Question 1

What red cell morphology would support the red cell indices calculated for Mary Jones in Question 14?

Accepted Answer

A)  Microcytic hyperchromic red cells 
B)  Normocytic hyperchromic red cells 
C)  Macrocytic hyperchromic red cells 
D)  No red cell morphology supports these indices. 
A)  Microcytic hyperchromic red cells 
B)  Normocytic hyperchromic red cells 
C)  Macrocytic hyperchromic red cells 
D)  No red cell morphology supports these indices. D

Question 2

What is the total volume of one side of a hemocytometer if the ruled area is 3 * 3 mm and the depth is 0.1 mm?

Accepted Answer

A)  0.3 mm3 
B)  0.9 mm3 
C)  1.0 mm3 
D)  9 mm3 
A)  0.3 mm3 
B)  0.9 mm3 
C)  1.0 mm3 
D)  9 mm3 B

Question 3

Which of the following red cell indices support macrocytic normochromic red cell morphology?

Accepted Answer

A

Question 4

On a standard reticulocyte preparation with new methylene blue, 100 cells are counted containing blue-stained granulofilamentous material. The red blood count is 3.22 * 1012/L, and the hematocrit is 30%. Calculate the absolute reticulocyte count.

Accepted Answer

A)  0.32* 1012/L 
B)  3.2 *1012/L 
C)  32.2*1012/L 
D)  0.99 * 1012/L 
A)  0.32* 1012/L 
B)  3.2 *1012/L 
C)  32.2*1012/L 
D)  0.99 * 1012/L

Question 5

What make RBCs settle faster through the plasma when inflammation or infection exist?

Accepted Answer

A)  Bacteria cause red cells to agglutinate and fall faster. 
B)  Increased number of neutrophils cause red cells to form rouleaux. 
C)  Inflammation makes the plasma less viscous. 
D)  Acute phase reactant proteins disrupt the electrical charge that normally separates cells. 
A)  Bacteria cause red cells to agglutinate and fall faster. 
B)  Increased number of neutrophils cause red cells to form rouleaux. 
C)  Inflammation makes the plasma less viscous. 
D)  Acute phase reactant proteins disrupt the electrical charge that normally separates cells.

Question 6

The MCV on a patient is calculated to be 115 fL and the MCHC is 35 g/dL. What, in general terms, must be true of the MCH if it is consistent with the previous two indices?

Accepted Answer

A)  It is impossible to know. 
B)  It should be in the reference range. 
C)  It should be below reference range. 
D)  It should be above reference range. 
A)  It is impossible to know. 
B)  It should be in the reference range. 
C)  It should be below reference range. 
D)  It should be above reference range.

Question 7

How do sickle cells or spherocytes interfere with the ESR?

Accepted Answer

A)  Prevent rouleaux formation, so falsely decrease 
B)  Encourage rouleaux formation, so falsely increase 
C)  Agglutinate and increase red cell mass, so falsely increase 
D)  Decrease plasma viscosity, so falsely decrease 
A)  Prevent rouleaux formation, so falsely decrease 
B)  Encourage rouleaux formation, so falsely increase 
C)  Agglutinate and increase red cell mass, so falsely increase 
D)  Decrease plasma viscosity, so falsely decrease

Question 8

A 1:20 dilution of blood is made for a white count, and the following number of cells counted in the four large corner mm2 of the hemocytometer: 12, 28, 18, and 15. What should be done next?

Accepted Answer

A)  Report the white count as 3.7 *109/L. 
B)  Clean the hemocytometer and recharge with a well-mixed dilution. 
C)  Increase the number of mm2 counted. 
D)  Compare this cell count to the one on the other side. 
A)  Report the white count as 3.7 *109/L. 
B)  Clean the hemocytometer and recharge with a well-mixed dilution. 
C)  Increase the number of mm2 counted. 
D)  Compare this cell count to the one on the other side.

Question 9

A manual WBC count is performed using a system that makes a 1:20 dilution of blood. When the cells in the four large corner mm2 of the hemocytometer are counted, the following numbers of cells are obtained: 8, 12, 9, and 11. What would be the next step?

Accepted Answer

A)  Count the cells in all 9 mm2 of the hemocytometer. 
B)  Redo the dilution of blood using a new 1:20 dilution. 
C)  Redo the dilution of blood using a 1:100 dilution. 
D)  Calculate the count using the values from the 4 mm2 and report the result. 
A)  Count the cells in all 9 mm2 of the hemocytometer. 
B)  Redo the dilution of blood using a new 1:20 dilution. 
C)  Redo the dilution of blood using a 1:100 dilution. 
D)  Calculate the count using the values from the 4 mm2 and report the result.

Question 10

The calculated indices on Mary Jones from Question 13 are:

Accepted Answer

The answer of The calculated indices on Mary Jones from...

Question 11

Which of the following could cause the microhematocrit to be falsely decreased?

