Question 1

A gene whose alteration is responsible for cancer formation is called a(n) _________.

Accepted Answer

A)  Heavy chain gene 
B)  Oncogene 
C)  Light chain gene 
D)  Pseudogene 
E)  None of the above 
A)  Heavy chain gene 
B)  Oncogene 
C)  Light chain gene 
D)  Pseudogene 
E)  None of the above

Question 2

Which of the following is/are major advantages of PCR?

Accepted Answer

A)  Extremely sensitive to low numbers of target sequences 
B)  Small sample size requirements 
C)  Relatively fast and inexpensive unless extensive post-amplification analysis is required 
D)  Permits analysis of formalin-fixed, paraffin-embedded tissue that is not suitable for Southern blot analysis 
E)  All of the above 
A)  Extremely sensitive to low numbers of target sequences 
B)  Small sample size requirements 
C)  Relatively fast and inexpensive unless extensive post-amplification analysis is required 
D)  Permits analysis of formalin-fixed, paraffin-embedded tissue that is not suitable for Southern blot analysis 
E)  All of the above

Question 3

DNA or RNA is readily extracted from which of the following specimens?

Accepted Answer

A)  Blood 
B)  Marrow 
C)  Body fluid cells 
D)  Tissue samples 
E)  All of the above 
A)  Blood 
B)  Marrow 
C)  Body fluid cells 
D)  Tissue samples 
E)  All of the above

Question 4

The restriction endonuclease HindIII is derived from which of the following organisms?

Accepted Answer

A)  Escherichia coli 
B)  Bacillus amyloliquifaciens 
C)  Haemophilus influenzae 
D)  Streptococcus pneumoniae 
E)  Staphylococcus aureus 
A)  Escherichia coli 
B)  Bacillus amyloliquifaciens 
C)  Haemophilus influenzae 
D)  Streptococcus pneumoniae 
E)  Staphylococcus aureus

Question 5

Which of the following is/are probe label(s)?

Accepted Answer

A)  Radioisotopes 
B)  Biotin 
C)  Digoxigenin 
D)  All of the above 
E)  None of the above 
A)  Radioisotopes 
B)  Biotin 
C)  Digoxigenin 
D)  All of the above 
E)  None of the above

Question 6

Which of the following statements describe a probe as used in molecular diagnostics?

Accepted Answer

A)  A single-stranded segment of nucleic acid (either DNA or RN
A)  whose nucleotide sequence is complementary to a target sequence (either DNA or RN
A) . 
B)  A double-stranded segment of nucleic acid (either DNA or RN
A)  whose nucleotide sequence is complementary to a target sequence (either DNA or RN
A) . 
C)  A single-stranded segment of amino acids encoded by a target sequence (either DNA or RN
A) . 
D)  All of the above. 
A)  A single-stranded segment of nucleic acid (either DNA or RN
A)  whose nucleotide sequence is complementary to a target sequence (either DNA or RN
A) . 
B)  A double-stranded segment of nucleic acid (either DNA or RN
A)  whose nucleotide sequence is complementary to a target sequence (either DNA or RN
A) . 
C)  A single-stranded segment of amino acids encoded by a target sequence (either DNA or RN
A) . 
D)  All of the above.

Question 7

DNA extracted from fresh cells or frozen tissue is resuspended in buffer and can be stored indefinitely at what temperature prior to amplification?

Accepted Answer

A)  0°C 
B)  4°C 
C)  20°C 
D)  -20°C 
E)  -10°C 
A)  0°C 
B)  4°C 
C)  20°C 
D)  -20°C 
E)  -10°C

Question 8

DNA and RNA are quantitated spectrophotometrically because nucleic acids absorb ultraviolet light at what optical density?

Accepted Answer

A)  200 nm 
B)  260 nm 
C)  280 nm 
D)  320 nm 
E)  400 nm 
A)  200 nm 
B)  260 nm 
C)  280 nm 
D)  320 nm 
E)  400 nm

Question 9

Intact DNA is usually well preserved in blood samples that are less than how many days old?

Accepted Answer

A)  10 days 
B)  7 days 
C)  6 days 
D)  4 days 
E)  2 days 
A)  10 days 
B)  7 days 
C)  6 days 
D)  4 days 
E)  2 days

Question 10

Which substance acts as an intermediary to DNA in transmitting genetic information?

Accepted Answer

A)  Oncogenes 
B)  Chromosomes 
C)  RNA (ribonucleic acid) 
D)  Nucleotides 
A)  Oncogenes 
B)  Chromosomes 
C)  RNA (ribonucleic acid) 
D)  Nucleotides

Question 11

Which of the following diagnostic procedures for analyzing DNA is the most accurate method for detecting gene rearrangements in lymphoid leukemias and lymphomas?

