Question 1

The amplification of recombinant plasmids occurs by:

Accepted Answer

A)  the growth of the plasmid DNA. 
B)  DNA replication of plasmids outside a host cell. 
C)  the process of growth and division of the host cell. 
D)  transformation of bacterial cells. 
E)  the polymerase chain reaction. 
A)  the growth of the plasmid DNA. 
B)  DNA replication of plasmids outside a host cell. 
C)  the process of growth and division of the host cell. 
D)  transformation of bacterial cells. 
E)  the polymerase chain reaction. C

Question 2

Transgenic organisms are used to aid studies of:

Accepted Answer

A)  genetic diseases. 
B)  gene expression. 
C)  immune system function. 
D)  viral diseases. 
E)  genes involved in cancer development. 
A)  genetic diseases. 
B)  gene expression. 
C)  immune system function. 
D)  viral diseases. 
E)  genes involved in cancer development. A,B,C,D,E

Question 3

______ is an example of a plant that is genetically modified to produce high quantities of beta-carotene for conversion to vitamin A.

Accepted Answer

A)  Tobacco 
B)  Corn 
C)  Rice 
D)  Apple 
E)  Watermelon 
A)  Tobacco 
B)  Corn 
C)  Rice 
D)  Apple 
E)  Watermelon C

Question 4

Define the term restriction enzyme, and give two examples of ways in which these enzymes are used in recombinant DNA technology.

Accepted Answer

Restriction enzymes are endonucleases wi

Question 5

Gene targeting is currently being used in all of the following applications except:

Accepted Answer

A)  study of immune system function. 
B)  investigations into cancer. 
C)  study of respiratory diseases. 
D)  forensic science. 
E)  the study of development of embryos. 
A)  study of immune system function. 
B)  investigations into cancer. 
C)  study of respiratory diseases. 
D)  forensic science. 
E)  the study of development of embryos.

Question 6

The ratio of different-colored fluorescent dyes in microarray can be used to infer information about:

Accepted Answer

A)  patterns of gene expression only. 
B)  drug activity only. 
C)  the identity of disease-causing genes only. 
D)  patterns of gene expression and also drug activity only. 
E)  patterns of gene expression, drug activity and the identify of disease-causing genes. 
A)  patterns of gene expression only. 
B)  drug activity only. 
C)  the identity of disease-causing genes only. 
D)  patterns of gene expression and also drug activity only. 
E)  patterns of gene expression, drug activity and the identify of disease-causing genes.

Question 7

When RNAs separated by electrophoresis are transferred to a membrane and detected using a nucleic acid probe the result is called a Western Blot.
__________________

Accepted Answer

A) True 
 B)False

Question 8

The method of choice to determine a DNA profile on DNA-containing forensic evidence that is old or has been damaged is:

Accepted Answer

A)  nuclear DNA sequencing. 
B)  mitochondrial DNA sequencing. 
C)  STR profiling. 
D)  DNA ligation. 
E)  RFLPs. 
A)  nuclear DNA sequencing. 
B)  mitochondrial DNA sequencing. 
C)  STR profiling. 
D)  DNA ligation. 
E)  RFLPs.

Question 9

The chain termination method of DNA sequencing is based on a replicating DNA strand incorporating a deoxynucleotide that stops the chain elongation.
__________________

Accepted Answer

A) True 
 B)False

Question 10

Cosmid cloning vectors accomodate much larger fragments of DNA than bacterial artificial chromosomes.
__________________

Accepted Answer

A) True 
 B)False

Question 11

cDNA sequences are expressed sequence tags (EST) that identify protein coding genes.

Accepted Answer

A) True 
 B)False

Question 12

Proteomics is made complicated by the fact that:

Accepted Answer

A)  gene therapy is in its infancy. 
B)  DNA sequencing requires powerful computers. 
C)  the response to a drug varies with the individual. 
D)  certain genes encode several different proteins. 
E)  cells of different tissues contain the same kinds of proteins. 
A)  gene therapy is in its infancy. 
B)  DNA sequencing requires powerful computers. 
C)  the response to a drug varies with the individual. 
D)  certain genes encode several different proteins. 
E)  cells of different tissues contain the same kinds of proteins.

Question 13

The polymerase chain reaction was devised by:

Accepted Answer

A)  Francis Crick. 
B)  Walter Gilbert. 
C)  E.M.Southern. 
D)  Kary Mullis. 
E)  Leland Hartwell. 
A)  Francis Crick. 
B)  Walter Gilbert. 
C)  E.M.Southern. 
D)  Kary Mullis. 
E)  Leland Hartwell.

