Question 1

All of the following are true concerning hematopoietic stem cells except:

Accepted Answer

A)  They express both CD34 and CD117 (c-kit). 
B)  They lose some antigens as they mature. 
C)  They express both CD4 and CD34. 
D)  They gain lineage-specific antigens as they mature. 
A)  They express both CD34 and CD117 (c-kit). 
B)  They lose some antigens as they mature. 
C)  They express both CD4 and CD34. 
D)  They gain lineage-specific antigens as they mature. C

Question 2

Myeloid stem cells and blasts express which of the following on their surface?

Accepted Answer

A)  CD4 
B)  CD8 
C)  CD34 
D)  Terminal deoxynucleotidyl transferase (TdT) 
A)  CD4 
B)  CD8 
C)  CD34 
D)  Terminal deoxynucleotidyl transferase (TdT) C

Question 3

What cell type expresses CD20 and contains immunoglobulin light chains on the surface but not in the cytoplasm?

Accepted Answer

A)  Helper T cells (TH) 
B)  T suppressor cells 
C)  Immature B cells 
D)  Mature B cells 
A)  Helper T cells (TH) 
B)  T suppressor cells 
C)  Immature B cells 
D)  Mature B cells D

Question 4

The presence of TdT is characteristic of progenitors of which lineage?

Accepted Answer

A)  Megakaryocytes and platelets 
B)  Monocytes 
C)  Erythrocytes 
D)  Lymphocytes 
A)  Megakaryocytes and platelets 
B)  Monocytes 
C)  Erythrocytes 
D)  Lymphocytes

Question 5

Which of the following is true regarding the relationship between flow cytometry and the microscopic evaluation of leukemic cells?

Accepted Answer

A)  Flow cytometry has made microscopic analysis outmoded. 
B)  Methods are best used together to establish a diagnosis. 
C)  More cells can be analyzed by looking under the microscope. 
D)  The human eye is more sensitive than flow cytometry and better at finding weak antigenic expression. 
A)  Flow cytometry has made microscopic analysis outmoded. 
B)  Methods are best used together to establish a diagnosis. 
C)  More cells can be analyzed by looking under the microscope. 
D)  The human eye is more sensitive than flow cytometry and better at finding weak antigenic expression.

Question 6

Forward scatter is related to what cellular feature?

Accepted Answer

A)  Density 
B)  Size 
C)  Granularity 
D)  Complexity 
A)  Density 
B)  Size 
C)  Granularity 
D)  Complexity

Question 7

Which of the following is evaluated using fluorochrome dyes that are bound to monoclonal antibodies?

Accepted Answer

A)  Membrane antigens 
B)  Nuclear complexity 
C)  Number of lymphocytes 
D)  Cell size 
A)  Membrane antigens 
B)  Nuclear complexity 
C)  Number of lymphocytes 
D)  Cell size

Question 8

What marker is most closely related to disease progression in acquired immune deficiency syndrome (AIDS) patients?

Accepted Answer

A)  CD3 
B)  CD4 
C)  CD8 
D)  CD10 
A)  CD3 
B)  CD4 
C)  CD8 
D)  CD10

Question 9

What is an early marker of erythroid precursors?

Accepted Answer

A)  CD45 
B)  CD71 
C)  CD34 
D)  CD8 
A)  CD45 
B)  CD71 
C)  CD34 
D)  CD8

Question 10

A patient has a white cell count of 12.6 *109/L, hematocrit of 28%, and platelet count of 27*109/L. Examination of a Wright-stained blood film reveals that almost all of the white cells in peripheral blood are blasts; the bone marrow is hypercellular with very little fat and is packed with blasts. Flow cytometry is performed on the peripheral blood cells, and a large cluster of cells is present that mark with CD20 and have monoclonal$\kappa$ light chains present on their surface. This patient has a:

