Question 1

Transformation is a process whereby:

Accepted Answer

A)  bacteria are transferred into plasmid cells. 
B)  viruses are transferred into bacterial cells. 
C)  plasmids are transferred into bacterial cells. 
D)  bacteria are transferred into viral cells. 
E)  plasmids are transferred into viral cells. 
A)  bacteria are transferred into plasmid cells. 
B)  viruses are transferred into bacterial cells. 
C)  plasmids are transferred into bacterial cells. 
D)  bacteria are transferred into viral cells. 
E)  plasmids are transferred into viral cells.

Question 2

A genomic library is a collection of:

Accepted Answer

A)  plasmid fragments that are spliced into genomic DNA 
B)  genomic DNA fragments that are spliced into plasmids 
C)  intron fragments that are spliced into plasmids 
D)  exon fragments that are spliced into bacterial cells 
E)  gene fragments that are spliced into main bacterial chromosome 
A)  plasmid fragments that are spliced into genomic DNA 
B)  genomic DNA fragments that are spliced into plasmids 
C)  intron fragments that are spliced into plasmids 
D)  exon fragments that are spliced into bacterial cells 
E)  gene fragments that are spliced into main bacterial chromosome

Question 3

Match between columns
                        Premises: compare DNA or protein sequences

Accepted Answer

The Answer of Southern blot and Western blot and DNA is...

Question 4

Retroviruses make ____ by the process of ____.

Accepted Answer

A)  RNA copies of DNA; reverse transcription 
B)  DNA copies of RNA; reverse transcription 
C)  genetic probes; Southern blotting 
D)  genetic probes; Northern blotting 
E)  telomerase; mutagenesis 
A)  RNA copies of DNA; reverse transcription 
B)  DNA copies of RNA; reverse transcription 
C)  genetic probes; Southern blotting 
D)  genetic probes; Northern blotting 
E)  telomerase; mutagenesis

Question 5

Transgenic salmon are genetically engineered to grow faster than their wild-type counterparts. They do not grow larger; they simply reach their full size in shorter time. Transgenic salmon that are grown in outdoor pens are all sterile (nonreproducing) females. Why is this important?

Accepted Answer

If the transgenic fish escape from their

Question 6

GTGTGTGTGT is an example of a(n) single nucleotide polymorphism.

Accepted Answer

A) True 
 B)False

Question 7

Comparative genomics is the field of genomics that studies the functions of genes and nongene sequences in genomes.

Accepted Answer

A) True 
 B)False

Question 8

____ is a technique that can be used to separate DNA molecules based on their size.

Accepted Answer

A)  Separation gradient 
B)  Electrophoresis 
C)  Selective sorting 
D)  Cloning 
E)  PCR 
A)  Separation gradient 
B)  Electrophoresis 
C)  Selective sorting 
D)  Cloning 
E)  PCR

Question 9

The FBI currently determines an individual's DNA profile using:

Accepted Answer

A)  DNA sequencing 
B)  mitochondrial DNA variations 
C)  RFLPs 
D)  DNA ligation 
E)  13 STR markers 
A)  DNA sequencing 
B)  mitochondrial DNA variations 
C)  RFLPs 
D)  DNA ligation 
E)  13 STR markers

Question 10

A chromosome library is a collection of all DNA:

Accepted Answer

A)  that occurs in one species 
B)  contained in one chromosome 
C)  that would be found in the chromosomes of a typical cell of a particular organism 
D)  in a recombinant gene 
E)  that codes for functional enzymes in a chromosome of a particular organism 
A)  that occurs in one species 
B)  contained in one chromosome 
C)  that would be found in the chromosomes of a typical cell of a particular organism 
D)  in a recombinant gene 
E)  that codes for functional enzymes in a chromosome of a particular organism

Question 11

Explain how the use of Bt crops can result in the evolution of pesticide-resistant insect populations. What issues may arise in this scenario?

Accepted Answer

As pests (insects) feed on Bt corn, the

Question 12

"Sticky ends" are:

Accepted Answer

A)  the single-stranded ends of a DNA segment created by some restriction enzymes 
B)  a problem in recombinant DNA technology because they form loops of single-stranded DNA 
C)  double-stranded ends of a DNA segment created by some restriction enzymes 
D)  sites of the origin of replication in prokaryotes 
E)  sugar molecules that are bound to the ends of a DNA fragment 
A)  the single-stranded ends of a DNA segment created by some restriction enzymes 
B)  a problem in recombinant DNA technology because they form loops of single-stranded DNA 
C)  double-stranded ends of a DNA segment created by some restriction enzymes 
D)  sites of the origin of replication in prokaryotes 
E)  sugar molecules that are bound to the ends of a DNA fragment

Question 13

Figure 15-1
 
What process or enzyme was used for Step 1 in the accompanying figure?

