 
B)  A1, S, C, D, A2, E, F 
C)  F, S, C, A2, A1, D, D 
D)  D, S, C, A2, A1, F, E 
E)  None of the above

Question 5

A _______ ratio of whole blood to supravital stain is required for reticulocyte staining.

Accepted Answer

A)  1:5 
B)  1:1 
C)  1:10 
D)  2:1 
E)  None of the above 
A)  1:5 
B)  1:1 
C)  1:10 
D)  2:1 
E)  None of the above

Question 6

In patients with PNH, the sucrose solution in the sugar water test for PNH provides a low ionic strength environment that allows _______ to bind to the RBCs.

Accepted Answer

A)  IgM immunoglobulin 
B)  IgG immunoglobulin 
C)  Complement 
D)  All of the above 
E)  None of the above 
A)  IgM immunoglobulin 
B)  IgG immunoglobulin 
C)  Complement 
D)  All of the above 
E)  None of the above

Question 7

A normal RBC range for a newborn is _________.

Accepted Answer

A)  3.8-5.5 F1F1F1S1F1F1F10 1012/L 
B)  4.4-5.8 F1F1F1S1F1F1F10 1012/L 
C)  4.7-6.1 F1F1F1S1F1F1F10 1012/L 
D)  4.2-5.4 F1F1F1S1F1F1F10 1012/L 
E)  None of the above 
A)  3.8-5.5 F1F1F1S1F1F1F10 1012/L 
B)  4.4-5.8 F1F1F1S1F1F1F10 1012/L 
C)  4.7-6.1 F1F1F1S1F1F1F10 1012/L 
D)  4.2-5.4 F1F1F1S1F1F1F10 1012/L 
E)  None of the above

Question 8

An autohemolysis test demonstrates marked autohemolysis without the addition of dextrose. This is consistent with which hematologic disorder(s)?

Accepted Answer

A)  Hereditary spherocytosis 
B)  Glucose 6-phosphate dehydrogenase (G6P
D)  deficiency 
C)  Acquired spherocytic hemolytic anemia 
D)  Pyruvate kinase deficiency 
E)  All of the above 
A)  Hereditary spherocytosis 
B)  Glucose 6-phosphate dehydrogenase (G6P
D)  deficiency 
C)  Acquired spherocytic hemolytic anemia 
D)  Pyruvate kinase deficiency 
E)  All of the above

Question 9

Which disease is represented by the following laboratory data? RBC count 3.1 F1F1F1S1F1F1F10 1012/µL
Hgb 10.6 g/dL
Hct 32%

Accepted Answer

A)  Iron-deficiency anemia 
B)  Thalassemia minor 
C)  Polycythemia vera 
D)  Pernicious anemia 
E)  Anemia of chronic infection 
A)  Iron-deficiency anemia 
B)  Thalassemia minor 
C)  Polycythemia vera 
D)  Pernicious anemia 
E)  Anemia of chronic infection

Question 10

Calculate the MCV based on the following: hematocrit = 45%, RBC = 4.7 F1F1F1S1F1F1F10 106/F1F1F1S1F1F1F10L, hemoglobin = 15 g/dL.

Accepted Answer

A)  75 fL 
B)  96 fL 
C)  87 fL 
D)  100 fL 
E)  120 fL 
A)  75 fL 
B)  96 fL 
C)  87 fL 
D)  100 fL 
E)  120 fL

Question 11

When performing an osmotic fragility test, normal blood will demonstrate hemolysis at the ___________ concentration of saline.

Accepted Answer

A)  0.35% 
B)  0.45% 
C)  0.40% 
D)  0.20% 
E)  None of the above 
A)  0.35% 
B)  0.45% 
C)  0.40% 
D)  0.20% 
E)  None of the above

Question 12

The normal value for RDW is _____.

Accepted Answer

A)  5.0-12.5% 
B)  6.0-8.0% 
C)  11.5-14.5% 
D)  12.0-16.0% 
E)  None of the above 
A)  5.0-12.5% 
B)  6.0-8.0% 
C)  11.5-14.5% 
D)  12.0-16.0% 
E)  None of the above

Question 13

The range of normal values for reticulocytes in a newborn is:

Accepted Answer

A)  0.5-2.0% 
B)  2.5-6.0% 
C)  1.0-3.0% 
D)  3.0-5.0% 
E)  None of the above 
A)  0.5-2.0% 
B)  2.5-6.0% 
C)  1.0-3.0% 
D)  3.0-5.0% 
E)  None of the above

Question 14

Using the Unopette system, the technologist performed a manual platelet count. An average of 300 platelets were counted on a hemacytometer. What is the platelet count per liter?

