Question 1

To join a fragment of human DNA to bacterial or yeast DNA, both the human DNA and the bacterial or yeast DNA must first be treated with the same

Accepted Answer

A)  DNA gyrase. 
B)  DNA polymerase. 
C)  restriction enzyme. 
D)  DNA ligase. 
A)  DNA gyrase. 
B)  DNA polymerase. 
C)  restriction enzyme. 
D)  DNA ligase.

Question 2

Ligase enzymes are used to covalently bind together pieces of DNA.

Accepted Answer

A) True 
 B)False

Question 3

Imagine that it is 1965 and the bacterium Thermus aquaticus has not yet been discovered. As a geneticist interested in forensics applications, you are attempting to develop a gene- amplifying technique similar to PCR. a test tube, you place a DNA sample, free nucleotides, human DNA polymerase, and the appropriate primers for DNA sequence being analyzed. You first heat the sample to 90°C, then cool it to 50°C, and then reheat it to 70°C. Based on this scenario and your knowledge of how modern PCR works, the result of these procedures will be

Accepted Answer

A)  unsuccessful, because human DNA polymerase cannot recognize the primers used. 
B)  unsuccessful, because the enzyme will denature during the third step (reheating). 
C)  unsuccessful, because the enzyme will denature during the second step (cooling). 
D)  successful copying of the DNA, because it follows modern PCR procedures almost perfectly. 
E)  unsuccessful, because the enzyme will denature during the first step (heating). 
A)  unsuccessful, because human DNA polymerase cannot recognize the primers used. 
B)  unsuccessful, because the enzyme will denature during the third step (reheating). 
C)  unsuccessful, because the enzyme will denature during the second step (cooling). 
D)  successful copying of the DNA, because it follows modern PCR procedures almost perfectly. 
E)  unsuccessful, because the enzyme will denature during the first step (heating).

Question 4

An important tool of genetic engineering is DNA, which is DNA altered by the incorporation of genes from a different organism.

Accepted Answer

The answer of An important tool of genetic engineering is...

Question 5

Restriction enzymes are useful in recombinant DNA studies because they

Accepted Answer

A)  can reproduce in bacteria. 
B)  cut DNA only at specific locations. 
C)  join the cut ends of small DNA molecules. 
D)  give plasmids antibiotic properties. 
E)  can separate pieces of DNA and RNA from each other. 
A)  can reproduce in bacteria. 
B)  cut DNA only at specific locations. 
C)  join the cut ends of small DNA molecules. 
D)  give plasmids antibiotic properties. 
E)  can separate pieces of DNA and RNA from each other.

Question 6

PCR joins many small DNA molecules into a long chain.

Accepted Answer

A) True 
 B)False

Question 7

The human genome contains about _ _ genes.

Accepted Answer

A)  1,800 
B)  100 
C)  1 million 
D)  1 billion 
E)  20,000 to 25,000 
A)  1,800 
B)  100 
C)  1 million 
D)  1 billion 
E)  20,000 to 25,000

Question 8

Which of the following is NOT a natural means of DNA recombination?

Accepted Answer

A)  Viral infection 
B)  PCR 
C)  Transformation in bacteria 
D)  Sexual reproduction 
A)  Viral infection 
B)  PCR 
C)  Transformation in bacteria 
D)  Sexual reproduction

Question 9

What is an advantage to a farmer using an insect- resistant crop plant?

Accepted Answer

A)  More pesticide use and better crop yields 
B)  Less pesticide use and better crop yields 
C)  Sterile plants, with inability to form strange hybrids 
D)  More herbicide use but less pesticide use 
A)  More pesticide use and better crop yields 
B)  Less pesticide use and better crop yields 
C)  Sterile plants, with inability to form strange hybrids 
D)  More herbicide use but less pesticide use

Question 10

There are potential risks in performing recombinant DNA research on humans and plants.

Accepted Answer

A) True 
 B)False

Question 11

Sexual reproduction in humans is an example of DNA recombination.

Accepted Answer

A) True 
 B)False

Question 12

Which of the following involves the use of human genes?

Accepted Answer

A)  Herbicide- resistant corn 
B)  Insulin production by bacteria 
C)  Bt toxin production by soybeans 
D)  Genetically altered soybean oils 
E)  Banana- based vaccine against pathogenic E. coli 
A)  Herbicide- resistant corn 
B)  Insulin production by bacteria 
C)  Bt toxin production by soybeans 
D)  Genetically altered soybean oils 
E)  Banana- based vaccine against pathogenic E. coli

Question 13

Provide one example of a medical application of biotechnology.

Accepted Answer

Possible answers are genetic s

Question 14

DNA from different bacteria may be combined using all of the following EXCEPT

Accepted Answer

A)  viruses. 
B)  plasmids. 
C)  transformation. 
D)  cloning. 
A)  viruses. 
B)  plasmids. 
C)  transformation. 
D)  cloning.

Question 15

Prenatal diagnosis is available for all of the following disorders EXCEPT

Accepted Answer

A)  Down syndrome. 
B)  AIDS. 
C)  sickle- cell anemia. 
D)  cystic fibrosis. 
A)  Down syndrome. 
B)  AIDS. 
C)  sickle- cell anemia. 
D)  cystic fibrosis.

Question 16

Which of the following is the correct sequence of PCR?

