Exam 8: Nucleotides and Nucleic Acids

According to the sequence below (note that the DNA is written in the 5' $\rightarrow$ 3' direction, and the gene of interest is underlined), the BEST reverse primer to use for this gene is:

When performing PCR, what is the advantage to using a DNA polymerase isolated from a thermophilic organism?

Which DNA primer would have the HIGHEST melting temperature?

Which statement is NOT true of all naturally occurring DNA?

The difference between a ribonucleotide and a deoxyribonucleotide is:

The image below shows a DNA sequencing gel using the Sanger method. The top of the gel is the anode and the bottom is the cathode. Which band represents the shortest and longest fragments of DNA?

The phosphodiester bond that joins adjacent nucleotides in DNA:

For the oligoribonucleotide pACGUAC, the nucleotide at the:

Which conformation is NOT permitted due to steric interference?

In the Watson-Crick structure of DNA, the:

Speculate why adenosine, rather than another large molecule, is used often as a cofactor in a nucleotide-binding fold of different enzymes.

According to the sequence below (note that the DNA is written in the 5' $\rightarrow$ 3' direction, and the gene of interest is underlined), the BEST forward primer to use for this gene is:

Compounds that generate nitrous acid (such as nitrites, nitrates, and nitrosamines) change DNA molecules by:

Purines have ____ ring(s), (each) containing _____ nitrogen(s), whereas pyrimidines have _____ ring(s), (each) containing _____ nitrogens.

The phosphodiester bonds that link adjacent nucleotides in both RNA and DNA:

Based on the spontaneous deamination of cytosine, explain why it is advantageous for DNA to contain thymine, not uracil.

Based on Chargaff's rules, which base compositions for double-stranded DNA are possible? %A %G %C %T %U

What happens in automated Sanger DNA sequencing reaction if you forget to add a) all fluorescent ddNTPs? b) fluorescent ddGTP? c) all dNTPs? d) dGTP?