Question 1

Restriction enzymes are "restricted" in the sense that they normally work on only bacterial DNA.

Accepted Answer

A) True 
 B)False

Question 2

Modern forensic biotechnology labs use sophisticated machines to analyze , such as D16, and to determine the number of specific genetic repeats in the samples.

Accepted Answer

A)  (STRs) 
B)  PCRs 
C)  primers 
D)  AIDs 
A)  (STRs) 
B)  PCRs 
C)  primers 
D)  AIDs

Question 3

Which of the following is NOT a valid difference between traditional and modern forms of biotechnology?

Accepted Answer

A)  Traditional biotechnology can produce cloned animals with new genes. 
B)  Traditional biotechnology was based solely on selective breeding. 
C)  Modern biotechnology can isolate and manipulate individual genes. 
D)  Modern forms of biotechnology are slower than traditional forms of biotechnology. 
A)  Traditional biotechnology can produce cloned animals with new genes. 
B)  Traditional biotechnology was based solely on selective breeding. 
C)  Modern biotechnology can isolate and manipulate individual genes. 
D)  Modern forms of biotechnology are slower than traditional forms of biotechnology.

Question 4

Sequencing the genes of humans is important because

Accepted Answer

A)  it provides information on how to build lipids in the cell. 
B)  knowing the nucleotide sequence of human genes will aid in the treatment of diseases caused by them. 
C)  it will give us a better understanding of agricultural animals. 
D)  it will allow scientists to create human clones. 
A)  it provides information on how to build lipids in the cell. 
B)  knowing the nucleotide sequence of human genes will aid in the treatment of diseases caused by them. 
C)  it will give us a better understanding of agricultural animals. 
D)  it will allow scientists to create human clones.

Question 5

Which of the following techniques amplifies a specific region of DNA?

Accepted Answer

A)  PCR 
B)  Gel electrophoresis 
C)  DNA profiles 
D)  The use of DNA probes 
A)  PCR 
B)  Gel electrophoresis 
C)  DNA profiles 
D)  The use of DNA probes

Question 6

The enzymes used to cut genes in recombinant DNA research are called

Accepted Answer

A)  DNA polymerases. 
B)  RNA polymerases. 
C)  spliceosomes. 
D)  restriction enzymes. 
E)  replicases. 
A)  DNA polymerases. 
B)  RNA polymerases. 
C)  spliceosomes. 
D)  restriction enzymes. 
E)  replicases.

Question 7

Small, accessory chromosomes found in bacteria that are useful in recombinant DNA procedures are called

Accepted Answer

A)  viroids. 
B)  bacteriophages. 
C)  plasmids. 
D)  centrioles. 
A)  viroids. 
B)  bacteriophages. 
C)  plasmids. 
D)  centrioles.

Question 8

Based on this figure, how many PCR cycles are necessary to produce 64 copies of the original DNA fragment being amplified?

Accepted Answer

A)  Three 
B)  Four 
C)  Six 
D)  One 
E)  Two 
A)  Three 
B)  Four 
C)  Six 
D)  One 
E)  Two

Question 9

In recombinant DNA technology, plasmids

Accepted Answer

A)  are the sites of protein synthesis in bacteria. 
B)  are used to insert foreign DNA into bacteria. 
C)  are isolated from the blood of mammals. 
D)  show restriction enzymes where to cut bacterial DNA. 
E)  are necessary for cellular respiration in certain plants. 
A)  are the sites of protein synthesis in bacteria. 
B)  are used to insert foreign DNA into bacteria. 
C)  are isolated from the blood of mammals. 
D)  show restriction enzymes where to cut bacterial DNA. 
E)  are necessary for cellular respiration in certain plants.

Question 10

A recombinant plasmid contains only human and bacterial DNA.

Accepted Answer

A) True 
 B)False

Question 11

The illustrated probe cannot bind to the shown DNA sequence. Which DNA sequence could this probe bind to?

Accepted Answer

A)  AGAGATATCTAT 
B)  AGAGATATGATA 
C)  AGATAGATAGAT 
D)  AGATCTATCTAT 
A)  AGAGATATCTAT 
B)  AGAGATATGATA 
C)  AGATAGATAGAT 
D)  AGATCTATCTAT

Question 12

All of the following are benefits of sequencing the human genome EXCEPT

Accepted Answer

A)  the number of genetic disorders that can be diagnosed with molecular techniques has increased. 
B)  better comparisons can be made between human genetics and the genetics of other organisms. 
C)  more genes that cause disease have been discovered. 
D)  every genetic disorder is now curable or preventable. 
E)  genes were discovered whose functions were previously unknown. 
A)  the number of genetic disorders that can be diagnosed with molecular techniques has increased. 
B)  better comparisons can be made between human genetics and the genetics of other organisms. 
C)  more genes that cause disease have been discovered. 
D)  every genetic disorder is now curable or preventable. 
E)  genes were discovered whose functions were previously unknown.

Question 13

What is the advantage of using PCR over traditional bacterial cloning to generate large quantities of a specific piece of DNA?

