Question 1

Match between columns
                        Premises: All transgenic salmon are sterile females; therefore, they cannot breed with wild salmon populations.

Accepted Answer

The Answer of Disadvantage and Advantage is...

Question 2

Gene targeting:

Accepted Answer

A)  usually results in mice homozygous for the knock-out gene in every cell 
B)  is used to study orthologous genes 
C)  measures gene expression activity 
D)  is a rapid process 
E)  is a technique typically performed in humans 
A)  usually results in mice homozygous for the knock-out gene in every cell 
B)  is used to study orthologous genes 
C)  measures gene expression activity 
D)  is a rapid process 
E)  is a technique typically performed in humans

Question 3

An organism in which foreign genes have been incorporated is called a:

Accepted Answer

A)  recombinant organism 
B)  transgene 
C)  polymorphism 
D)  transgenic organism 
E)  recombinant 
A)  recombinant organism 
B)  transgene 
C)  polymorphism 
D)  transgenic organism 
E)  recombinant

Question 4

Two common methods for obtaining large quantities of a DNA fragment or gene of interest is by PCR or by DNA cloning (e.g., construction of a DNA library). Explain one advantage of using PCR and one advantage of using DNA cloning in a living cell.

Accepted Answer

Advantages of PCR: 
1. Faster (lots of D

Question 5

Identify two important proteins that are produced using genetic engineering techniques. Briefly discuss why the genetic engineering techniques used are preferred over the other methods of producing each of the proteins that you identified.

Accepted Answer

Here are three possible answers: 
1. Ins

Question 6

Match between columns
                        Premises: Transgenic pest-resistant crops may affect nontarget pests.

Accepted Answer

The Answer of Advantage and Disadvantage is...

Question 7

Why is insulin produced by genetically engineered E. coli cells superior to insulin obtained from animal sources?

Accepted Answer

A)  Animal insulin has a shorter life span than insulin produced using recombinant DNA techniques. 
B)  Recombinantly produced insulin contains human rather than animal sequences, reducing the chances of an allergic response. 
C)  Animal insulin is more difficult to purify than recombinantly produced insulin. 
D)  Recombinantly produced insulin has a much higher activity level. 
E)  Recombinantly produced insulin is easier to obtain than insulin from animals. 
A)  Animal insulin has a shorter life span than insulin produced using recombinant DNA techniques. 
B)  Recombinantly produced insulin contains human rather than animal sequences, reducing the chances of an allergic response. 
C)  Animal insulin is more difficult to purify than recombinantly produced insulin. 
D)  Recombinantly produced insulin has a much higher activity level. 
E)  Recombinantly produced insulin is easier to obtain than insulin from animals.

Question 8

Match between columns
                        Premises: Transgenic animals, such as sheep and cows, produce medicinal proteins in their milk. This creates a larger and cheaper supply of some human drugs.

Accepted Answer

The Answer of Disadvantage and Advantage is...

Question 9

Match between columns
                        Premises: gene expression; single mRNA transcripts

Accepted Answer

The Answer of DNA chip and Southern blot and bioinformatics is...

Question 10

In producing a genomic library, human DNA and plasmid DNA must first be treated with:

Accepted Answer

A)  the same restriction enzyme 
B)  different restriction enzymes 
C)  the same DNA ligase 
D)  different DNA ligases 
E)  cosmid cloning vectors 
A)  the same restriction enzyme 
B)  different restriction enzymes 
C)  the same DNA ligase 
D)  different DNA ligases 
E)  cosmid cloning vectors

Question 11

A plant virus is destroying corn crops. Scientists have determined a mutation that prevents the virus from attaching to the plant cells (and thus preventing infection) without altering the function of the protein or the corn. How would a CRISPR-based gene drive allow scientists to insert this gene into the yet-to-be-planted corn crops.

Accepted Answer

Concept to consider: A gene drive locus

Question 12

Reverse transcriptase catalyzes the production of _____ from ____.

Accepted Answer

A)  double-stranded cDNA; single-stranded mRNA 
B)  double-stranded mRNA; single-stranded cDNA 
C)  single-stranded cDNA; single-stranded mRNA 
D)  single-stranded mRNA; single-stranded cDNA 
E)  double-stranded cDNA; double-stranded mRNA 
A)  double-stranded cDNA; single-stranded mRNA 
B)  double-stranded mRNA; single-stranded cDNA 
C)  single-stranded cDNA; single-stranded mRNA 
D)  single-stranded mRNA; single-stranded cDNA 
E)  double-stranded cDNA; double-stranded mRNA

Question 13

Match between columns
                        Premises: Step 5

Accepted Answer

The Answer of Visualize the DNA fragments using UV light. is...

