Question 1

Bacteriophages are:

Accepted Answer

A)  viruses that infect bacteria. 
B)  plasmids that infect bacteria. 
C)  bacteria that infect other bacteria. 
D)  enzymes that destroy bacteria. 
E)  recombinant organisms. 
A)  viruses that infect bacteria. 
B)  plasmids that infect bacteria. 
C)  bacteria that infect other bacteria. 
D)  enzymes that destroy bacteria. 
E)  recombinant organisms.

Question 2

__________ is a technique that can be used to separate DNA molecules on the basis of their size.

Accepted Answer

A)  Separation gradient 
B)  Electrophoresis 
C)  Selective sorting 
D)  Cloning 
E)  PCR 
A)  Separation gradient 
B)  Electrophoresis 
C)  Selective sorting 
D)  Cloning 
E)  PCR

Question 3

Figure 15-1
Use the figure to answer the corresponding question(s).  
-To avoid the introduction of introns into the vector, a __________ copy of mature mRNA is made, using the enzyme __________.

Accepted Answer

A)  sDNA; RNA polymerase 
B)  cDNA; DNA ligase 
C)  cDNA; reverse transcriptase 
D)  sDNA; reverse transcriptase 
E)  cDNA; DNA polymerase 
A)  sDNA; RNA polymerase 
B)  cDNA; DNA ligase 
C)  cDNA; reverse transcriptase 
D)  sDNA; reverse transcriptase 
E)  cDNA; DNA polymerase

Question 4

When DNA ligase is used to combine a human fragment with a plasmid, the result is a redundant plasmid.
__________________

Accepted Answer

A) True 
 B)False

Question 5

Figure 15-1
Use the figure to answer the corresponding question(s).  
-What process or enzyme was used for Step 1 in Figure 15-1?

Accepted Answer

A)  PCR 
B)  a bacteriophage 
C)  a reverse transcriptase 
D)  a DNA ligase 
E)  a restriction enzyme 
A)  PCR 
B)  a bacteriophage 
C)  a reverse transcriptase 
D)  a DNA ligase 
E)  a restriction enzyme

Question 6

DNA ligase links two __________ DNA fragments by __________ bonds.

Accepted Answer

A)  complementary; hydrogen 
B)  circular; covalent 
C)  palindromic; covalent 
D)  linear; covalent 
E)  linear; hydrogen 
A)  complementary; hydrogen 
B)  circular; covalent 
C)  palindromic; covalent 
D)  linear; covalent 
E)  linear; hydrogen

Question 7

Figure 15-3
Use the figure to answer the corresponding question(s).  
-Retroviruses make __________ by the process of __________.

Accepted Answer

A)  RNA copies of DNA; reverse transcription 
B)  DNA copies of RNA; reverse transcription 
C)  genetic probes; Southern blotting 
D)  genetic probes; Northern blotting 
E)  telomerase; mutagenesis 
A)  RNA copies of DNA; reverse transcription 
B)  DNA copies of RNA; reverse transcription 
C)  genetic probes; Southern blotting 
D)  genetic probes; Northern blotting 
E)  telomerase; mutagenesis

Question 8

Which statement about cloning vectors is false?

Accepted Answer

A)  Bacteriophage cloning vectors can accommodate DNA fragments up to 23 kb. 
B)  Cloning vectors are molecules capable of transporting a DNA fragment into a cell. 
C)  Plasmid cloning vectors can accommodate DNA fragments up to 20 kb. 
D)  Bacterial artificial chromosomes can accommodate fragments up to 200 mb. 
E)  Cosmid cloning vectors combine the features of both bacteriophages and plasmids. 
A)  Bacteriophage cloning vectors can accommodate DNA fragments up to 23 kb. 
B)  Cloning vectors are molecules capable of transporting a DNA fragment into a cell. 
C)  Plasmid cloning vectors can accommodate DNA fragments up to 20 kb. 
D)  Bacterial artificial chromosomes can accommodate fragments up to 200 mb. 
E)  Cosmid cloning vectors combine the features of both bacteriophages and plasmids.

