Question 1

The amplification of recombinant plasmids occurs by:

Accepted Answer

A)  the growth of the plasmid DNA 
B)  DNA replication of plasmids outside a host cell 
C)  the process of growth and division of the host cell 
D)  transformation of bacterial cells 
E)  the polymerase chain reaction 
A)  the growth of the plasmid DNA 
B)  DNA replication of plasmids outside a host cell 
C)  the process of growth and division of the host cell 
D)  transformation of bacterial cells 
E)  the polymerase chain reaction C

Question 2

If a protein-coding gene is identified, its function can be studied by using RNA interference to ____.

Accepted Answer

A)  produce an RFLP 
B)  determine the DNA sequence. 
C)  turn the gene on 
D)  turn the gene off 
E)  induce a mutation 
A)  produce an RFLP 
B)  determine the DNA sequence. 
C)  turn the gene on 
D)  turn the gene off 
E)  induce a mutation D

Question 3

Single nucleotide polymorphisms are defined as junk DNA.

Accepted Answer

A) True 
 B)False  False

Question 4

When RNAs separated by electrophoresis are transferred to a membrane and detected using a nucleic acid probe, the result is called a Western blot.

Accepted Answer

A) True 
 B)False

Question 5

Match between columns
                        Premises: Farmers who plant transgenic herbicide-tolerant crops tend to use excess herbicides.

Accepted Answer

The Answer of Advantage and Disadvantage is...

Question 6

Match between columns
                        Premises: Transgenic lab animals, such as mice, are engineered to carry human genes, allowing for better study of human diseases.

Accepted Answer

The Answer of Disadvantage and Advantage is...

Question 7

Why is human blood clotting factor VIII produced by genetically engineered bacteria better to treat hemophilia type A than the clotting factor isolated from human or animal blood?

Accepted Answer

A)  Recombinantly produced clotting factor has a longer shelf life than natural clotting factor. 
B)  Clotting factor isolated from blood can transmit infectious agents present in the donated blood. 
C)  Clotting factor isolated from human blood is quickly degraded. 
D)  Recombinantly produced clotting factor is stable at high temperatures. 
E)  Clotting factor from blood has a lower activity than recombinant clotting factor. 
A)  Recombinantly produced clotting factor has a longer shelf life than natural clotting factor. 
B)  Clotting factor isolated from blood can transmit infectious agents present in the donated blood. 
C)  Clotting factor isolated from human blood is quickly degraded. 
D)  Recombinantly produced clotting factor is stable at high temperatures. 
E)  Clotting factor from blood has a lower activity than recombinant clotting factor.

Question 8

Bacteriophages are:

Accepted Answer

A)  viruses that infect bacteria 
B)  plasmids that infect bacteria 
C)  bacteria that infect other bacteria 
D)  enzymes that destroy bacteria 
E)  recombinant organisms 
A)  viruses that infect bacteria 
B)  plasmids that infect bacteria 
C)  bacteria that infect other bacteria 
D)  enzymes that destroy bacteria 
E)  recombinant organisms

Question 9

Transgenic organisms are used to aid studies of:

Accepted Answer

A)  genetic diseases. 
B)  gene expression. 
C)  immune system function. 
D)  viral diseases. 
E)  genes involved in cancer development. 
A)  genetic diseases. 
B)  gene expression. 
C)  immune system function. 
D)  viral diseases. 
E)  genes involved in cancer development.

Question 10

Match between columns
                        Premises: Gene flow from transgenic herbicide-tolerant plants may create herbicide-tolerant weeds.

Accepted Answer

The Answer of Disadvantage and Advantage is...

Question 11

If a human gene containing introns is inserted into a prokaryote cell, a functional human protein will be produced.

Accepted Answer

A) True 
 B)False

Question 12

What is one feature of "engineered" plasmids that is helpful in the isolation and analysis of cloned DNA?

Accepted Answer

A)  They can only handle DNA fragments of up to 10 kb. 
B)  They are an integral part of all eukaryotic cells. 
C)  They contain no genetic material of their own so that the cloned fragment is truly isolated. 
D)  The presence of genes that allow transformed cells to survive on different media. 
E)  The base sequence of one strand reads the same as its complement. 
A)  They can only handle DNA fragments of up to 10 kb. 
B)  They are an integral part of all eukaryotic cells. 
C)  They contain no genetic material of their own so that the cloned fragment is truly isolated. 
D)  The presence of genes that allow transformed cells to survive on different media. 
E)  The base sequence of one strand reads the same as its complement.

Question 13

Southern blotting is a process where ____ is transferred from a gel to a ____where a probe can be applied.

