Question 1

How may recombinant genes be introduced into grain plants?

Accepted Answer

A)  by the use of Ti vectors 
B)  by the use of plasmids as vectors 
C)  by the use of genetic shotguns 
D)  by the use of radioactive probes 
E)  by the use of bacteriophages as vectors 
A)  by the use of Ti vectors 
B)  by the use of plasmids as vectors 
C)  by the use of genetic shotguns 
D)  by the use of radioactive probes 
E)  by the use of bacteriophages as vectors

Question 2

MATCHING
Match the sequence of the steps for the Southern Blot assay.
a.visualize DNA fragments using UV light
d.place filter and radioactively labeled probe together in sealed bag
b.resulting radiograph shows hybridized DNA fragments
e.load DNA fragments on gel and separate by electrophoresis
c.blotting buffer transfers DNA fragments toDNA binding filter
-step 1

Accepted Answer

The answer of MATCHING
Match the sequence of the steps for...

Question 3

Dideoxynucleotides are used in:

Accepted Answer

A)  DNA ligation. 
B)  Southern blotting. 
C)  polymerase chain reaction. 
D)  DNA sequencing. 
E)  gel electrophoresis. 
A)  DNA ligation. 
B)  Southern blotting. 
C)  polymerase chain reaction. 
D)  DNA sequencing. 
E)  gel electrophoresis.

Question 4

The presence of detectable variation in the genomes of different individuals of a population is termed:

Accepted Answer

A)  DNA sequencing. 
B)  DNA electrophoresis. 
C)  DNA tandem repeats. 
D)  polymorphism. 
E)  DNA profiling. 
A)  DNA sequencing. 
B)  DNA electrophoresis. 
C)  DNA tandem repeats. 
D)  polymorphism. 
E)  DNA profiling.

Question 5

To produce a genomic library DNA, DNA fragments are incorporated into plasmids which are injected in bacteria.
__________________

Accepted Answer

A) True 
 B)False

Question 6

A cDNA library is complementary to DNA and does not contain introns.
__________________

Accepted Answer

A) True 
 B)False

Question 7

List and briefly explain three properties of plasmids that allow them to be used as effective DNA cloning vectors.

Accepted Answer

Plasmids have an origin of rep

Question 8

The FBI currently determines an individual's DNA profile using:

Accepted Answer

A)  DNA sequencing. 
B)  mitochondrial DNA variations. 
C)  RFLPs. 
D)  DNA ligation. 
E)  13 STR markers. 
A)  DNA sequencing. 
B)  mitochondrial DNA variations. 
C)  RFLPs. 
D)  DNA ligation. 
E)  13 STR markers.

Question 9

The use of organisms to develop useful products is called:

Accepted Answer

A)  bioengineering. 
B)  in vitro gene technology. 
C)  biotechnology. 
D)  recombinant DNA technology. 
E)  genetic engineering. 
A)  bioengineering. 
B)  in vitro gene technology. 
C)  biotechnology. 
D)  recombinant DNA technology. 
E)  genetic engineering.

Question 10

The area of genomics that is concerned with mapping and sequencing genomes is called __________ genomics.

Accepted Answer

A)  comparative 
B)  structural 
C)  RFLP 
D)  EST 
E)  functional 
A)  comparative 
B)  structural 
C)  RFLP 
D)  EST 
E)  functional

Question 11

Why is it unlikely that genetically engineered strains of bacteria would pose a threat to the environment?

Accepted Answer

A)  Genetically engineered bacteria cannot survive exposure to UV radiation. 
B)  Genetically engineered strains of bacteria are strictly regulated, making accidental release unlikely. 
C)  Genetically engineered strains of bacteria cannot survive exposure to oxygen. 
D)  Genetically engineered strains of bacteria are poor competitors of wild strains. 
E)  Genetically engineered strains of bacteria require supplemental nutrients to survive. 
A)  Genetically engineered bacteria cannot survive exposure to UV radiation. 
B)  Genetically engineered strains of bacteria are strictly regulated, making accidental release unlikely. 
C)  Genetically engineered strains of bacteria cannot survive exposure to oxygen. 
D)  Genetically engineered strains of bacteria are poor competitors of wild strains. 
E)  Genetically engineered strains of bacteria require supplemental nutrients to survive.

Question 12

Figure 15-3
Use the figure to answer the corresponding question(s).  
-The columns in Figure 15-3 represent F (for female), C (for children), and M (for male).The presence of the band marked with arrow #2, shows that:

Accepted Answer

A)  this DNA sequence in the children is a mutation. 
B)  this DNA sequence in the children is inherited from their mother. 
C)  this DNA sequence in the children is inherited from their father. 
D)  this sequence in the children is inherited from both their mother and father. 
E)  individual F is not the mother of these children. 
A)  this DNA sequence in the children is a mutation. 
B)  this DNA sequence in the children is inherited from their mother. 
C)  this DNA sequence in the children is inherited from their father. 
D)  this sequence in the children is inherited from both their mother and father. 
E)  individual F is not the mother of these children.