Question 9

A genomic library consists of fragments of all of the DNA:

Accepted Answer

A)  that occurs in one species. 
B)  contained in one chromosome. 
C)  that would be found in a typical cell of a particular organism. 
D)  in a recombinant gene. 
E)  that codes for functional enzymes in a typical cell of a particular organism. 
A)  that occurs in one species. 
B)  contained in one chromosome. 
C)  that would be found in a typical cell of a particular organism. 
D)  in a recombinant gene. 
E)  that codes for functional enzymes in a typical cell of a particular organism.

Question 10

Explain how DNA sequencing can be accomplished using dideoxynucleotides.

Accepted Answer

Concepts to Consider: Four reaction mixt

Question 11

Bacteria methylate their DNA in order to:

Accepted Answer

A)  prepare for DNA replication. 
B)  prepare for mitosis. 
C)  effectively infect other cells. 
D)  protect it from restriction enzymes. 
E)  prevent viral DNA from hybridizing with bacterial DNA. 
A)  prepare for DNA replication. 
B)  prepare for mitosis. 
C)  effectively infect other cells. 
D)  protect it from restriction enzymes. 
E)  prevent viral DNA from hybridizing with bacterial DNA.

Question 12

The major problem with polymerase chain reaction is:

Accepted Answer

A)  keeping the DNA strands intact. 
B)  that it works on only some DNA sequences. 
C)  that the amplified product is often degraded. 
D)  that it is too slow. 
E)  contamination. 
A)  keeping the DNA strands intact. 
B)  that it works on only some DNA sequences. 
C)  that the amplified product is often degraded. 
D)  that it is too slow. 
E)  contamination.

Question 13

Southern blotting is a process where __________ is transferred from a gel to a __________ where a probe can be applied.

Accepted Answer

A)  DNA; water solution 
B)  protein; water solution 
C)  DNA; nitrocellulose filter 
D)  RNA; nitrocellulose filter 
E)  protein; nitrocellulose filter 
A)  DNA; water solution 
B)  protein; water solution 
C)  DNA; nitrocellulose filter 
D)  RNA; nitrocellulose filter 
E)  protein; nitrocellulose filter

Question 14

Figure 15-1
Use the figure to answer the corresponding question(s).  
-Refer to Figure 15-1.The use of antibiotic medium at the end of this process:

Accepted Answer

A)  selects against plasmids containing human DNA fragments. 
B)  selects for plasmids containing particular DNA fragments. 
C)  selects for bacteria containing plasmids. 
D)  selects for bacteria lacking plasmids. 
E)  prevents contamination of the medium. 
A)  selects against plasmids containing human DNA fragments. 
B)  selects for plasmids containing particular DNA fragments. 
C)  selects for bacteria containing plasmids. 
D)  selects for bacteria lacking plasmids. 
E)  prevents contamination of the medium.

Question 15

If a(n) intron containing a human gene sequence is inserted into a prokaryote cell, that molecule will produce a functional protein product.
__________________

Accepted Answer

A) True 
 B)False

Question 16

MATCHING
Match the sequence of the steps for the Southern Blot assay.
a.visualize DNA fragments using UV light
d.place filter and radioactively labeled probe together in sealed bag
b.resulting radiograph shows hybridized DNA fragments
e.load DNA fragments on gel and separate by electrophoresis
c.blotting buffer transfers DNA fragments toDNA binding filter
-How does a cDNA library differ from a genomic library? How are cDNA libraries significant to the production of eukaryotic gene products?

Accepted Answer

Concepts to Consider: cDNA libraries con

Question 17

MATCHING
Match the sequence of the steps for the Southern Blot assay.
a.visualize DNA fragments using UV light
d.place filter and radioactively labeled probe together in sealed bag
b.resulting radiograph shows hybridized DNA fragments
e.load DNA fragments on gel and separate by electrophoresis
c.blotting buffer transfers DNA fragments toDNA binding filter
-step 3

Accepted Answer

The answer of MATCHING
Match the sequence of the steps for...

