Question 1

What immersion oil is used most commonly in hematology?

Accepted Answer

A)  Type A 
B)  Type B 
C)  Type C 
D)  Type D 
A)  Type A 
B)  Type B 
C)  Type C 
D)  Type D B

Question 2

An object is brought into focus using the 10*objective lens. When the 40* objective lens is moved into position, only a minor adjustment using the fine focus adjustment is required to bring it clearly into focus. What is the term used to describe lenses with this feature?

Accepted Answer

A)  Parcentric 
B)  Achromatic 
C)  Plan-achromatic 
D)  Parfocal 
A)  Parcentric 
B)  Achromatic 
C)  Plan-achromatic 
D)  Parfocal D

Question 3

What converts for curvature in a field so that a flat field with uniform focus is seen?

Accepted Answer

A)  Achromatic objective lens 
B)  Plan-achromatic objective lens 
C)  Spherical correction ocular lens 
D)  Blue filter when placed above the condenser 
A)  Achromatic objective lens 
B)  Plan-achromatic objective lens 
C)  Spherical correction ocular lens 
D)  Blue filter when placed above the condenser B

Question 4

MATCHING
Match each lettered type of microscope to its clinical use

-_____ Routine differentials

Accepted Answer

A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield 
A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield

Question 5

MATCHING
Match each lettered type of microscope to its clinical use.

-_____ Used with fluorochrome dyes to identify lymphocyte subsets

Accepted Answer

A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield 
A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield

Question 6

What should be adjusted if two overlapping images, instead of only one, are seen when looking through a microscope?

Accepted Answer

A)  Field diaphragm 
B)  Fine adjustment knob 
C)  Condenser knob 
D)  Interpupillary control 
A)  Field diaphragm 
B)  Fine adjustment knob 
C)  Condenser knob 
D)  Interpupillary control

Question 7

Where should the condenser be located when using the *100 oil objective?

Accepted Answer

A)  Lowest position 
B)  Highest position 
C)  Middle position 
D)  Variable depending on amount of light desired 
A)  Lowest position 
B)  Highest position 
C)  Middle position 
D)  Variable depending on amount of light desired

Question 8

Which of the following would be least likely to cause a "fuzzy" image on oil power?

Accepted Answer

A)  Using a plan-achromatic objective lens 
B)  Objective not screwed in tightly 
C)  Not enough oil on the slide when using oil immersion lens 
D)  Focus controls not adjusted 
A)  Using a plan-achromatic objective lens 
B)  Objective not screwed in tightly 
C)  Not enough oil on the slide when using oil immersion lens 
D)  Focus controls not adjusted

Question 9

Which of the following microscopic procedures requires high contrast and lower light?

Accepted Answer

A)  Examining unstained objects (e.g., urine sediments) 
B)  Evaluating red cell morphology 
C)  Manual differentials 
D)  Platelet estimates 
A)  Examining unstained objects (e.g., urine sediments) 
B)  Evaluating red cell morphology 
C)  Manual differentials 
D)  Platelet estimates

Question 10

What is the most likely reason for cells on a hemocytometer appearing "fuzzy" when switching from low power to the *40 objective?

Accepted Answer

A)  Condenser not lowered enough 
B)  Hemocytometer not cleaned properly 
C)  Oil on the 40* lens 
D)  Cells not allowed to settle 
A)  Condenser not lowered enough 
B)  Hemocytometer not cleaned properly 
C)  Oil on the 40* lens 
D)  Cells not allowed to settle

Question 11

MATCHING
Match each lettered type of microscope to its clinical use

-_____ Viewing spirochetes in microbiology

Accepted Answer

A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield 
A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield

Question 12

What can increase the resolution of an object under the microscope?

Accepted Answer

A)  Lowering the condenser 
B)  Increasing the combined magnification provided by the ocular and objective lenses 
C)  Enlarging the numeric aperture of the objective lens 
D)  Adjusting the focus controls 
A)  Lowering the condenser 
B)  Increasing the combined magnification provided by the ocular and objective lenses 
C)  Enlarging the numeric aperture of the objective lens 
D)  Adjusting the focus controls

Question 13

An image viewed under the microscope is clearly seen under both the 10*and 40*objectives, but it is not visible under *100. What is the most likely reason for this?

Accepted Answer

A)  Oil is too thick on the slide. 
B)  The slide is placed upside down on the stage. 
C)  The slide has a coverslip on it. 
D)  No oil is on the slide. 
A)  Oil is too thick on the slide. 
B)  The slide is placed upside down on the stage. 
C)  The slide has a coverslip on it. 
D)  No oil is on the slide.

Question 14

Koehler illumination:

Accepted Answer

A)  Is best achieved using a tungsten-halogen lightbulb 
B)  Allows the brightest amount of light to be in the center of a field 
C)  Adjusts for even light across a field 
D)  Changes the intensity of the light across a field 
A)  Is best achieved using a tungsten-halogen lightbulb 
B)  Allows the brightest amount of light to be in the center of a field 
C)  Adjusts for even light across a field 
D)  Changes the intensity of the light across a field

Question 15

The device used to assist in finding a cell of interest at a later time is called a:

Accepted Answer

A)  Microlocator 
B)  Micrometer 
C)  Reticle 
D)  Hemocytometer 
A)  Microlocator 
B)  Micrometer 
C)  Reticle 
D)  Hemocytometer

Question 16

How should a microscope be carried?

