Question 1

Match between columns
                        Premises: Step 1

Accepted Answer

The Answer of The radiograph shows the hybridized DNA fragments. is...

Question 2

During the preparation of a human genomic library, plasmids containing human DNA fragments are inserted into:

Accepted Answer

A)  compatible human cells 
B)  an antibiotic-resistant strain of E. coli to protect the inserted fragments 
C)  antibiotic-sensitive E. coli cells that become antibiotic-resistant if transformed 
D)  bacteriophages 
E)  mRNA molecules 
A)  compatible human cells 
B)  an antibiotic-resistant strain of E. coli to protect the inserted fragments 
C)  antibiotic-sensitive E. coli cells that become antibiotic-resistant if transformed 
D)  bacteriophages 
E)  mRNA molecules

Question 3

What purpose do restriction enzymes play in bacterial cells?

Accepted Answer

A)  Restriction enzymes prevent the overproduction of mRNA in the bacterial cell. 
B)  Restriction enzymes attack bacteriophage DNA when it enters the cell. 
C)  Restriction enzymes promote bonding of the RNA polymerase to the promoter. 
D)  Restriction enzymes limit the rate of bacterial replication. 
E)  Restriction enzymes connect Okazaki fragments. 
A)  Restriction enzymes prevent the overproduction of mRNA in the bacterial cell. 
B)  Restriction enzymes attack bacteriophage DNA when it enters the cell. 
C)  Restriction enzymes promote bonding of the RNA polymerase to the promoter. 
D)  Restriction enzymes limit the rate of bacterial replication. 
E)  Restriction enzymes connect Okazaki fragments.

Question 4

When DNA ligase is used to combine a human fragment with a plasmid, the result is a redundant plasmid.

Accepted Answer

A) True 
 B)False

Question 5

In polymerase chain reaction technology, the two strands of DNA are separated by:

Accepted Answer

A)  gel electrophoresis 
B)  treatment with restriction enzymes 
C)  centrifugation 
D)  exposure to high pH 
E)  exposure to heat 
A)  gel electrophoresis 
B)  treatment with restriction enzymes 
C)  centrifugation 
D)  exposure to high pH 
E)  exposure to heat

Question 6

The chain termination method of DNA sequencing is based on a replicating DNA strand incorporating a(n) deoxynucleotide that stops the chain elongation.

Accepted Answer

A) True 
 B)False

Question 7

The total DNA in a cell is referred to as the:

Accepted Answer

A)  genome 
B)  cDNA 
C)  library 
D)  chromosomal DNA 
E)  Human Genome Project 
A)  genome 
B)  cDNA 
C)  library 
D)  chromosomal DNA 
E)  Human Genome Project

Question 8

What is the advantage of using restriction enzymes that create sticky ends versus blunt ends for creating recombinant DNA molecules?

Accepted Answer

Since sticky ends are complementary, the

Question 9

Match between columns
                        Premises: Transgenic papayas resistant to the ring spot virus saved the papaya industry in Hawai'i.

Accepted Answer

The Answer of Advantage and Disadvantage is...

Question 10

Match between columns
                        Premises: Step 2

Accepted Answer

The Answer of Visualize the DNA fragments using UV light. is...

Question 11

Gene therapy is a new technology that is used to treat genetic disease by correction of the genetic mutation.

Accepted Answer

A) True 
 B)False

Question 12

Suppose a scientist is interested in studying DNA regulatory elements. What should she prepare first?

Accepted Answer

A)  cDNA library 
B)  genomic library 
C)  transgenic mouse 
D)  Northern blot 
E)  DNA chip 
A)  cDNA library 
B)  genomic library 
C)  transgenic mouse 
D)  Northern blot 
E)  DNA chip

Question 13

The method of choice for constructing a DNA profile on DNA-containing forensic evidence that is old or has been damaged is:

Accepted Answer

A)  nuclear DNA sequencing 
B)  mitochondrial DNA sequencing 
C)  STR profiling 
D)  DNA ligation 
E)  RFLPs 
A)  nuclear DNA sequencing 
B)  mitochondrial DNA sequencing 
C)  STR profiling 
D)  DNA ligation 
E)  RFLPs

Question 14

Explain how herbicide-tolerant weeds ("superweeds") could appear in areas nearby transgenic herbicide-tolerant crops. What issues arise with this scenario?

Accepted Answer

Transgenic herbicide-tolerant crops may

Question 15

A detectable variation in the genomes of different individuals of a population is known as:

Accepted Answer

A)  DNA sequencing 
B)  DNA electrophoresis 
C)  DNA tandem repeats 
D)  a polymorphism 
E)  DNA profiling 
A)  DNA sequencing 
B)  DNA electrophoresis 
C)  DNA tandem repeats 
D)  a polymorphism 
E)  DNA profiling

Question 16

The chain termination method of DNA sequencing was developed by:

Accepted Answer

A)  Jacob 
B)  Watson 
C)  Hershey 
D)  Avery 
E)  Sanger 
A)  Jacob 
B)  Watson 
C)  Hershey 
D)  Avery 
E)  Sanger

Question 17

The ratio of different-colored fluorescent dyes in microarray can be used to infer information about:

Accepted Answer

A)  patterns of gene expression only 
B)  drug activity only 
C)  the identity of disease-causing genes only 
D)  patterns of gene expression and also drug activity only 
E)  patterns of gene expression, drug activity, and the identity of disease-causing genes 
A)  patterns of gene expression only 
B)  drug activity only 
C)  the identity of disease-causing genes only 
D)  patterns of gene expression and also drug activity only 
E)  patterns of gene expression, drug activity, and the identity of disease-causing genes

Question 18

A ____ is required to transfer genes from one organism to another.

Accepted Answer

A)  vector 
B)  reverse transcriptase 
C)  transport molecule 
D)  genetic probe 
E)  PCR device 
A)  vector 
B)  reverse transcriptase 
C)  transport molecule 
D)  genetic probe 
E)  PCR device

Exam 15: DNA Technology and Genomics

During the preparation of a human genomic library, plasmids containing human DNA fragments are inserted into:

What purpose do restriction enzymes play in bacterial cells?

When DNA ligase is used to combine a human fragment with a plasmid, the result is a redundant plasmid.

In polymerase chain reaction technology, the two strands of DNA are separated by:

The chain termination method of DNA sequencing is based on a replicating DNA strand incorporating a(n) deoxynucleotide that stops the chain elongation.

The total DNA in a cell is referred to as the:

What is the advantage of using restriction enzymes that create sticky ends versus blunt ends for creating recombinant DNA molecules?

Gene therapy is a new technology that is used to treat genetic disease by correction of the genetic mutation.

Suppose a scientist is interested in studying DNA regulatory elements. What should she prepare first?

The method of choice for constructing a DNA profile on DNA-containing forensic evidence that is old or has been damaged is:

Explain how herbicide-tolerant weeds ("superweeds") could appear in areas nearby transgenic herbicide-tolerant crops. What issues arise with this scenario?

A detectable variation in the genomes of different individuals of a population is known as:

The chain termination method of DNA sequencing was developed by:

The ratio of different-colored fluorescent dyes in microarray can be used to infer information about:

A ____ is required to transfer genes from one organism to another.

A View of Life

Atoms and Molecules: the Chemical Basis of Life

The Chemistry of Life: Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp: Energy-Releasing Pathways

Photosynthesis: Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

DNA: The Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity: Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure, Growth, and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function: an Introduction

Protection, Support, and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System: Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology: Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

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