Question 14

Splicing together DNA from 2 different organisms is called:

Accepted Answer

A)  bioengineering. 
B)  in vitro gene technology. 
C)  biotechnology. 
D)  recombinant DNA technology. 
E)  genetic engineering. 
A)  bioengineering. 
B)  in vitro gene technology. 
C)  biotechnology. 
D)  recombinant DNA technology. 
E)  genetic engineering.

Question 15

MATCHING
Match the sequence of the steps for the Southern Blot assay.
a.visualize DNA fragments using UV light
d.place filter and radioactively labeled probe together in sealed bag
b.resulting radiograph shows hybridized DNA fragments
e.load DNA fragments on gel and separate by electrophoresis
c.blotting buffer transfers DNA fragments toDNA binding filter
-step 4

Accepted Answer

The answer of MATCHING
Match the sequence of the steps for...

Question 16

The method for DNA sequencing was developed by:

Accepted Answer

A)  Jacob. 
B)  Watson. 
C)  Hershey. 
D)  Avery. 
E)  Sanger. 
A)  Jacob. 
B)  Watson. 
C)  Hershey. 
D)  Avery. 
E)  Sanger.

Question 17

During the preparation of a human genomic library, plasmids containing human DNA fragments are inserted into:

Accepted Answer

A)  compatible human cells. 
B)  an antibiotic-resistant strain of E.coli to protect the inserted fragments. 
C)  antibiotic-sensitive E.coli cells that become antibiotic-resistant if transformed. 
D)  bacteriophages. 
E)  mRNA molecules. 
A)  compatible human cells. 
B)  an antibiotic-resistant strain of E.coli to protect the inserted fragments. 
C)  antibiotic-sensitive E.coli cells that become antibiotic-resistant if transformed. 
D)  bacteriophages. 
E)  mRNA molecules.

Question 18

The modification of the DNA of an organism to produce new genes with new traits is most properly called:

Accepted Answer

A)  bioengineering. 
B)  in vitro gene technology. 
C)  biotechnology. 
D)  recombinant DNA technology. 
E)  genetic engineering. 
A)  bioengineering. 
B)  in vitro gene technology. 
C)  biotechnology. 
D)  recombinant DNA technology. 
E)  genetic engineering.

Question 19

A chromosome library is a collection of all of the DNA:

Accepted Answer

A)  that occurs in one species. 
B)  contained in one chromosome. 
C)  that would be found in the chromosomes of a typical cell of a particular organism. 
D)  in a recombinant gene. 
E)  that codes for functional enzymes in a chromosome of a particular organism. 
A)  that occurs in one species. 
B)  contained in one chromosome. 
C)  that would be found in the chromosomes of a typical cell of a particular organism. 
D)  in a recombinant gene. 
E)  that codes for functional enzymes in a chromosome of a particular organism.

Question 20

Comparative genomics deals with the function of genes and nongene sequences in genomes.

Accepted Answer

A) True 
 B)False

Exam 15: Dna Technology and Genomics

The amplification of recombinant plasmids occurs by:

Transgenic organisms are used to aid studies of:

______ is an example of a plant that is genetically modified to produce high quantities of beta-carotene for conversion to vitamin A.

Define the term restriction enzyme, and give two examples of ways in which these enzymes are used in recombinant DNA technology.

Gene targeting is currently being used in all of the following applications except:

The ratio of different-colored fluorescent dyes in microarray can be used to infer information about:

When RNAs separated by electrophoresis are transferred to a membrane and detected using a nucleic acid probe the result is called a Western Blot. __________________

The method of choice to determine a DNA profile on DNA-containing forensic evidence that is old or has been damaged is:

The chain termination method of DNA sequencing is based on a replicating DNA strand incorporating a deoxynucleotide that stops the chain elongation. __________________

Cosmid cloning vectors accomodate much larger fragments of DNA than bacterial artificial chromosomes. __________________

cDNA sequences are expressed sequence tags (EST) that identify protein coding genes.

Proteomics is made complicated by the fact that:

The polymerase chain reaction was devised by:

Splicing together DNA from 2 different organisms is called:

The method for DNA sequencing was developed by:

During the preparation of a human genomic library, plasmids containing human DNA fragments are inserted into:

The modification of the DNA of an organism to produce new genes with new traits is most properly called:

A chromosome library is a collection of all of the DNA:

Comparative genomics deals with the function of genes and nongene sequences in genomes.

A View of Life

Atoms and Molecules: the Chemical Basis of Life

The Chemistry of Life: Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp: Energy-Releasing Pathways

Photosynthesis: Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

Dna: the Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity: Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure, Growth, and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function: an Introduction

Protection, Support, and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System: Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology: Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

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