Accepted Answer

A)  Both hands supporting the base 
B)  Both hands around the arm 
C)  One hand around the arm and the other holding the oculars in place 
D)  One hand under the base and the other around the arm 
A)  Both hands supporting the base 
B)  Both hands around the arm 
C)  One hand around the arm and the other holding the oculars in place 
D)  One hand under the base and the other around the arm

Question 17

Why is it not advisable to interchange one ocular lens with another from a different brand microscope?

Accepted Answer

A)  The two ocular lenses in a microscope are optically matched with one another. 
B)  The fitting for the ocular lenses varies, so they will not fit in another microscope. 
C)  They cannot be removed from the microscope. 
D)  They are fragile and are likely to break when removed from the microscope. 
A)  The two ocular lenses in a microscope are optically matched with one another. 
B)  The fitting for the ocular lenses varies, so they will not fit in another microscope. 
C)  They cannot be removed from the microscope. 
D)  They are fragile and are likely to break when removed from the microscope.

Question 18

What solution is best for cleaning lenses?

Accepted Answer

A)  Commercial glass cleaning solution (e.g., Windex) 
B)  Water 
C)  Benzene 
D)  Xylol 
A)  Commercial glass cleaning solution (e.g., Windex) 
B)  Water 
C)  Benzene 
D)  Xylol

Question 19

MATCHING
Match each lettered type of microscope to its clinical use

-_____ Identifying crystals in synovial fluid

Accepted Answer

A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield 
A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield

Question 20

MATCHING
Match each lettered type of microscope to its clinical use

-_____ Counting platelets manually

Accepted Answer

A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield 
A) Phase contrast 
B) Darkfield 
C) Polarized light 
D) Fluorescent 
E) Brightfield

Exam 4: Care and Use of the Microscope

What immersion oil is used most commonly in hematology?

An object is brought into focus using the 10*objective lens. When the 40* objective lens is moved into position, only a minor adjustment using the fine focus adjustment is required to bring it clearly into focus. What is the term used to describe lenses with this feature?

What converts for curvature in a field so that a flat field with uniform focus is seen?

MATCHING Match each lettered type of microscope to its clinical use -_____ Routine differentials

MATCHING Match each lettered type of microscope to its clinical use. -_____ Used with fluorochrome dyes to identify lymphocyte subsets

What should be adjusted if two overlapping images, instead of only one, are seen when looking through a microscope?

Where should the condenser be located when using the *100 oil objective?

Which of the following would be least likely to cause a "fuzzy" image on oil power?

Which of the following microscopic procedures requires high contrast and lower light?

What is the most likely reason for cells on a hemocytometer appearing "fuzzy" when switching from low power to the *40 objective?

MATCHING Match each lettered type of microscope to its clinical use -_____ Viewing spirochetes in microbiology

What can increase the resolution of an object under the microscope?

An image viewed under the microscope is clearly seen under both the 10*and 40*objectives, but it is not visible under *100. What is the most likely reason for this?

Koehler illumination:

The device used to assist in finding a cell of interest at a later time is called a:

How should a microscope be carried?

Why is it not advisable to interchange one ocular lens with another from a different brand microscope?

What solution is best for cleaning lenses?

MATCHING Match each lettered type of microscope to its clinical use -_____ Identifying crystals in synovial fluid

MATCHING Match each lettered type of microscope to its clinical use -_____ Counting platelets manually

An Overview of Clinical Laboratory Hematology

Safety in the Hematology Laboratory

Specimen Collection

Quality Assurance in Hematology and Hemostasis Testing

Cellular Structure and Function

Hematopoiesis

Erythrocyte Production and Destruction

Energy Metabolism and Membrane Physiology of the Erythrocyte

Hemoglobin Metabolism

Iron Metabolism

Leukocyte Development, Kinetics, and Functions

Platelet Production, Structure, and Function

Routine and Point-Of-Care Testing in Hematology: Manual and Semiautomated Methods

Examination of the Peripheral Blood Film and Correlation With the Complete Blood Count

Bone Marrow Examination

Body Fluids in the Hematology Laboratory

Anemias: Red Blood Cell Morphology and Approach to Diagnosis

Disorders of Iron and Heme Metabolism

Anemias Caused by Defects of Dna Metabolism

Bone Marrow Failure

Introduction to Increased Destruction of Erythrocytes

Intrinsic Defects Leading to Increased Erythrocyte Destruction

Extrinsic Defects Leading to Increased Erythrocytedestructionnonimmune Causes

Extrinsic Defects Leading to Increased Erythrocytedestructionimmune Causes

Hemoglobinopathies Structural Defects in Hemoglobin

Thalassemias

Nonmalignant Leukocyte Disorders

Introduction to Leukocyte Neoplasms

Cytochemistry

Cytogenetics

Molecular Diagnostics in the Clinical Laboratory

Flow Cytometric Analysis in Hematologic Disorders

Myeloproliferative Neoplasms

Myelodysplastic Syndromes

Acute Leukemias

Mature Lymphoid Neoplasms

Pediatric and Geriatric Hematology

Automated Cell-Counting Instrumentation

Normal Hemostasis and Coagulation

Hemorrhagic Coagulation Disorders

Thrombosis Risk Testing

Thrombocytopenia and Thrombocytosis

Qualitative Disorders of Platelets and Vasculature

Laboratory Evaluation of Hemostasis

Monitoring Antithrombotic Therapies

Coagulation Instrumentation

Filters

An object is brought into focus using the 10objective lens. When the 40 objective lens is moved into position, only a minor adjustment using the fine focus adjustment is required to bring it clearly into focus. What is the term used to describe lenses with this feature?

An image viewed under the microscope is clearly seen under both the 10and 40objectives, but it is not visible under *100. What is the most likely reason for this?