Question 13

The chain termination method is used in:

Accepted Answer

A)  DNA sequencing. 
B)  gel electrophoresis. 
C)  Southern blotting. 
D)  DNA ligation. 
E)  polymerase chain reaction. 
A)  DNA sequencing. 
B)  gel electrophoresis. 
C)  Southern blotting. 
D)  DNA ligation. 
E)  polymerase chain reaction.

Question 14

A __________ is required to transfer genes from one organism to another.

Accepted Answer

A)  vector 
B)  reverse transcriptase 
C)  transport molecule 
D)  genetic probe 
E)  PCR device 
A)  vector 
B)  reverse transcriptase 
C)  transport molecule 
D)  genetic probe 
E)  PCR device

Question 15

MATCHING
Match the sequence of the steps for the Southern Blot assay.
a.visualize DNA fragments using UV light
d.place filter and radioactively labeled probe together in sealed bag
b.resulting radiograph shows hybridized DNA fragments
e.load DNA fragments on gel and separate by electrophoresis
c.blotting buffer transfers DNA fragments toDNA binding filter
-step 5

Accepted Answer

The answer of MATCHING
Match the sequence of the steps for...

Question 16

After a protein coding gene is identified, its function can be studied by using RNA interference to activate the gene.

Accepted Answer

A) True 
 B)False

Question 17

Which DNA sequence below would be a palindromic sequence?

Accepted Answer

A)  AGCGCT 
B)  AGCAGC 
C)  AAAGGG 
D)  ACACAC 
E)  AGTCGT 
A)  AGCGCT 
B)  AGCAGC 
C)  AAAGGG 
D)  ACACAC 
E)  AGTCGT

Question 18

Why is human blood clotting factor VIII produced by genetically engineered bacteria better to treat hemophilia type A than the clotting factor isolated from human or animal blood?

Accepted Answer

A)  Recombinantly produced clotting factor has a longer shelf life than natural clotting factor. 
B)  Clotting factor isolated from blood can transmit infectious agents. 
C)  Clotting factor isolated from human blood can cause transfusion reactions. 
D)  Recombinantly produced clotting factor is free of blood thinning factors. 
E)  Natural clotting factor from blood is not very active. 
A)  Recombinantly produced clotting factor has a longer shelf life than natural clotting factor. 
B)  Clotting factor isolated from blood can transmit infectious agents. 
C)  Clotting factor isolated from human blood can cause transfusion reactions. 
D)  Recombinantly produced clotting factor is free of blood thinning factors. 
E)  Natural clotting factor from blood is not very active.

Question 19

What purpose do restriction enzymes play in bacterial cells?

Accepted Answer

A)  Restriction enzymes prevent the overproduction of mRNA in the bacterial cell. 
B)  Restriction enzymes attack bacteriophage DNA when it enters the cell. 
C)  Restriction enzymes promote bonding of the RNA polymerase to the promoter. 
D)  Restriction enzymes limit the rate of bacterial replication. 
E)  Restriction enzymes connect Okazaki fragments. 
A)  Restriction enzymes prevent the overproduction of mRNA in the bacterial cell. 
B)  Restriction enzymes attack bacteriophage DNA when it enters the cell. 
C)  Restriction enzymes promote bonding of the RNA polymerase to the promoter. 
D)  Restriction enzymes limit the rate of bacterial replication. 
E)  Restriction enzymes connect Okazaki fragments.

Exam 15: Dna Technology and Genomics

How may recombinant genes be introduced into grain plants?

Dideoxynucleotides are used in:

The presence of detectable variation in the genomes of different individuals of a population is termed:

To produce a genomic library DNA, DNA fragments are incorporated into plasmids which are injected in bacteria. __________________

A cDNA library is complementary to DNA and does not contain introns. __________________

List and briefly explain three properties of plasmids that allow them to be used as effective DNA cloning vectors.

The FBI currently determines an individual's DNA profile using:

The use of organisms to develop useful products is called:

The area of genomics that is concerned with mapping and sequencing genomes is called __________ genomics.

Why is it unlikely that genetically engineered strains of bacteria would pose a threat to the environment?

Figure 15-3 Use the figure to answer the corresponding question(s). -The columns in Figure 15-3 represent F (for female), C (for children), and M (for male).The presence of the band marked with arrow #2, shows that:

The chain termination method is used in:

A __________ is required to transfer genes from one organism to another.

After a protein coding gene is identified, its function can be studied by using RNA interference to activate the gene.

Which DNA sequence below would be a palindromic sequence?

Why is human blood clotting factor VIII produced by genetically engineered bacteria better to treat hemophilia type A than the clotting factor isolated from human or animal blood?

What purpose do restriction enzymes play in bacterial cells?

A View of Life

Atoms and Molecules: the Chemical Basis of Life

The Chemistry of Life: Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp: Energy-Releasing Pathways

Photosynthesis: Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

Dna: the Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity: Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure, Growth, and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function: an Introduction

Protection, Support, and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System: Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology: Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

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