Accepted Answer

PCR is faster and ch

Question 14

A radioactively labeled single- stranded DNA can be used to identify a specific piece of DNA in an electrophoresis gel.

Accepted Answer

The answer of A radioactively labeled single- stranded DNA can...

Question 15

Stringent safety rules are not necessary for recombinant DNA research because it is a natural process that occurs in the absence of human intervention.

Accepted Answer

A) True 
 B)False

Question 16

How many copies of a particular gene of interest are produced from a single template DNA strand after three cycles of PCR?

Accepted Answer

A)  Three 
B)  Two 
C)  Four 
D)  Five 
E)  Eight 
A)  Three 
B)  Two 
C)  Four 
D)  Five 
E)  Eight

Question 17

In nature, DNA recombination occurs but it is random and undirected.

Accepted Answer

A) True 
 B)False

Question 18

All of the following are ways in which scientists can transfect an organism EXCEPT

Accepted Answer

A)  using a "gene gun" to shoot genes into cells in culture. 
B)  infecting a host organism with a virus carrying a new gene. 
C)  spraying plants with herbicide containing new genes. 
D)  injecting DNA into an egg using a small, sharp pipette. 
A)  using a "gene gun" to shoot genes into cells in culture. 
B)  infecting a host organism with a virus carrying a new gene. 
C)  spraying plants with herbicide containing new genes. 
D)  injecting DNA into an egg using a small, sharp pipette.

Question 19

DNA moves within an electric field because it

Accepted Answer

A)  makes up chromosomes, which are slightly electromagnetic. 
B)  is positively charged. 
C)  can possess different charges (positive or negative), depending on its base sequence. 
D)  is negatively charged. 
A)  makes up chromosomes, which are slightly electromagnetic. 
B)  is positively charged. 
C)  can possess different charges (positive or negative), depending on its base sequence. 
D)  is negatively charged.

Question 20

Compare and contrast the use of a DNA profile ("DNA fingerprinting") with regular ink fingerprinting.

Accepted Answer

Both have the ability to distinguish ind

Exam 14: Biotechnology

Restriction enzymes are "restricted" in the sense that they normally work on only bacterial DNA.

Modern forensic biotechnology labs use sophisticated machines to analyze , such as D16, and to determine the number of specific genetic repeats in the samples.

Which of the following is NOT a valid difference between traditional and modern forms of biotechnology?

Sequencing the genes of humans is important because

Which of the following techniques amplifies a specific region of DNA?

The enzymes used to cut genes in recombinant DNA research are called

Small, accessory chromosomes found in bacteria that are useful in recombinant DNA procedures are called

Based on this figure, how many PCR cycles are necessary to produce 64 copies of the original DNA fragment being amplified?

In recombinant DNA technology, plasmids

A recombinant plasmid contains only human and bacterial DNA.

The illustrated probe cannot bind to the shown DNA sequence. Which DNA sequence could this probe bind to?

All of the following are benefits of sequencing the human genome EXCEPT

What is the advantage of using PCR over traditional bacterial cloning to generate large quantities of a specific piece of DNA?

A radioactively labeled single- stranded DNA can be used to identify a specific piece of DNA in an electrophoresis gel.

Stringent safety rules are not necessary for recombinant DNA research because it is a natural process that occurs in the absence of human intervention.

How many copies of a particular gene of interest are produced from a single template DNA strand after three cycles of PCR?

In nature, DNA recombination occurs but it is random and undirected.

All of the following are ways in which scientists can transfect an organism EXCEPT

DNA moves within an electric field because it

Compare and contrast the use of a DNA profile ("DNA fingerprinting") with regular ink fingerprinting.

An Introduction to Life on Earth

Atoms, Molecules, and Life

Biological Molecules

Cell Structure and Function

Cell Membrane Structure and Function

Energy Flow in the Life of a Cell

Capturing Solar Energy: Photosynthesis

Harvesting Energy: Glycolysis and Cellular Respiration

Cellular Reproduction

Meiosis: the Basis of Sexual Reproduction

Patterns of Inheritance

Dna: the Molecule of Heredity

Gene Expression and Regulation

Principles of Evolution

How Populations Evolve

The Origin of Species

The History of Life

Systematics: Seeking Order Amid Diversity

The Diversity of Prokaryotes and Viruses

The Diversity of Protists

The Diversity of Plants

The Diversity of Fungi

Animal Diversity I: Invertebrates

Animal Diversity II: Vertebrates

Animal Behavior

Population Growth and Regulation

Community Interactions

Energy Flow and Nutrient Cycling in Ecosystems

Earths Diverse Ecosystems

Conserving Earths Biodiversity

Homeostasis and the Organization of the Animal Body

Circulation

Respiration

Nutrition and Digestion

The Urinary System

Defenses Against Disease

Chemical Control of the Animal Body: the Endocrine System

The Nervous System

The Senses

Action and Support: the Muscles and Skeleton

Animal Reproduction

Animal Development

Plant Anatomy and Nutrient Transport

Plant Reproduction and Development

Plant Responses to the Environment

Filters