Accepted Answer

A)  Southern blot analysis 
B)  Northern blot analysis 
C)  Western blot analysis 
D)  Eastern blot analysis 
E)  None of the above 
A)  Southern blot analysis 
B)  Northern blot analysis 
C)  Western blot analysis 
D)  Eastern blot analysis 
E)  None of the above

Question 12

Some individuals use the term "restriction fragment length polymorphism (RFLP)" as a synonym for what method of detecting genetic alterations?

Accepted Answer

A)  Northern blot 
B)  Southern blot 
C)  Western blot 
D)  Eastern blot 
E)  None of the above 
A)  Northern blot 
B)  Southern blot 
C)  Western blot 
D)  Eastern blot 
E)  None of the above

Question 13

DNA extracted from fresh cells or frozen tissue is resuspended in buffer and can be stored at 4°C for how long prior to amplification?

Accepted Answer

A)  6 hours 
B)  24 hours 
C)  7 days 
D)  14 days 
E)  1 month 
A)  6 hours 
B)  24 hours 
C)  7 days 
D)  14 days 
E)  1 month

Question 14

Which structures serve as templates for RNA transcription and protein translation?

Accepted Answer

A)  Lipids 
B)  Amino acids 
C)  Genes 
D)  Ribonucleotides 
A)  Lipids 
B)  Amino acids 
C)  Genes 
D)  Ribonucleotides

Exam 35: Molecular Diagnostic Techniques in Hematopathology

A gene whose alteration is responsible for cancer formation is called a(n) _________.

Which of the following is/are major advantages of PCR?

DNA or RNA is readily extracted from which of the following specimens?

The restriction endonuclease HindIII is derived from which of the following organisms?

Which of the following is/are probe label(s)?

Which of the following statements describe a probe as used in molecular diagnostics?

DNA extracted from fresh cells or frozen tissue is resuspended in buffer and can be stored indefinitely at what temperature prior to amplification?

DNA and RNA are quantitated spectrophotometrically because nucleic acids absorb ultraviolet light at what optical density?

Intact DNA is usually well preserved in blood samples that are less than how many days old?

Which substance acts as an intermediary to DNA in transmitting genetic information?

Which of the following diagnostic procedures for analyzing DNA is the most accurate method for detecting gene rearrangements in lymphoid leukemias and lymphomas?

Some individuals use the term "restriction fragment length polymorphism (RFLP)" as a synonym for what method of detecting genetic alterations?

DNA extracted from fresh cells or frozen tissue is resuspended in buffer and can be stored at 4°C for how long prior to amplification?

Which structures serve as templates for RNA transcription and protein translation?

Morphology of Human Blood and Marrow Cells: Hematopoiesis

Bone Marrow

The Red Blood Cell: Structure and Function

Anemia: Diagnosis and Clinical Considerations

Evaluation of Red Blood Cell Morphology and Introduction to Platelet and White Blood Cell Morphology

Iron Metabolism and Hypochromic Anemias

Megaloblastic Anemias

Aplastic Anemia Including Pure Red Cell Aplasia and Congenital Dyserythropoietic Anemia and Paroxysmal Nocturnal Hemoglobinuria

Introduction to Hemolytic Anemias: Intracorpuscular Defects: I Hereditary Defects of the Red Cell Membrane

Hemolytic Anemias: Intracorpuscular Defects: II Hereditary Enzyme Deficiencies

Hemolytic Anemias: Intracorpuscular Defects: III the Hemoglobinopathies

Hemolytic Anemias: Intracorpuscular Defects: Iv Thalassemia

Hemolytic Anemias: Extracorpuscular Defects

Hypoproliferative Anemia: Anemia Associated With Systemic Diseases

Cell Biology, Disorders of Neutrophils, Infectious Mononucleosis, and Reactive Lymphocytosis

Introduction to Leukemia and the Acute Leukemias

Chronic Myeloproliferative Disorders I: Chronic Myelogenous Leukemia

Chronic Myeloproliferative Disorders Ii: Polycythemia Vera, Essential Thrombocythemia, and Idiopathic Myelofibrosis

Myelodysplastic Syndromes

Chronic Lymphocytic Leukemia and Related Lymphoproliferative Disorders

The Lymphomas

Multiple Myeloma and Related Plasma Cell Disorders

Lipid Lysosomal Storage Diseases and Histiocytosis

Introduction to Hemostasis

Disorders of Primary Hemostasis: Quantitative and Qualitative Platelet Disorders and Vascular Disorders

Disorders of Plasma Clotting Factors

Interaction of the Fibrinolytic, Coagulation, and Kinin Systems; Disseminated Intravascular Coagulation; and Related Pathology

Introduction to Thrombosis and Anticoagulant Therapy

Quality Control in the Hematology Laboratory

Body Fluid Examination: the Qualitative, Quantitative, and Morphologic Analysis of Serous, Cerebrospinal, and Synovial Fluids

Hematology Methods

Principles of Automated Differential Analysis

Coagulation Procedures

Applications of Flow Cytometry to Hematology and Hemostasis

Special Stainscytochemistry

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