Accepted Answer

A)  PCR 
B)  a bacteriophage 
C)  a reverse transcriptase 
D)  a DNA ligase 
E)  a restriction enzyme 
A)  PCR 
B)  a bacteriophage 
C)  a reverse transcriptase 
D)  a DNA ligase 
E)  a restriction enzyme

Question 14

Which DNA sequence below would be a palindromic sequence?

Accepted Answer

A)  AGCGCT 
B)  AGCAGC 
C)  AAAGGG 
D)  ACACAC 
E)  AGTCGT 
A)  AGCGCT 
B)  AGCAGC 
C)  AAAGGG 
D)  ACACAC 
E)  AGTCGT

Question 15

After a protein-coding gene is identified, its function can be studied by using RNA interference to activate the gene.

Accepted Answer

A) True 
 B)False

Question 16

The purpose of the ENCODE study is to identify the functional regions of the human genome.

Accepted Answer

A) True 
 B)False

Question 17

Clues to the structure and function of a gene can be gained through bioinformatics.

Accepted Answer

A) True 
 B)False

Question 18

In the construction of a DNA library, bacterial colonies that grow on agar plates containing antibiotic can be described as:

Accepted Answer

A)  arising from one individual bacterium that contained a recombinant plasmid 
B)  arising from many individual bacteria, only some of which contain a recombinant plasmid 
C)  arising from one individual bacterium that does not contain a recombinant plasmid 
D)  arising from wild-type bacteria 
E)  containing a single bacterium, which contains a recombinant plasmid 
A)  arising from one individual bacterium that contained a recombinant plasmid 
B)  arising from many individual bacteria, only some of which contain a recombinant plasmid 
C)  arising from one individual bacterium that does not contain a recombinant plasmid 
D)  arising from wild-type bacteria 
E)  containing a single bacterium, which contains a recombinant plasmid

Question 19

Explain how DNA sequencing can be accomplished using dideoxynucleotides.

Accepted Answer

Four reaction mixtures must be prepared,

Question 20

To avoid the introduction of introns into the vector, a ____ copy of mature mRNA is made, using the enzyme ____.

Accepted Answer

A)  sDNA; RNA polymerase 
B)  cDNA; DNA ligase 
C)  cDNA; reverse transcriptase 
D)  sDNA; reverse transcriptase 
E)  cDNA; DNA polymerase 
A)  sDNA; RNA polymerase 
B)  cDNA; DNA ligase 
C)  cDNA; reverse transcriptase 
D)  sDNA; reverse transcriptase 
E)  cDNA; DNA polymerase

Exam 15: DNA Technology and Genomics

Transformation is a process whereby:

A genomic library is a collection of:

Retroviruses make ____ by the process of ____.

Transgenic salmon are genetically engineered to grow faster than their wild-type counterparts. They do not grow larger; they simply reach their full size in shorter time. Transgenic salmon that are grown in outdoor pens are all sterile (nonreproducing) females. Why is this important?

GTGTGTGTGT is an example of a(n) single nucleotide polymorphism.

Comparative genomics is the field of genomics that studies the functions of genes and nongene sequences in genomes.

____ is a technique that can be used to separate DNA molecules based on their size.

The FBI currently determines an individual's DNA profile using:

A chromosome library is a collection of all DNA:

Explain how the use of Bt crops can result in the evolution of pesticide-resistant insect populations. What issues may arise in this scenario?

"Sticky ends" are:

Figure 15-1 What process or enzyme was used for Step 1 in the accompanying figure?

Which DNA sequence below would be a palindromic sequence?

After a protein-coding gene is identified, its function can be studied by using RNA interference to activate the gene.

The purpose of the ENCODE study is to identify the functional regions of the human genome.

Clues to the structure and function of a gene can be gained through bioinformatics.

In the construction of a DNA library, bacterial colonies that grow on agar plates containing antibiotic can be described as:

Explain how DNA sequencing can be accomplished using dideoxynucleotides.

To avoid the introduction of introns into the vector, a ____ copy of mature mRNA is made, using the enzyme ____.

A View of Life

Atoms and Molecules: the Chemical Basis of Life

The Chemistry of Life: Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp: Energy-Releasing Pathways

Photosynthesis: Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

DNA: The Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity: Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure, Growth, and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function: an Introduction

Protection, Support, and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System: Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology: Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

Filters

Retroviruses make by the process of .

To avoid the introduction of introns into the vector, a copy of mature mRNA is made, using the enzyme .