Accepted Answer

A)  Myeloid leukemia 
B)  Mature B-cell malignancy 
C)  Reactive B-cell hyperplasia 
D)  Mature T-cell malignancy 
A)  Myeloid leukemia 
B)  Mature B-cell malignancy 
C)  Reactive B-cell hyperplasia 
D)  Mature T-cell malignancy

Question 11

Staining a sample with deoxyribonucleic acid (DNA) dyes such as propidium iodide is done to determine cell:

Accepted Answer

A)  Viability 
B)  Ploidy 
C)  Lineage 
D)  Chromosome number 
A)  Viability 
B)  Ploidy 
C)  Lineage 
D)  Chromosome number

Question 12

Hydrodynamic focusing in flow cytometry is important because it:

Accepted Answer

A)  Directs the laser light 
B)  Intensifies the laser light with mirrors 
C)  Collects the scattered light 
D)  Creates a core of individually aligned cells 
A)  Directs the laser light 
B)  Intensifies the laser light with mirrors 
C)  Collects the scattered light 
D)  Creates a core of individually aligned cells

Exam 33: Flow Cytometric Analysis in Hematologic Disorders

All of the following are true concerning hematopoietic stem cells except:

Myeloid stem cells and blasts express which of the following on their surface?

What cell type expresses CD20 and contains immunoglobulin light chains on the surface but not in the cytoplasm?

The presence of TdT is characteristic of progenitors of which lineage?

Which of the following is true regarding the relationship between flow cytometry and the microscopic evaluation of leukemic cells?

Forward scatter is related to what cellular feature?

Which of the following is evaluated using fluorochrome dyes that are bound to monoclonal antibodies?

What marker is most closely related to disease progression in acquired immune deficiency syndrome (AIDS) patients?

What is an early marker of erythroid precursors?

Staining a sample with deoxyribonucleic acid (DNA) dyes such as propidium iodide is done to determine cell:

Hydrodynamic focusing in flow cytometry is important because it:

An Overview of Clinical Laboratory Hematology

Safety in the Hematology Laboratory

Specimen Collection

Care and Use of the Microscope

Quality Assurance in Hematology and Hemostasis Testing

Cellular Structure and Function

Hematopoiesis

Erythrocyte Production and Destruction

Energy Metabolism and Membrane Physiology of the Erythrocyte

Hemoglobin Metabolism

Iron Metabolism

Leukocyte Development, Kinetics, and Functions

Platelet Production, Structure, and Function

Routine and Point-Of-Care Testing in Hematology: Manual and Semiautomated Methods

Examination of the Peripheral Blood Film and Correlation With the Complete Blood Count

Bone Marrow Examination

Body Fluids in the Hematology Laboratory

Anemias: Red Blood Cell Morphology and Approach to Diagnosis

Disorders of Iron and Heme Metabolism

Anemias Caused by Defects of Dna Metabolism

Bone Marrow Failure

Introduction to Increased Destruction of Erythrocytes

Intrinsic Defects Leading to Increased Erythrocyte Destruction

Extrinsic Defects Leading to Increased Erythrocytedestructionnonimmune Causes

Extrinsic Defects Leading to Increased Erythrocytedestructionimmune Causes

Hemoglobinopathies Structural Defects in Hemoglobin

Thalassemias

Nonmalignant Leukocyte Disorders

Introduction to Leukocyte Neoplasms

Cytochemistry

Cytogenetics

Molecular Diagnostics in the Clinical Laboratory

Myeloproliferative Neoplasms

Myelodysplastic Syndromes

Acute Leukemias

Mature Lymphoid Neoplasms

Pediatric and Geriatric Hematology

Automated Cell-Counting Instrumentation

Normal Hemostasis and Coagulation

Hemorrhagic Coagulation Disorders

Thrombosis Risk Testing

Thrombocytopenia and Thrombocytosis

Qualitative Disorders of Platelets and Vasculature

Laboratory Evaluation of Hemostasis

Monitoring Antithrombotic Therapies

Coagulation Instrumentation

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