Question 14

Splicing together DNA from two different organisms is known as:

Accepted Answer

A)  bioengineering 
B)  in vitro gene technology 
C)  genome technology 
D)  recombinant DNA technology 
E)  DNA sequencing 
A)  bioengineering 
B)  in vitro gene technology 
C)  genome technology 
D)  recombinant DNA technology 
E)  DNA sequencing

Question 15

Figure 15-3
 
The columns in the accompanying figure represent F (for female), C (for children), and M (for male). The presence of the band marked with arrow #2, shows that:

Accepted Answer

A)  this DNA sequence in the children is a mutation. 
B)  this DNA sequence in the children is inherited from their mother. 
C)  this DNA sequence in the children is inherited from their father. 
D)  this sequence in the children is inherited from both their mother and father. 
E)  individual F is not the mother of these children. 
A)  this DNA sequence in the children is a mutation. 
B)  this DNA sequence in the children is inherited from their mother. 
C)  this DNA sequence in the children is inherited from their father. 
D)  this sequence in the children is inherited from both their mother and father. 
E)  individual F is not the mother of these children.

Question 16

Figure 15-1
 
Refer to the accompanying figure. The use of antibiotic medium at the end of this process:

Accepted Answer

A)  selects against plasmids containing human DNA fragments 
B)  selects for plasmids containing particular DNA fragments 
C)  selects for bacteria containing plasmids 
D)  selects for bacteria lacking plasmids 
E)  prevents contamination of the medium 
A)  selects against plasmids containing human DNA fragments 
B)  selects for plasmids containing particular DNA fragments 
C)  selects for bacteria containing plasmids 
D)  selects for bacteria lacking plasmids 
E)  prevents contamination of the medium

Question 17

Each individual bacterial colony contains ____ recombinant plasmid as/than the other colonies on the plate, and the DNA sequence of the recombinant plasmid present in any given colony can be identified using a ____.

Accepted Answer

A)  a different; DNA chip 
B)  the same; DNA chip 
C)  the same; DNA probe 
D)  a different; Western blot 
E)  a different; DNA probe 
A)  a different; DNA chip 
B)  the same; DNA chip 
C)  the same; DNA probe 
D)  a different; Western blot 
E)  a different; DNA probe

Question 18

DNA ligase links two ____ DNA fragments by ____ bonds.

Accepted Answer

A)  complementary; hydrogen 
B)  circular; covalent 
C)  palindromic; covalent 
D)  linear; covalent 
E)  linear; hydrogen 
A)  complementary; hydrogen 
B)  circular; covalent 
C)  palindromic; covalent 
D)  linear; covalent 
E)  linear; hydrogen

Question 19

A(n) ____ is produced by introducing a gene for a viral surface protein into a nonpathogenic vector.

Accepted Answer

A)  new pathogen 
B)  new life form 
C)  inactivated pathogen 
D)  nonpathogenic vector 
E)  recombinant vaccine 
A)  new pathogen 
B)  new life form 
C)  inactivated pathogen 
D)  nonpathogenic vector 
E)  recombinant vaccine

Question 20

To produce a genomic library DNA, DNA fragments are incorporated into plasmids that are introduced into bacteria.

Accepted Answer

A) True 
 B)False

Exam 15: DNA Technology and Genomics

Gene targeting:

An organism in which foreign genes have been incorporated is called a:

Two common methods for obtaining large quantities of a DNA fragment or gene of interest is by PCR or by DNA cloning (e.g., construction of a DNA library). Explain one advantage of using PCR and one advantage of using DNA cloning in a living cell.

Identify two important proteins that are produced using genetic engineering techniques. Briefly discuss why the genetic engineering techniques used are preferred over the other methods of producing each of the proteins that you identified.

Why is insulin produced by genetically engineered E. coli cells superior to insulin obtained from animal sources?

In producing a genomic library, human DNA and plasmid DNA must first be treated with:

Reverse transcriptase catalyzes the production of _____ from ____.

Splicing together DNA from two different organisms is known as:

Figure 15-3 The columns in the accompanying figure represent F (for female), C (for children), and M (for male). The presence of the band marked with arrow #2, shows that:

Figure 15-1 Refer to the accompanying figure. The use of antibiotic medium at the end of this process:

Each individual bacterial colony contains ____ recombinant plasmid as/than the other colonies on the plate, and the DNA sequence of the recombinant plasmid present in any given colony can be identified using a ____.

DNA ligase links two ____ DNA fragments by ____ bonds.

A(n) ____ is produced by introducing a gene for a viral surface protein into a nonpathogenic vector.

To produce a genomic library DNA, DNA fragments are incorporated into plasmids that are introduced into bacteria.

A View of Life

Atoms and Molecules: the Chemical Basis of Life

The Chemistry of Life: Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp: Energy-Releasing Pathways

Photosynthesis: Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

DNA: The Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity: Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure, Growth, and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function: an Introduction

Protection, Support, and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System: Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology: Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

Filters

Reverse transcriptase catalyzes the production of _ from .

Each individual bacterial colony contains recombinant plasmid as/than the other colonies on the plate, and the DNA sequence of the recombinant plasmid present in any given colony can be identified using a .

DNA ligase links two DNA fragments by bonds.