Accepted Answer

A)  300,000 
B)  300 
C)  150,000 
D)  150 
E)  500 
A)  300,000 
B)  300 
C)  150,000 
D)  150 
E)  500

Question 15

The presence or absence of _________ is an effective measure of erythrocyte susceptibility to hypotonic damage.

Accepted Answer

A)  Jaundice 
B)  Hemolysis 
C)  Lipemia 
D)  Inclusion bodies 
E)  None of the above 
A)  Jaundice 
B)  Hemolysis 
C)  Lipemia 
D)  Inclusion bodies 
E)  None of the above

Question 16

When counting cells on a hemacytometer, the four corner and center secondary square of the center primary square are used to count _______.

Accepted Answer

A)  Erythrocytes 
B)  Leukocytes 
C)  Platelets 
D)  Basophils 
E)  None of the above 
A)  Erythrocytes 
B)  Leukocytes 
C)  Platelets 
D)  Basophils 
E)  None of the above

Question 17

Perform a corrected WBC count given the following: WBC = 30,000/ mm3, nucleated RBCs = 12/100 WBCs.

Accepted Answer

A)  15.2 F1F1F1S1F1F1F10 103/mm3 
B)  35.0 F1F1F1S1F1F1F10 103/mm3 
C)  26.7 F1F1F1S1F1F1F10 103/mm3 
D)  21.2 F1F1F1S1F1F1F10 103/mm3 
E)  None of the above 
A)  15.2 F1F1F1S1F1F1F10 103/mm3 
B)  35.0 F1F1F1S1F1F1F10 103/mm3 
C)  26.7 F1F1F1S1F1F1F10 103/mm3 
D)  21.2 F1F1F1S1F1F1F10 103/mm3 
E)  None of the above

Question 18

What is the principle of the acidified serum test (Ham's test)?

Accepted Answer

A)  IgM immunoglobulin affixes to RBCs, the classic complement pathway is activated, and the red cells are lysed. 
B)  IgG immunoglobulin affixes to RBCs, the alternate pathway is activated, and the RBC is lysed. 
C)  Complement affixes to RBCs at acid pH, the alternate pathway is activated, and red cell lysis occurs. 
D)  All of the above 
E)  None of the above 
A)  IgM immunoglobulin affixes to RBCs, the classic complement pathway is activated, and the red cells are lysed. 
B)  IgG immunoglobulin affixes to RBCs, the alternate pathway is activated, and the RBC is lysed. 
C)  Complement affixes to RBCs at acid pH, the alternate pathway is activated, and red cell lysis occurs. 
D)  All of the above 
E)  None of the above

Question 19

Stress reticulocytes can be differentiated from mature reticulocytes by:

Accepted Answer

A)  Filamentous reticulum 
B)  Presence of polychromatophilic macrocytes on the peripheral smear 
C)  Presence of microcytes on the peripheral smear 
D)  All of the above 
E)  None of the above 
A)  Filamentous reticulum 
B)  Presence of polychromatophilic macrocytes on the peripheral smear 
C)  Presence of microcytes on the peripheral smear 
D)  All of the above 
E)  None of the above

Question 20

Platelet counts performed using the Unopette system will appear as round, oval, or rod-like and sometimes showing dendritic processes. In contrast, red cells will appear as:

Accepted Answer

A)  Eccentric cells 
B)  Bipolar cells 
C)  Mall disc-like cells 
D)  Ghost cells 
E)  None of the above 
A)  Eccentric cells 
B)  Bipolar cells 
C)  Mall disc-like cells 
D)  Ghost cells 
E)  None of the above

Exam 31: Hematology Methods

________ is an automated parameter provided by hematology instruments that indicates the degree of anisocytosis.

Venipuncture is defined as:

Blood smears are prepared with ____________ anticoagulated blood.

What is the correct sequence of migration from cathode to anode in hemoglobin electrophoresis using citrate agar at acid pH?

A _______ ratio of whole blood to supravital stain is required for reticulocyte staining.