Accepted Answer

A)  Heat to 70°C (to bind primers and enzymes), then cool to 50°C (to synthesize DN
A) , and then reheat to 90°C (to separate DN
A) . 
B)  Heat to 70°C (to separate DN
A) , then cool to 50°C (to bind primers and enzymes), and then reheat to 90°C (to synthesize DN
A) . 
C)  Heat to 90°C (to separate DN
A) , then cool to 50°C (to bind primers and enzymes), and then reheat to 70°C (to synthesize DN
A) . 
D)  Heat to 90°C (to bind primers and enzymes), then cool to 50°C (to separate DN
A) , and then reheat to 70°C (to synthesize DN
A) . 
A)  Heat to 70°C (to bind primers and enzymes), then cool to 50°C (to synthesize DN
A) , and then reheat to 90°C (to separate DN
A) . 
B)  Heat to 70°C (to separate DN
A) , then cool to 50°C (to bind primers and enzymes), and then reheat to 90°C (to synthesize DN
A) . 
C)  Heat to 90°C (to separate DN
A) , then cool to 50°C (to bind primers and enzymes), and then reheat to 70°C (to synthesize DN
A) . 
D)  Heat to 90°C (to bind primers and enzymes), then cool to 50°C (to separate DN
A) , and then reheat to 70°C (to synthesize DN
A) .

Question 17

PCR is essential for

Accepted Answer

A)  making many copies of a small amount of DNA. 
B)  analyzing a person's fingerprints. 
C)  cutting DNA into many small pieces. 
D)  allowing restriction enzymes to cut DNA. 
E)  creating recombinant plasmids. 
A)  making many copies of a small amount of DNA. 
B)  analyzing a person's fingerprints. 
C)  cutting DNA into many small pieces. 
D)  allowing restriction enzymes to cut DNA. 
E)  creating recombinant plasmids.

Question 18

All of the following are goals of biotechnology EXCEPT

Accepted Answer

A)  creating humans with higher intelligence levels. 
B)  generating economic benefits. 
C)  improving agriculturally important food plants. 
D)  efficiently producing biologically important molecules. 
E)  treating disease more effectively. 
A)  creating humans with higher intelligence levels. 
B)  generating economic benefits. 
C)  improving agriculturally important food plants. 
D)  efficiently producing biologically important molecules. 
E)  treating disease more effectively.

Question 19

What is the term for manipulating the molecular basis of inheritance via recombinant DNA technology?

Accepted Answer

A)  Biotechnology 
B)  Polymerase chain reaction (PCR) 
C)  DNA fingerprinting 
D)  Restriction fragment length polymorphism (RFLP) 
E)  Mendelian genetics 
A)  Biotechnology 
B)  Polymerase chain reaction (PCR) 
C)  DNA fingerprinting 
D)  Restriction fragment length polymorphism (RFLP) 
E)  Mendelian genetics

Question 20

If you cut out a gene using the restriction enzymes EcoRI and BamHI, what enzyme(s) will you need to insert the gene into another chromosome?

Accepted Answer

A)  EcoRI only 
B)  BamHI only 
C)  Both EcoRI and BamHI 
D)  Neither of these enzymes 
A)  EcoRI only 
B)  BamHI only 
C)  Both EcoRI and BamHI 
D)  Neither of these enzymes

Exam 14: Biotechnology

To join a fragment of human DNA to bacterial or yeast DNA, both the human DNA and the bacterial or yeast DNA must first be treated with the same

Ligase enzymes are used to covalently bind together pieces of DNA.

An important tool of genetic engineering is DNA, which is DNA altered by the incorporation of genes from a different organism.

Restriction enzymes are useful in recombinant DNA studies because they

PCR joins many small DNA molecules into a long chain.

The human genome contains about _ _ genes.

Which of the following is NOT a natural means of DNA recombination?

What is an advantage to a farmer using an insect- resistant crop plant?

There are potential risks in performing recombinant DNA research on humans and plants.

Sexual reproduction in humans is an example of DNA recombination.

Which of the following involves the use of human genes?

Provide one example of a medical application of biotechnology.

DNA from different bacteria may be combined using all of the following EXCEPT

Prenatal diagnosis is available for all of the following disorders EXCEPT

Which of the following is the correct sequence of PCR?

PCR is essential for

All of the following are goals of biotechnology EXCEPT

What is the term for manipulating the molecular basis of inheritance via recombinant DNA technology?

If you cut out a gene using the restriction enzymes EcoRI and BamHI, what enzyme(s) will you need to insert the gene into another chromosome?

An Introduction to Life on Earth

Atoms, Molecules, and Life

Biological Molecules

Cell Structure and Function

Cell Membrane Structure and Function

Energy Flow in the Life of a Cell

Capturing Solar Energy: Photosynthesis

Harvesting Energy: Glycolysis and Cellular Respiration

Cellular Reproduction

Meiosis: the Basis of Sexual Reproduction

Patterns of Inheritance

Dna: the Molecule of Heredity

Gene Expression and Regulation

Principles of Evolution

How Populations Evolve

The Origin of Species

The History of Life

Systematics: Seeking Order Amid Diversity

The Diversity of Prokaryotes and Viruses

The Diversity of Protists

The Diversity of Plants

The Diversity of Fungi

Animal Diversity I: Invertebrates

Animal Diversity II: Vertebrates

Animal Behavior

Population Growth and Regulation

Community Interactions

Energy Flow and Nutrient Cycling in Ecosystems

Earths Diverse Ecosystems

Conserving Earths Biodiversity

Homeostasis and the Organization of the Animal Body

Circulation

Respiration

Nutrition and Digestion

The Urinary System

Defenses Against Disease

Chemical Control of the Animal Body: the Endocrine System

The Nervous System

The Senses

Action and Support: the Muscles and Skeleton

Animal Reproduction

Animal Development

Plant Anatomy and Nutrient Transport

Plant Reproduction and Development

Plant Responses to the Environment

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