Question 1

What is the specimen of choice for the specific esterase (naphthol AS-D chloroacetate) stain?

Accepted Answer

A)  Fresh capillary blood 
B)  Oxalated blood 
C)  Heparinized blood 
D)  Ethylenediaminetetraacetic acid-anticoagulated blood 
E)  None of the above 
A)  Fresh capillary blood 
B)  Oxalated blood 
C)  Heparinized blood 
D)  Ethylenediaminetetraacetic acid-anticoagulated blood 
E)  None of the above

Question 2

Material that stains PAS positive appears __________.

Accepted Answer

A)  Bluish-green 
B)  Bright orange 
C)  Bright red 
D)  Pink 
E)  None of the above 
A)  Bluish-green 
B)  Bright orange 
C)  Bright red 
D)  Pink 
E)  None of the above

Question 3

What is the principle of the TdT test?

Accepted Answer

A)  Washed lymphocytes are incubated with washed sheep red blood cells; rosettes will form if TdT is present. 
B)  Phycoerythrin (P
E) -labeled antigoat antibody is incubated with cells; if nuclei contain TdT, cells will appear greenish-yellow. 
C)  Fluorescein isothiocyanate (FIT
C) -labeled antirabbit antibody is incubated with cells, if cells contain TdT in nucleus they will fluoresce. 
D)  All of the above. 
E)  None of the above. 
A)  Washed lymphocytes are incubated with washed sheep red blood cells; rosettes will form if TdT is present. 
B)  Phycoerythrin (P
E) -labeled antigoat antibody is incubated with cells; if nuclei contain TdT, cells will appear greenish-yellow. 
C)  Fluorescein isothiocyanate (FIT
C) -labeled antirabbit antibody is incubated with cells, if cells contain TdT in nucleus they will fluoresce. 
D)  All of the above. 
E)  None of the above.

Question 4

Which is the anticoagulant of choice in the LAP staining procedure?

Accepted Answer

A)  Ethylenediaminetetraacetic acid (EDT
A)  
B)  Heparin 
C)  Sodium citrate 
D)  Sodium fluoride 
E)  None of the above 
A)  Ethylenediaminetetraacetic acid (EDT
A)  
B)  Heparin 
C)  Sodium citrate 
D)  Sodium fluoride 
E)  None of the above

Question 5

The alternate peroxidase stain differs from the regular procedure by using the substrate __________, which results in peroxidase positive granules stained __________.

Accepted Answer

A)  Benzidine dihydrochloride/red-brown 
B)  Benzidine dihydrochloride/black 
C)  3-Amino-9-ethylcarbazole/red-brown 
D)  3-Amino-9-ethylcarbazole/black 
E)  None of the above 
A)  Benzidine dihydrochloride/red-brown 
B)  Benzidine dihydrochloride/black 
C)  3-Amino-9-ethylcarbazole/red-brown 
D)  3-Amino-9-ethylcarbazole/black 
E)  None of the above

Question 6

PAS stains __________ in the cell.

Accepted Answer

A)  Lipid 
B)  Glycogen 
C)  Mitochondria 
D)  Proteins 
E)  None of the above 
A)  Lipid 
B)  Glycogen 
C)  Mitochondria 
D)  Proteins 
E)  None of the above

Question 7

Terminal deoxynucleotidyl transferase (TdT) is an enzyme marker for primitive __________ cells.

Accepted Answer

A)  Megakaryocytoid 
B)  Myeloid 
C)  Lymphoid 
D)  All of the above 
E)  None of the above 
A)  Megakaryocytoid 
B)  Myeloid 
C)  Lymphoid 
D)  All of the above 
E)  None of the above

Question 8

An LAP classification score of 2 corresponds to what morphology?