Accepted Answer

A)  Specimen contaminated with intravenous fluid 
B)  Trapped plasma from abnormally shaped red cells 
C)  Dehydration 
D)  Insufficient centrifugation 
A)  Specimen contaminated with intravenous fluid 
B)  Trapped plasma from abnormally shaped red cells 
C)  Dehydration 
D)  Insufficient centrifugation

Question 12

In the four large corner squares of a hemocytometer, 95, 102, 105, and 98 white cells were counted from a 1:20 dilution. Select the correct interpretation for the white blood count calculated from these values.

Accepted Answer

A)  Normal for infant and elevated for an adult 
B)  Normal for any patient 
C)  Elevated for any patient 
D)  Normal for an infant and decreased for an adult 
A)  Normal for infant and elevated for an adult 
B)  Normal for any patient 
C)  Elevated for any patient 
D)  Normal for an infant and decreased for an adult

Question 13

Which of the following is a good use of the erythrocyte sedimentation rate (ESR)?

Accepted Answer

A)  Monitor patients with rheumatoid arthritis. 
B)  Diagnose acute appendicitis. 
C)  Diagnose multiple myeloma. 
D)  Monitor patients with osteoarthritis. 
A)  Monitor patients with rheumatoid arthritis. 
B)  Diagnose acute appendicitis. 
C)  Diagnose multiple myeloma. 
D)  Monitor patients with osteoarthritis.

Question 14

Which of the following could account for the red cell indices on Mary Jones reviewing Questions 13 through 15?

Accepted Answer

A)  Determining a likely cause is not possible. 
B)  An error in the hemoglobin probably has been made. 
C)  An error in the red cell count probably has been made. 
D)  An error in the microhematocrit probably has been made. 
A)  Determining a likely cause is not possible. 
B)  An error in the hemoglobin probably has been made. 
C)  An error in the red cell count probably has been made. 
D)  An error in the microhematocrit probably has been made.

Question 15

Twenty cells are counted in one of the nine large squares of a hemocytometer. The sample is diluted 1:10. How many cells are present per $\mu$L?

Accepted Answer

A)  200 
B)  1000 
C)  2000 
D)  20,000 
A)  200 
B)  1000 
C)  2000 
D)  20,000

Question 16

Which of the following red cell indices support normocytic normochromic red cell morphology?

Accepted Answer

The answer of Which of the following red cell indices...

Question 17

Which of the following would cause a manual cell count to be falsely high?

Accepted Answer

A)  Unopette not mixed before charging the hemocytometer 
B)  Not wiping excess blood from outside of pipette when filling 
C)  Using a smaller volume pipette than that specified by the Unopette system 
D)  Pulling blood out of the pipette when wiping away excess on the outside 
A)  Unopette not mixed before charging the hemocytometer 
B)  Not wiping excess blood from outside of pipette when filling 
C)  Using a smaller volume pipette than that specified by the Unopette system 
D)  Pulling blood out of the pipette when wiping away excess on the outside

Question 18

What error was probably made if red cells are seen when attempting to manually count white blood cell (WBCs) on a hemocytometer?

Accepted Answer

A)  The Unopette was not mixed before charging the hemocytometer. 
B)  Diluent was squeezed out and lost when filling the Unopette. 
C)  The wrong dilution was made. 
D)  The Unopette was not allowed to sit for 10 minutes for complete red blood cell (RB
C)  lysis. 
A)  The Unopette was not mixed before charging the hemocytometer. 
B)  Diluent was squeezed out and lost when filling the Unopette. 
C)  The wrong dilution was made. 
D)  The Unopette was not allowed to sit for 10 minutes for complete red blood cell (RB
C)  lysis.

Question 19

On a standard reticulocyte preparation with new methylene blue, 100 cells are counted that contain blue-stained granulofilamentous material. The red blood count is 3.22 *1012/L, and the hematocrit is 30%. Calculate the reticulocyte count as a percentage.

Accepted Answer

A)  0.1% 
B)  1.0% 
C)  10.0% 
D)  100.0% 
A)  0.1% 
B)  1.0% 
C)  10.0% 
D)  100.0%

Question 20

A manual hemoglobin is determined on a patient, and the dilution still appears turbid after letting it sit for 15 minutes. The stained blood film is examined, and sickle cells are observed on the blood film. Equal volumes of the diluted hemoglobin sample and water are mixed together, and the absorbance of this read on a spectrophotometer. How should the hemoglobin result be determined from the standard calibrated graph?

Accepted Answer

A)  Add the results from the turbid and second dilution together and divide by 2. 
B)  Multiply the result obtained from the graph on the diluted specimen by 2. 
C)  Divide the result obtained from the graph on the diluted specimen by 2. 
D)  There was no reason to perform this second dilution. 
A)  Add the results from the turbid and second dilution together and divide by 2. 
B)  Multiply the result obtained from the graph on the diluted specimen by 2. 
C)  Divide the result obtained from the graph on the diluted specimen by 2. 
D)  There was no reason to perform this second dilution.

Exam 14: Routine and Point-Of-Care Testing in Hematology: Manual and Semiautomated Methods

What red cell morphology would support the red cell indices calculated for Mary Jones in Question 14?

What is the total volume of one side of a hemocytometer if the ruled area is 3 * 3 mm and the depth is 0.1 mm?