Question 18

What are transgenic organisms? Explain the rationale behind using these organisms to produce some of the proteins needed for human use, instead of relying solely on proteins produced by genetically engineered E.coli.

Accepted Answer

Concepts to Consider: Differences in pro

Question 19

Introducing a gene for a surface protein produced by a disease-causing agent into a nonpathogenic vector is a method to:

Accepted Answer

A)  produce a new pathogen. 
B)  create a new life form. 
C)  inactivate a pathogen. 
D)  activate a nonpathogenic vector. 
E)  produce a recombinant vaccine. 
A)  produce a new pathogen. 
B)  create a new life form. 
C)  inactivate a pathogen. 
D)  activate a nonpathogenic vector. 
E)  produce a recombinant vaccine.

Question 20

In the process by which cDNA is formed, reverse transcriptase is used to produce _____ from _______.

Accepted Answer

A)  double-stranded cDNA; single-stranded mRNA 
B)  double-stranded mRNA; single-stranded cDNA 
C)  single-stranded cDNA; single-stranded mRNA 
D)  single-stranded mRNA; single-stranded cDNA 
E)  double-stranded cDNA; double-stranded mRNA 
A)  double-stranded cDNA; single-stranded mRNA 
B)  double-stranded mRNA; single-stranded cDNA 
C)  single-stranded cDNA; single-stranded mRNA 
D)  single-stranded mRNA; single-stranded cDNA 
E)  double-stranded cDNA; double-stranded mRNA

Exam 15: Dna Technology and Genomics

Bacteriophages are:

__________ is a technique that can be used to separate DNA molecules on the basis of their size.

Figure 15-1 Use the figure to answer the corresponding question(s). -To avoid the introduction of introns into the vector, a __________ copy of mature mRNA is made, using the enzyme __________.

When DNA ligase is used to combine a human fragment with a plasmid, the result is a redundant plasmid. __________________

Figure 15-1 Use the figure to answer the corresponding question(s). -What process or enzyme was used for Step 1 in Figure 15-1?

DNA ligase links two __________ DNA fragments by __________ bonds.

Figure 15-3 Use the figure to answer the corresponding question(s). -Retroviruses make __________ by the process of __________.

Which statement about cloning vectors is false?

A genomic library consists of fragments of all of the DNA:

Explain how DNA sequencing can be accomplished using dideoxynucleotides.

Bacteria methylate their DNA in order to:

The major problem with polymerase chain reaction is:

Southern blotting is a process where __________ is transferred from a gel to a __________ where a probe can be applied.

Figure 15-1 Use the figure to answer the corresponding question(s). -Refer to Figure 15-1.The use of antibiotic medium at the end of this process:

If a(n) intron containing a human gene sequence is inserted into a prokaryote cell, that molecule will produce a functional protein product. __________________

What are transgenic organisms? Explain the rationale behind using these organisms to produce some of the proteins needed for human use, instead of relying solely on proteins produced by genetically engineered E.coli.

Introducing a gene for a surface protein produced by a disease-causing agent into a nonpathogenic vector is a method to:

In the process by which cDNA is formed, reverse transcriptase is used to produce _____ from _______.

A View of Life

Atoms and Molecules: the Chemical Basis of Life

The Chemistry of Life: Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp: Energy-Releasing Pathways

Photosynthesis: Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

Dna: the Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity: Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure, Growth, and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function: an Introduction

Protection, Support, and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System: Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology: Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

Filters

Figure 15-1 Use the figure to answer the corresponding question(s). -To avoid the introduction of introns into the vector, a copy of mature mRNA is made, using the enzyme .

DNA ligase links two DNA fragments by bonds.

Figure 15-3 Use the figure to answer the corresponding question(s). -Retroviruses make by the process of .

Southern blotting is a process where is transferred from a gel to a where a probe can be applied.

In the process by which cDNA is formed, reverse transcriptase is used to produce _ from ___.