In patients with PNH, the sucrose solution in the sugar water test for PNH provides a low ionic strength environment that allows _______ to bind to the RBCs.

A normal RBC range for a newborn is _________.

An autohemolysis test demonstrates marked autohemolysis without the addition of dextrose. This is consistent with which hematologic disorder(s)?

Which disease is represented by the following laboratory data? RBC count 3.1  1012/µL Hgb 10.6 g/dL Hct 32%

Calculate the MCV based on the following: hematocrit = 45%, RBC = 4.7  106/L, hemoglobin = 15 g/dL.

When performing an osmotic fragility test, normal blood will demonstrate hemolysis at the ___________ concentration of saline.

The normal value for RDW is _____.

The range of normal values for reticulocytes in a newborn is:

Using the Unopette system, the technologist performed a manual platelet count. An average of 300 platelets were counted on a hemacytometer. What is the platelet count per liter?

The presence or absence of _________ is an effective measure of erythrocyte susceptibility to hypotonic damage.

When counting cells on a hemacytometer, the four corner and center secondary square of the center primary square are used to count _______.

Perform a corrected WBC count given the following: WBC = 30,000/ mm3, nucleated RBCs = 12/100 WBCs.

What is the principle of the acidified serum test (Ham's test)?

Stress reticulocytes can be differentiated from mature reticulocytes by:

Platelet counts performed using the Unopette system will appear as round, oval, or rod-like and sometimes showing dendritic processes. In contrast, red cells will appear as:

Morphology of Human Blood and Marrow Cells: Hematopoiesis

Bone Marrow

The Red Blood Cell: Structure and Function

Anemia: Diagnosis and Clinical Considerations

Evaluation of Red Blood Cell Morphology and Introduction to Platelet and White Blood Cell Morphology

Iron Metabolism and Hypochromic Anemias

Megaloblastic Anemias

Aplastic Anemia Including Pure Red Cell Aplasia and Congenital Dyserythropoietic Anemia and Paroxysmal Nocturnal Hemoglobinuria

Introduction to Hemolytic Anemias: Intracorpuscular Defects: I Hereditary Defects of the Red Cell Membrane

Hemolytic Anemias: Intracorpuscular Defects: II Hereditary Enzyme Deficiencies

Hemolytic Anemias: Intracorpuscular Defects: III the Hemoglobinopathies

Hemolytic Anemias: Intracorpuscular Defects: Iv Thalassemia

Hemolytic Anemias: Extracorpuscular Defects

Hypoproliferative Anemia: Anemia Associated With Systemic Diseases

Cell Biology, Disorders of Neutrophils, Infectious Mononucleosis, and Reactive Lymphocytosis

Introduction to Leukemia and the Acute Leukemias

Chronic Myeloproliferative Disorders I: Chronic Myelogenous Leukemia

Chronic Myeloproliferative Disorders Ii: Polycythemia Vera, Essential Thrombocythemia, and Idiopathic Myelofibrosis

Myelodysplastic Syndromes

Chronic Lymphocytic Leukemia and Related Lymphoproliferative Disorders

The Lymphomas

Multiple Myeloma and Related Plasma Cell Disorders

Lipid Lysosomal Storage Diseases and Histiocytosis

Introduction to Hemostasis

Disorders of Primary Hemostasis: Quantitative and Qualitative Platelet Disorders and Vascular Disorders

Disorders of Plasma Clotting Factors

Interaction of the Fibrinolytic, Coagulation, and Kinin Systems; Disseminated Intravascular Coagulation; and Related Pathology

Introduction to Thrombosis and Anticoagulant Therapy

Quality Control in the Hematology Laboratory

Body Fluid Examination: the Qualitative, Quantitative, and Morphologic Analysis of Serous, Cerebrospinal, and Synovial Fluids

Principles of Automated Differential Analysis

Coagulation Procedures

Applications of Flow Cytometry to Hematology and Hemostasis

Molecular Diagnostic Techniques in Hematopathology

Special Stainscytochemistry

Filters

Which disease is represented by the following laboratory data? RBC count 3.1  10¹²/µL Hgb 10.6 g/dL Hct 32%

Calculate the MCV based on the following: hematocrit = 45%, RBC = 4.7  10⁶/L, hemoglobin = 15 g/dL.

Perform a corrected WBC count given the following: WBC = 30,000/ mm³, nucleated RBCs = 12/100 WBCs.