Which of the following red cell indices support macrocytic normochromic red cell morphology?

On a standard reticulocyte preparation with new methylene blue, 100 cells are counted containing blue-stained granulofilamentous material. The red blood count is 3.22 * 1012/L, and the hematocrit is 30%. Calculate the absolute reticulocyte count.

What make RBCs settle faster through the plasma when inflammation or infection exist?

The MCV on a patient is calculated to be 115 fL and the MCHC is 35 g/dL. What, in general terms, must be true of the MCH if it is consistent with the previous two indices?

How do sickle cells or spherocytes interfere with the ESR?

A 1:20 dilution of blood is made for a white count, and the following number of cells counted in the four large corner mm2 of the hemocytometer: 12, 28, 18, and 15. What should be done next?

A manual WBC count is performed using a system that makes a 1:20 dilution of blood. When the cells in the four large corner mm2 of the hemocytometer are counted, the following numbers of cells are obtained: 8, 12, 9, and 11. What would be the next step?

The calculated indices on Mary Jones from Question 13 are:

Which of the following could cause the microhematocrit to be falsely decreased?

In the four large corner squares of a hemocytometer, 95, 102, 105, and 98 white cells were counted from a 1:20 dilution. Select the correct interpretation for the white blood count calculated from these values.

Which of the following is a good use of the erythrocyte sedimentation rate (ESR)?

Which of the following could account for the red cell indices on Mary Jones reviewing Questions 13 through 15?

Twenty cells are counted in one of the nine large squares of a hemocytometer. The sample is diluted 1:10. How many cells are present per μ\muμ L?

Which of the following red cell indices support normocytic normochromic red cell morphology?

Which of the following would cause a manual cell count to be falsely high?

What error was probably made if red cells are seen when attempting to manually count white blood cell (WBCs) on a hemocytometer?

On a standard reticulocyte preparation with new methylene blue, 100 cells are counted that contain blue-stained granulofilamentous material. The red blood count is 3.22 *1012/L, and the hematocrit is 30%. Calculate the reticulocyte count as a percentage.

An Overview of Clinical Laboratory Hematology

Safety in the Hematology Laboratory

Specimen Collection

Care and Use of the Microscope

Quality Assurance in Hematology and Hemostasis Testing

Cellular Structure and Function

Hematopoiesis

Erythrocyte Production and Destruction

Energy Metabolism and Membrane Physiology of the Erythrocyte

Hemoglobin Metabolism

Iron Metabolism

Leukocyte Development, Kinetics, and Functions

Platelet Production, Structure, and Function

Examination of the Peripheral Blood Film and Correlation With the Complete Blood Count

Bone Marrow Examination

Body Fluids in the Hematology Laboratory

Anemias: Red Blood Cell Morphology and Approach to Diagnosis

Disorders of Iron and Heme Metabolism

Anemias Caused by Defects of Dna Metabolism

Bone Marrow Failure

Introduction to Increased Destruction of Erythrocytes

Intrinsic Defects Leading to Increased Erythrocyte Destruction

Extrinsic Defects Leading to Increased Erythrocytedestructionnonimmune Causes

Extrinsic Defects Leading to Increased Erythrocytedestructionimmune Causes

Hemoglobinopathies Structural Defects in Hemoglobin

Thalassemias

Nonmalignant Leukocyte Disorders

Introduction to Leukocyte Neoplasms

Cytochemistry

Cytogenetics

Molecular Diagnostics in the Clinical Laboratory

Flow Cytometric Analysis in Hematologic Disorders

Myeloproliferative Neoplasms

Myelodysplastic Syndromes

Acute Leukemias

Mature Lymphoid Neoplasms

Pediatric and Geriatric Hematology

Automated Cell-Counting Instrumentation

Normal Hemostasis and Coagulation

Hemorrhagic Coagulation Disorders

Thrombosis Risk Testing

Thrombocytopenia and Thrombocytosis

Qualitative Disorders of Platelets and Vasculature

Laboratory Evaluation of Hemostasis

Monitoring Antithrombotic Therapies

Coagulation Instrumentation

Filters

On a standard reticulocyte preparation with new methylene blue, 100 cells are counted containing blue-stained granulofilamentous material. The red blood count is 3.22 * 10^12/L, and the hematocrit is 30%. Calculate the absolute reticulocyte count.

A 1:20 dilution of blood is made for a white count, and the following number of cells counted in the four large corner mm² of the hemocytometer: 12, 28, 18, and 15. What should be done next?

A manual WBC count is performed using a system that makes a 1:20 dilution of blood. When the cells in the four large corner mm² of the hemocytometer are counted, the following numbers of cells are obtained: 8, 12, 9, and 11. What would be the next step?

Twenty cells are counted in one of the nine large squares of a hemocytometer. The sample is diluted 1:10. How many cells are present per $\mu$ L?

On a standard reticulocyte preparation with new methylene blue, 100 cells are counted that contain blue-stained granulofilamentous material. The red blood count is 3.22 *10¹²/L, and the hematocrit is 30%. Calculate the reticulocyte count as a percentage.