Accepted Answer

A)  DNA; water solution 
B)  protein; water solution 
C)  DNA; nitrocellulose filter 
D)  RNA; nitrocellulose filter 
E)  protein; nitrocellulose filter 
A)  DNA; water solution 
B)  protein; water solution 
C)  DNA; nitrocellulose filter 
D)  RNA; nitrocellulose filter 
E)  protein; nitrocellulose filter

Question 14

Why would a scientist use targeted gene silencing?

Accepted Answer

Targeted gene silencing preven

Question 15

Describe what a Genome-Wide Association Study (GWAS) examines the genome to find.

Accepted Answer

A GWAS study investigates the

Question 16

Match between columns
                        Premises: gene expression; protein products

Accepted Answer

The Answer of DNA chip and bioinformatics and Northern blot is...

Question 17

The use of organisms to develop useful products is called:

Accepted Answer

A)  bioengineering 
B)  in vitro gene technology 
C)  biotechnology 
D)  recombinant DNA technology 
E)  genetic engineering 
A)  bioengineering 
B)  in vitro gene technology 
C)  biotechnology 
D)  recombinant DNA technology 
E)  genetic engineering

Question 18

Match between columns
                        Premises: genome-wide gene expression

Accepted Answer

The Answer of bioinformatics and Southern blot and Western blot is...

Question 19

Bacteria methylate their DNA to:

Accepted Answer

A)  prepare for DNA replication 
B)  prepare for mitosis 
C)  effectively infect other cells 
D)  protect it from restriction enzymes 
E)  prevent viral DNA from hybridizing with bacterial DNA 
A)  prepare for DNA replication 
B)  prepare for mitosis 
C)  effectively infect other cells 
D)  protect it from restriction enzymes 
E)  prevent viral DNA from hybridizing with bacterial DNA

Question 20

Match between columns
                        Premises: Growing transgenic pest-resistant crops means less pesticide spraying.

Accepted Answer

The Answer of Disadvantage and Advantage is...

Exam 15: DNA Technology and Genomics

The amplification of recombinant plasmids occurs by:

If a protein-coding gene is identified, its function can be studied by using RNA interference to ____.

Single nucleotide polymorphisms are defined as junk DNA.

When RNAs separated by electrophoresis are transferred to a membrane and detected using a nucleic acid probe, the result is called a Western blot.

Why is human blood clotting factor VIII produced by genetically engineered bacteria better to treat hemophilia type A than the clotting factor isolated from human or animal blood?

Bacteriophages are:

Transgenic organisms are used to aid studies of:

If a human gene containing introns is inserted into a prokaryote cell, a functional human protein will be produced.

What is one feature of "engineered" plasmids that is helpful in the isolation and analysis of cloned DNA?

Southern blotting is a process where ____ is transferred from a gel to a ____where a probe can be applied.

Why would a scientist use targeted gene silencing?

Describe what a Genome-Wide Association Study (GWAS) examines the genome to find.

The use of organisms to develop useful products is called:

Bacteria methylate their DNA to:

A View of Life

Atoms and Molecules: the Chemical Basis of Life

The Chemistry of Life: Organic Compounds

Organization of the Cell

Biological Membranes

Cell Communication

Energy and Metabolism

How Cells Make Atp: Energy-Releasing Pathways

Photosynthesis: Capturing Light Energy

Chromosomes, Mitosis, and Meiosis

The Basic Principles of Heredity

DNA: The Carrier of Genetic Information

Gene Expression

Gene Regulation

Human Genetics and the Human Genome

Developmental Genetics

Introduction to Darwinian Evolution

Evolutionary Change in Populations

Speciation and Macroevolution

The Origin and Evolutionary History of Life

The Evolution of Primates

Understanding Diversity: Systematics

Viruses and Subviral Agents

Bacteria and Archaea

Protists

Seedless Plants

Seed Plants

The Fungi

An Introduction to Animal Diversity

Sponges, Cnidarians, Ctenophores, and Protostomes

The Deuterostomes

Plant Structure, Growth, and Development

Leaf Structure and Function

Stem Structure and Transport

Roots and Mineral Nutrition

Reproduction in Flowering Plants

Plant Developmental Responses to External and Internal Signals

Animal Structure and Function: an Introduction

Protection, Support, and Movement

Neural Signaling

Neural Regulation

Sensory Systems

Internal Transport

The Immune System: Internal Defense

Gas Exchange

Processing Food and Nutrition

Osmoregulation and Disposal of Metabolic Wastes

Endocrine Regulation

Reproduction

Animal Development

Animal Behavior

Introduction to Ecology: Population Ecology

Community Ecology

Ecosystems and the Biosphere

Ecology and the Geography of Life

Biological Diversity and Conservation Biology

Filters

Southern blotting is a process where is transferred from a gel to a where a probe can be applied.