Accepted Answer

A)  Colorless 
B)  Diffuse, slight positivity or occasional granules 
C)  Diffuse positivity and moderate number of granules 
D)  Strong positive (granules fill cell) 
E)  Very deep staining, nucleus almost obscured 
A)  Colorless 
B)  Diffuse, slight positivity or occasional granules 
C)  Diffuse positivity and moderate number of granules 
D)  Strong positive (granules fill cell) 
E)  Very deep staining, nucleus almost obscured

Question 9

Auer rods are peroxidase __________.

Accepted Answer

A)  Positive 
B)  Negative 
C)  Variable 
D)  All of the above 
E)  None of the above 
A)  Positive 
B)  Negative 
C)  Variable 
D)  All of the above 
E)  None of the above

Question 10

In the combined esterase staining procedure, a dark, brown-red staining indicates:

Accepted Answer

A)  Monocytes 
B)  T lymphocytes 
C)  Early promyelocytes 
D)  Mast cells 
E)  Late granulocytes 
A)  Monocytes 
B)  T lymphocytes 
C)  Early promyelocytes 
D)  Mast cells 
E)  Late granulocytes

Question 11

From what population is the control specimens obtained for LAP staining?

Accepted Answer

A)  Patients with chronic myelogenous leukemia 
B)  Patients with liver disease 
C)  Patients in their third trimester of pregnancy 
D)  All of the above 
E)  None of the above 
A)  Patients with chronic myelogenous leukemia 
B)  Patients with liver disease 
C)  Patients in their third trimester of pregnancy 
D)  All of the above 
E)  None of the above

Question 12

The peroxidase reaction parallels the Sudan black B stain except that the peroxidase enzyme is found only in __________ granules.

Accepted Answer

A)  Primary 
B)  Secondary 
C)  Tertiary 
D)  All of the above 
E)  None of the above 
A)  Primary 
B)  Secondary 
C)  Tertiary 
D)  All of the above 
E)  None of the above

Question 13

This esterase staining procedure utilizes both alpha-naphthol butyrate and naphthol AS-D chloroacetate as substrates.

Accepted Answer

A)  Nonspecific esterase 
B)  Specific esterase 
C)  Combined esterase 
D)  All of the above 
E)  None of the above 
A)  Nonspecific esterase 
B)  Specific esterase 
C)  Combined esterase 
D)  All of the above 
E)  None of the above

Question 14

The acid phosphatase/tartrate resistant stain is used for making diagnosis of:

Accepted Answer

A)  Thalassemia 
B)  Hairy cell leukemia 
C)  Acute myeloblastic leukemia 
D)  Chronic myelocytic leukemia 
E)  None of the above 
A)  Thalassemia 
B)  Hairy cell leukemia 
C)  Acute myeloblastic leukemia 
D)  Chronic myelocytic leukemia 
E)  None of the above

Question 15

What is the first cell in the myeloid lineage in which the enzyme peroxidase can be detected?

Accepted Answer

A)  Metamyelocyte 
B)  Band neutrophils 
C)  Neutrophil 
D)  Promyelocyte 
E)  Myelocyte 
A)  Metamyelocyte 
B)  Band neutrophils 
C)  Neutrophil 
D)  Promyelocyte 
E)  Myelocyte

Question 16

In the Sudan black B staining procedures, the granules of myelocytic precursors stain __________.

Accepted Answer

A)  Brownish-black 
B)  Red-brown 
C)  Orange-red 
D)  Pink 
E)  None of the above 
A)  Brownish-black 
B)  Red-brown 
C)  Orange-red 
D)  Pink 
E)  None of the above

Question 17

Red-brown peroxidase positive granules are found in:

Accepted Answer

A)  Promyelocytes 
B)  Myelocytes 
C)  Metamyelocytes 
D)  All of the above 
E)  None of the above 
A)  Promyelocytes 
B)  Myelocytes 
C)  Metamyelocytes 
D)  All of the above 
E)  None of the above

Question 18

____________ percent of patients in CML blast crisis exhibit positive TdT activity.

Accepted Answer

A)  50 
B)  5 
C)  20 
D)  30 
E)  Zero 
A)  50 
B)  5 
C)  20 
D)  30 
E)  Zero

Exam 36: Special Stainscytochemistry

What is the specimen of choice for the specific esterase (naphthol AS-D chloroacetate) stain?

Material that stains PAS positive appears __________.

What is the principle of the TdT test?

Which is the anticoagulant of choice in the LAP staining procedure?

The alternate peroxidase stain differs from the regular procedure by using the substrate __________, which results in peroxidase positive granules stained __________.

PAS stains __________ in the cell.

Terminal deoxynucleotidyl transferase (TdT) is an enzyme marker for primitive __________ cells.

An LAP classification score of 2 corresponds to what morphology?

Auer rods are peroxidase __________.

In the combined esterase staining procedure, a dark, brown-red staining indicates:

From what population is the control specimens obtained for LAP staining?

The peroxidase reaction parallels the Sudan black B stain except that the peroxidase enzyme is found only in __________ granules.

This esterase staining procedure utilizes both alpha-naphthol butyrate and naphthol AS-D chloroacetate as substrates.

The acid phosphatase/tartrate resistant stain is used for making diagnosis of:

What is the first cell in the myeloid lineage in which the enzyme peroxidase can be detected?

In the Sudan black B staining procedures, the granules of myelocytic precursors stain __________.

Red-brown peroxidase positive granules are found in:

____________ percent of patients in CML blast crisis exhibit positive TdT activity.

Morphology of Human Blood and Marrow Cells: Hematopoiesis

Bone Marrow

The Red Blood Cell: Structure and Function

Anemia: Diagnosis and Clinical Considerations

Evaluation of Red Blood Cell Morphology and Introduction to Platelet and White Blood Cell Morphology

Iron Metabolism and Hypochromic Anemias

Megaloblastic Anemias

Aplastic Anemia Including Pure Red Cell Aplasia and Congenital Dyserythropoietic Anemia and Paroxysmal Nocturnal Hemoglobinuria

Introduction to Hemolytic Anemias: Intracorpuscular Defects: I Hereditary Defects of the Red Cell Membrane

Hemolytic Anemias: Intracorpuscular Defects: II Hereditary Enzyme Deficiencies

Hemolytic Anemias: Intracorpuscular Defects: III the Hemoglobinopathies

Hemolytic Anemias: Intracorpuscular Defects: Iv Thalassemia

Hemolytic Anemias: Extracorpuscular Defects

Hypoproliferative Anemia: Anemia Associated With Systemic Diseases

Cell Biology, Disorders of Neutrophils, Infectious Mononucleosis, and Reactive Lymphocytosis

Introduction to Leukemia and the Acute Leukemias

Chronic Myeloproliferative Disorders I: Chronic Myelogenous Leukemia

Chronic Myeloproliferative Disorders Ii: Polycythemia Vera, Essential Thrombocythemia, and Idiopathic Myelofibrosis

Myelodysplastic Syndromes

Chronic Lymphocytic Leukemia and Related Lymphoproliferative Disorders

The Lymphomas

Multiple Myeloma and Related Plasma Cell Disorders

Lipid Lysosomal Storage Diseases and Histiocytosis

Introduction to Hemostasis

Disorders of Primary Hemostasis: Quantitative and Qualitative Platelet Disorders and Vascular Disorders

Disorders of Plasma Clotting Factors

Interaction of the Fibrinolytic, Coagulation, and Kinin Systems; Disseminated Intravascular Coagulation; and Related Pathology

Introduction to Thrombosis and Anticoagulant Therapy

Quality Control in the Hematology Laboratory

Body Fluid Examination: the Qualitative, Quantitative, and Morphologic Analysis of Serous, Cerebrospinal, and Synovial Fluids

Hematology Methods

Principles of Automated Differential Analysis

Coagulation Procedures

Applications of Flow Cytometry to Hematology and Hemostasis

Molecular Diagnostic Techniques in Hematopathology

Filters

The alternate peroxidase stain differs from the regular procedure by using the substrate , which results in peroxidase positive granules stained .